Summary
Electron microprobe analysis was used to examine relative amounts of selected accessory elements (Ca, Mg, K, Na, P, Mn, and Fe) associated with silicon in silica cells in leaf epidermal cells ofCyperus alternifolius. In the leaf epidermal cells, silicon is localised in significant quantities in silica cells and in a few long epidermal cells. Silicon could not be detected in the stomatal apparatuses. The accumulation of significant amounts of silicon in the silica cell appears to retard the accumulation of K and to enhance the accumulation of Ca, Mg, Mn, Fe, and P. Sodium was detected in very small traces in the silica cells. The possible functional significance of the altered deposition patterns for these elements inCyperus is discussed.
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Anderson, C. A., An introduction to the electron probe microanalyzer and its application to biochemistry, pp, 147–270.In Methods of Biochemical Analysis, vol.15. Interscience, New York (1967).
Brenchley, W. E., Maskell, E. J., and Warrington, K., The interrelations between silicon and other elements in plant nutrition. Applied Biol.14, 45–82 (1927).
Butkwitsch, W. S., and Butkwitsch, W. W., Zur Frage nach der Rolle des ‘Donnanscchen Membrangleichgewichts’ bei osmotischen Vorgangen in lebenden Zellen. Biochem. Z.161, 468–487 (1925).
Carlisle, E. M., Silicon: a possible factor in bone calcification. Science167, 279–280 (1970).
Esau, K., Plant Anatomy, 2nd Ed. p. 767. John Wiley and Sons, Inc., New York (1965).
Islam, A. and Saha, R. C., Effects of silicon on the chemical composition of rice plants. Plant and Soil30, 446–458 (1969).
Jones, L. P. H., Milne, A. A., and Wadham, S. M., Studies of silica in the oat plant. II. Distribution of the silica in the plant. Plant and Soil18, 358–371 (1963).
Kaufman, P. B., Bigelow, W. C., Petering, L. B., and Drogosz, F. B., Silica in developing epidermal cells ofAvena internodes: electron microprobe analysis. Science166, 1015–1017 (1969).
Kaufman, P. B., Bigelow, W. C., Schmid, R., and Ghosheh, N. S., Electron microprobe analysis of silica in epidermal cells of Equisetum. Amer. J. Bot.58, 309–316 (1971).
Laroche, J., Localisation de la silice per le microanalyseur à sonde électronique. Compt. Rend. Acad. Sci. (Paris)265, 1695–1697 (1967).
Laroche, J., Contribution à l'étude de l'Equisetum arvense L. III. Recherches sur la nature et la localisation de la silice chez le sporophyte. Rev. Gén. Botan.75, 65–116 (1968).
Laroche, J., État de la silice sur et dans la membrane épidermique des organes aériens stériles d'Equisetum arvense L. Rev. Gén. Botan76, 483–489 (1969).
Lewin, J. C., and Reimann, B. E. F., Silicon and plant growth. Ann. Rev. Plant Physiol.20, 289–304 (1969).
Mehra, P. N. and Sharma, O. P., Epidermal silica cells in the Cyperaceae. Botan. Gaz.126, 53–58 (1965).
Metcalfe, C. R., The anatomical approach to systematics: General introduction with special reference to recent work on monocotyledons, pp. 146–150.In Recent Advances in Botany. Univ. of Toronto Press, Toronto (1961).
Netolitzky, F.,In Handbuch der Pflanzenanatomie, K. Linsbauer ed. Band3/Ia (Lief 25), 1–80. Borntrager, Berlin (1929).
Okuda, A. and Takahashi, E.,In The Mineral Nutrition of the Rice Plant, 123–146 (Proc. Symp. Intern. Rice Research Inst., Feb. 1964). John Hopkins Press, Baltimore (1965).
Parry, D. W. and Smithson, F., Techniques for studying opaline silica in grass leaves. Ann. Botany22, 543–549 (1958).
Rothbuhr, L. and Scott, F., A study of the uptake of silicon and phosphorus by wheat plants, with radiochemical methods. Biochem. J.65, 241–245 (1957).
Sangster, A. G., Studies of opaline silica deposits in the leaf ofSieglingia decumbens L. ‘Bernh.’ using the scanning electron microscope. Ann. Botany32 (126), 237–240 (1968).
Schafer, P. W. and Chandler, J. A., Electron probe x-ray microanalysis of a normal centriole. Science170, 1204–1205 (1970).
Soni, S. L., Kaufman, P. B., and Bigelow, W. C., Electron microprobe analysis of the distribution of silicon in leaf epidermal cells of the oat plant (Avena sativa L.). Phytomorphology21, 348–361 (1971).
Zelitch, I., Stomatal control. Ann. Rev. Plant Physiol. Vol.20, 329–350 (1969)
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Soni, S.L., Kaufman, P.B. & Bigelow, W.C. Electron microprobe analysis of silica cells in leaf epidermal cells ofCyperus alternifolius . Plant Soil 36, 121–128 (1972). https://doi.org/10.1007/BF01373462
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DOI: https://doi.org/10.1007/BF01373462