Summary
Denatured calf-thymus DNA was X-irradiated, coupled to methylated bovine serum albumin and injected into rabbits to study the possible formation of specific antibodies. The serological activity was tested by a modified Farr-test, a micro complement binding reaction and by caesium chloride isopycnic ultracentrifugation. It was found with all assays that the immunological reaction was mainly due to unspecific DNA-binding and only a very small amount to radiation products. It seems, therefore, that this approach is not suitable for the analytical investigation of DNA damage produced by ionizing radiation.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Cornelis, J. J., Rommelaere, J., Urbain, J., Errera, M.: A sensitive method for measuring pyrimidine dimers in situ. Photochem. Photobiol.26, 241–246 (1977)
Farr, R. S.: A quantitative immunochemical measure of the primary interaction between J*BSA and antibody. J. Infect. Dis. 103–262 (1958)
Fink, A., Hotz, G.: Immunological reaction of UV-induced radiation damage in coliphage DNA. Z. Naturforsch.32c, 544–549 (1977)
Hotz, G.: Immunological recognition of specific antigenic determinants in UV- and Gamma-irradiated phage-DNA. Radiat. Environ. Biophys.12, 41–50 (1975)
Levine, L., Seaman, E., Hammerschlag, E., van Vunakis, H.: Antibodies to photoproducts of deoxyribonucleic acids irradiated with ultraviolet light. Science153, 1666–1667 (1966)
Lewis, H. L., Muhleman, D. R., Ward, J. F.: Serologic assays forγ-irradiated DNA base damage. Radiat. Res.67, 635 (1976)
Lucas, C. J.: Immunological demonstration of the disappearance of pyrimidine dimers from nuclei of cultured human cells. Exp. Cell Res.74, 480–486 (1972)
Marmur, J.: A procedure for the isolation of DNA from microorganisms. J. Mol. Biol.3, 208–219 (1961)
Natali, P. G., Tan, E. M.: Immunological detection of thymidine photoproduct formation in vivo. Radiat. Res.46, 506–518 (1971)
Plescia, O. J., Braun, W. Palczuk, N. C.: Production of antibodies to denatured deoxyribonucleic acid (DNA). Biochemistry52, 279–285 (1964)
Saenko A. S., Ilyncia, T. P., Podgorodnichenko, V. K., Porerenny, A. M.: Simple immunochemical methods for measuring DNA repair rate in UV-irradiated bacteria. Immunochemistry13, 779–781 (1976)
Seaman, E., van Vunakis, H., Levine, L.: Serologic estimation of thymine dimers in the deoxyribonucleic acid of bacterial and mammalian cells following irradiation with ultraviolet light and postirradiation repair. J. Biol. Chem.247, 5709–5715 (1972)
Vielkind, J., Schwab, M., Anders, F.: Fate of bacterial DNA injected into embryos of poeciliid fish. In: Genetics and mutagenesis of fish. Schröder, H. J. (ed.). Berlin, Heidelberg, New York: Springer-Verlag 1973
Wassermann, E., Levine, L.: Quantitative microcomplement fixation and its use in the study of antigenic structure by specific antigenic and antibody inhibition. J. Immunol.87, 290–295 (1960)
Author information
Authors and Affiliations
Additional information
This work is part of the Ph.D. theses of H. Waller and E. Friess (D26, Justus-Liebig-Universität Gießen, FRG)
Rights and permissions
About this article
Cite this article
Waller, H., Friess, E. & Kiefer, J. On the immunological detection of X-ray induced DNA damage. Radiat Environ Biophys 19, 259–264 (1981). https://doi.org/10.1007/BF01324091
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01324091