Summary
The sequence of ORFs 2 to 4 of a U.S. isolate of porcine reproductive and respiratory syndrome virus (PRRSV), ATCC VR2385, was determined by analysis of a cDNA λ library. The cDNA clones containing PRRSV specific sequences were selected using a VR2385 ORF 4 specific PCR probe and sequenced. The ORFs 2, 3 and 4 overlapped each other and encoded polypeptides with predicted M r of 29.5 kDa (ORF 2), 28.7 kDa (ORF 3) and 19.5 kDa (ORF 4), respectively. No overlap was found between ORFs 4 and 5, and instead there was a 10 bp sequence which separated these two ORFs. The nucleic acid homology with corresponding ORFs of the European PRRSV isolate Lelystad virus (LV) was 65% for ORF 2, 64% for ORF 3 and 66% for ORF 4. Comparison of the ORF 4 sequences of VR2385 with that of another U.S. isolate MN-1b revealed only 86% amino acid sequence homology and the presence of deletions in the ORF 4 of MN-1b. Our results further strengthen the observation that there is sequence variation between US and European PRRSV isolates.
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Bautista EM, Goyal SM, Yoon IJ, Joo HS, Collins JE (1993). Comparison of porcine alveolar macrophages and CL 2621 for the detection of porcine reproductive and respiratory syndrome (PRRS) virus and anti-PRRS antibody. J Vet Diagn Invest 5: 163–165
Benfield DA, Nelson E, Collins JE, Harris L, Goyal SM, Robison D, Christianson WT, Morrison RB, Gorcyca D, Chladek D (1992) Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332). J Vet Diagn Invest 5: 612–614
Chen Z, Kuo L, Rowland RRR, Even C, Faaberg KS, Plagemann PGW (1993) Sequences of 3′ end of genome and of 5′ end of open reading frame la of lactate dehydrogenase-elevating virus and common junction motifs between 5′ leader and bodies of seven subgenomic mRNAs. J Gen Virol 74: 643–660
Collins JE, Benfield DA, Christianson WT, Harris L, Hennings JC, Shaw DP, Goyal SM, McCullough S, Morrison RB, Joo HS, Gorcyca D, Chladek D (1992) Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs. J Vet Diagn Invest 14: 117–126
Conzelmann K-K, Visser N, Van Woensel P, Thiel H-J (1993) Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the Arterivirus group. Virology 193: 329–339
Den Boon JA, Snijder EJ, Chirnside ED, De Vries AAF, Horzinek MC, Spaan WJM (1991) Equine arteritis virus is not a togavirus but belongs to the coronavirus-like superfamily. J Virol 65: 2910–2920
de Vries AAF, Chirnside ED, Horzinek MC, Rottier PJM (1992) Structural proteins of equine arteritis virus. J Virol 66: 6294–6303
Godeny EK, Chen L, Kumar SN, Methven SL, Koonin EV, Brinton MA (1993) Complete genomic sequence and phylogenetic analysis of the lactate dehydrogenase-elevating virus (LDV). Virology 194: 585–596
Hill H (1990) Overview and history of mystery swine disease (swine infertility and respiratory syndrome). In: Proceedings of the Mystery Swine Disease Committee Meeting, Denver, Colorado, pp 29–31. Denver, Livestock Conservation Institute
Kwang J, Kim HS, Joo HS (1994) Cloning, expression, and sequence analysis of the ORF4 gene of porcine reproductive and respiratory syndrome virus MN-1b. J Vet Diagn Invest 6: 293–296
Mardassi H, Mounir S, Dea S (1994) Identification of major differences in the nucleocapsid protein genes of a Quebec strain and European strains of porcine reproductive and respiratory syndrome virus. J Gen Virol 75: 681–685
Meng X-J, Paul PS, Halbur PG (1994) Molecular cloning and nucleotide sequencing of the 3′-terminal genomic RNA of porcine reproductive and respiratory syndrome virus. J Gen Virol 75: 1795–1801
Meng X-J, Paul PS, Halbur PG, Lum MA (1995) Phylogenetic analysis of the putative M (ORF 6) and N(ORF 7) genes of porcine reproductive and respiratory syndrome virus (PRRSV): implication for the existence of two genotypes of PRRSV in U.S. and Europe. Arch Virol 140: 745–755
Meulenberg JJM, Hulst MM, De Meijer EJ, Moonen PLJM, Den Besten A, De Kluyver EP, Wensvoort G, Moormann RJM (1993a) Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology 192: 62–72
Meulenberg JJM, De Meijer EJ, Moormann RJM (1993b) Subgenomic RNAs of Lelystad virus contain a conserved leader-body junction sequence. J Gen Virol 74: 1697–1701
Nelson EA, Christopher-Hennings J, Drew T, Wensvoort G, Collins JE, Benfield DA (1993) Differentiation of U.S. and European isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies. J Clin Microbiol 31: 3184–3189
Paul PS, Halbur PG, Meng X-J (1993) Porcine reproductive and respiratory syndrome: an overview. J Clin Vet Med 11: 19–28
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467
Terpstra C, Wensvoort G, Pol JMA (1991) Experimental reproduction of porcine epidemic abortion and respiratory syndrome (mystery swine disease) by infection with Lelystad virus: Koch's postulates fulfilled. Vet Q 13: 131–136
Wensvoort G, De Kluyver EP, Luijtze EA, Den Besten A, Harris L, Collins JE, Christinanson WT, Chladek D (1992) Antigenic comparison of Lelystad virus and swine infertility and respiratory syndrome virus. J Vet Diagn Invest 4: 134–138
Wensvoort G, Terpstra C, Pol JMA, Ter Laak EA, Bloemraad M, de Kluyver EP, Kragten C, van Buiten L, den Besten A, Wagenaar F, Broekhuijsen JM, Moonen PLJM, Zetstra T, de Boer EA, Tibben HJ, de Jong MF, Van't Veld P, Groenland GJR, van Gennep JA, Votes MT, Verheijden JHM, Braamskamp J (1991) Mystery swine disease in the Netherlands: the isolation of Lelystad virus. Vet Q 13: 121–130
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Morozov, I., Meng, X.J. & Paul, P.S. Sequence analysis of open reading frames (ORFs) 2 to 4 of a U.S. isolate of porcine reproductive and respiratory syndrome virus. Archives of Virology 140, 1313–1319 (1995). https://doi.org/10.1007/BF01322758
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DOI: https://doi.org/10.1007/BF01322758