Summary
We have successfully localized fungal actin for the first time using immuno-electron microscopy and hyphal tips of the rice blast pathogenMagnaporthe grisea. Following ultrarapid freezing, samples were processed in a novel substitution fluid of 10% acrolein in anhydrous ethanol and embedded in LR White resin. A monoclonal anti-actin antibody, previously shown to recognizeM. grisea actin, bound specifically to filasomes concentrated in the peripheral cytoplasm of subapical regions, and to the core-region of the Spitzenkörper.
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Abbreviations
- IEM:
-
immuno-electron microscopy
- TEM:
-
transmission electron microscopy
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Bourett, T.M., Howard, R.J. Ultrastructural immunolocalization of actin in a fungus. Protoplasma 163, 199–202 (1991). https://doi.org/10.1007/BF01323344
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DOI: https://doi.org/10.1007/BF01323344