Summary
Excision repair was studied in normal human and ataxia telangiectasia (AT) cells proficient in repair of UV and its mimetic chemicals, and in xeroderma pigmentosum group C (XP C) cells (deficient in repair of UV and its mimetics), after treatment with several combinations of chemical carcinogens, by the photolysis of bromodeoxyuridine incorporated into parental DNA during repair. Results indicate that repair was additive in AT, and XP C cells treated with N-acetoxy-2-acetylaminofluorene (AAAF) plus ethyl methanesulfonate (EMS) or methyl methanesulfonate (MMS) indicating that there are different rate limiting steps for removal of both types of damage. Data on the combinations of 4-nitroquinoline 1-oxide (4NQO) plus MMS or EMS are difficult to interpret, but they do not indicate inhibition of DNA repair.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Ahmed, F. E., Setlow, R. B.: Excision repair in ataxia telangiectasia, Fanconi's anemia, Cockayne syndrome and Bloom's syndrome after treatment with ultraviolet radiation and N-acetoxy-2-acetylaminofluorene. Biochim. Biophys. Acta521, 805–817 (1978)
Ahmed, F. E., Setlow, R. B.: DNA repair in xeroderma pigmentosum cells treated with combinations of ultraviolet radiation and N-acetoxy-2-acetylaminofluorene. Cancer Res.39, 471–479 (1979)
Ahmed, F. E., Setlow, R. B.: Saturation of DNA repair in mammalian cells. Photochem. Photobiol.29, 983–989 (1979)
Ahmed, F. E., Setlow, R. B.: DNA excision in repair proficient and deficient human cells treated with combinations of ultraviolet radiation and acridine mustard (ICR-170) or 4-nitroquinoline 1-oxide. Chem. Biol. Interact.29, 31–42 (1980)
Brown, A. J., Fickel, T. H., Cleaver, J. E., Lohman, P. H. M., Wade, M. H., Waters, R.: Overlapping pathways for repair of damage from ultraviolet light and chemical carcinogens in human fibroblasts. Cancer Res.39, 2522–2527 (1979)
Chen, T. R.: Microscopic demonstration ofMycoplasma contaminants in cell cultures and cell culture media. Tiss. Cult. Assoc. Man.1, 229–232 (1975)
Clarkson, J. M., Evans, H. J.: Unscheduled DNA synthesis in human leucocytes after exposure to UV light,γ-rays and chemical mutagens. Mutat. Res.14, 413–430 (1971)
Cleaver, J. E.: Repair of alkylation damage in ultraviolet-sensitive (xeroderma pigmentosum) human cells. Mutat. Res.12, 453–462 (1971)
Elkind, M. M., Kamper, C.: Two forms of repair of DNA in mammalian cells following irradiation. Biophys. J.10, 237–245 (1970)
Jagger, J.: Introduction to Research in Ultraviolet Photobiology. p. 131. Engelwood Cliffs, N.J.: Prentice-Hall, Inc. 1967
Johns, H. E., Rauth, A. M.: Theory and design of high intensity, U.V. monochromators for photobiology and photochemistry. Photochem. Photobiol.4, 673–692 (1965)
Paterson, M. C., Smith, B. P., Lohman, P. H. M., Anderson, A. K., Fishman, L.: Defective excision repair of gamma ray-damaged DNA in human (Ataxia telangiectasia) fibroblasts. Nature260, 444–447 (1976)
Regan, J. D., Setlow, R. B.: Two forms of repair in DNA of human cells damaged by chemical carcinogens and mutagens. Cancer Res.34, 3318–3325 (1974)
Regan, J. D., Setlow, R. B., Ley, R. D.: Normal and defective repair of damaged DNA in human cells: a sensitive assay utilizing the photolysis of bromodeoxyuridine. Proc. Natl. Acad. Sci. U.S.A.68, 708–712 (1971)
Setlow, R. B., Faulcon, F. M., Regan, J. D.: Defective repair of gamma-ray-induced DNA damage in xeroderma pigmentosum cells. Int. J. Radiat. Biol.29, 125–136 (1976)
Setlow, R. B., Regan, J. D., German, J., Carrier, W. L.: Evidence that xeroderma pigmentosum cells do not perform the first step in the repair of ultraviolet damage to their DNA. Proc. Natl. Acad. Sci. U.S.A.64, 1035–1041 (1969)
Stich, H. F., San, R. H. C.: Reduced DNA repair synthesis in xeroderma pigmentosum cells exposed to the oncogenic 4-nitroquinoline 1-oxide and hydroxyaminoquinoline 1-oxide. Mutat. Res.13, 279–282 (1971)
Stick, H. F., San, R. H. C., Kawazoe, Y.: DNA repair synthesis in mammalian cells exposed to a series of oncogenic and non-oncogenic derivatives of 4-nitroquinoline 1-oxide. Nature229, 416–419 (1971)
Wheeler, K. T., Levine, V. A., Deen, D. F.: The concept of drug dose for in vitro studies with chemotherapeutic agents. Radiat. Res.76, 441–458 (1978)
Author information
Authors and Affiliations
Additional information
Research carried out under the auspices of the U.S. Dept. of Energy
Rights and permissions
About this article
Cite this article
Ahmed, F.E. The combined action of chemical carcinogens on DNA repair in human cells. Radiat Environ Biophys 18, 57–64 (1980). https://doi.org/10.1007/BF01324374
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01324374