Summary
Three monoclonal antibodies directed against cell surface molecules of bovine cells inhibited subsequent infections with bovine viral diarrhoea virus (BVDV). They specifically blocked the infectivity of three non-cytopathogenic and three cytopathogenic BVDV strains. These results showed that an important mechanism for virus uptake was inhibited. The ligand of the monoclonal antibody BVD/CA 17, which blocked infectivity most efficiently, was found on leukocytes from a wide range of domestic and wild even-toed ungulates using flow cytometric analysis. In contrast, the monoclonal antibodies BVD/CA 26 and BVD/CA 27 appeared to be specific for bovine cells. Immunoprecipitation of labelled bovine cell surface proteins showed that the three monoclonal antibodies bound to proteins with identical relative molecular masses (Mr). Proteins of an apparent Mr of 93 K and 60 K were precipitated from lysates of fetal bovine kidney cells irrespectively of the MAbs used.
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Allan GM, McNulty MS, Bryson D, Mackie D, Platten M (1989) Demonstration of bovine viral diarrhoea virus antigen in formalin fixed, paraffin embedded tissue using a streptavidin/biotin technique. Res Vet Sci 46: 416–418
Baker J (1987) Bovine viral diarrhoea virus: a review. J Am Vet Med Assoc 190: 242–261
Bielefeldt Ohmann H (1982) Experimental fetal infection with bovine viral diarrhoea virus. II. Morphological reactions and distribution of viral antigen. Can J Com Med 46: 363–369
Bielefeldt Ohmann H (1983) Pathogenesis of bovine viral diarrhoea mucosal disease: distribution and significance of BVDV antigen in diseased calves. Res Vet Sci 34: 5–10
Bielefeldt Ohmann H, Rønsholt L, Bloch B (1987) Demonstration of bovine viral diarrhoea virus in peripheral blood mononuclear cells of persistently infected, clinically normal cattle. J Gen Virol 68: 1971–1982
Bolin SR, McClurkin AW, Coria MF (1985) Effects of bovine viral diarrhoea virus on the percentages and absolute numbers of circulating B and T lymphocytes in cattle. Am J Vet Res 46: 884–886
Bolin S, Moennig V, Kelso Gourley NE, Ridpath J (1988) Monoclonal antibodies with neutralizing activity segregate isolates of bovine viral diarrhoea virus into groups. Arch Virol 99: 117–123
Boulanger D, Waxweiler S, Karelle L, Loncar M, Mignon B, Duduisson J, Thiry E, Pastoret PP (1991) Characterization of monoclonal antibodies to bovine viral diarrhoea virus: evidence of a neutralizing activity against gp 48 in the presence of goat anti-mouse immunoglobulin serum. J Gen Virol 72: 1195–1198
Citovsky V, Loyter A (1985) Fusion of Sendai virus or reconstituted Sendai virus envelopes with liposomes or erythrocyte membranes lacking virus receptors. J Biol Chem 260: 12702–12077
Corapi WV, French TW, Dubovi EJ (1989) Severe thrombocytopenia in young calves experimentally infected with noncytopathic bovine viral diarrhoea virus. J Virol 63: 3934–3943
Donis RO, Corapi W, Dubovi EJ (1988) Neutralizing monoclonal antibodies to bovine viral diarrhoea virus bind to the 56 K to 58 K glycoprotein. J Gen Virol 69: 77–86
Ellis JA, Davis WC, Belden EL, Pratt DL (1988) Flow cytofluorimetric analysis of lymphocyte subset alterations in cattle infected with bovine viral diarrhoea virus. Vet Pathol 25: 231–236
Greiser-Wilke I, Liebler E, Haas L, Liess B, Pohlenz J, Moenning V (1993) Distribution of cytopathogenic and noncytopathogenic bovine viral diarrhoea virus in tissues from a calf with experimentally induced mucosal disease using antigenic and genetic markers. In: Kaaden OR, Eichhorn W, Czerny CP (eds) Unconventional agents and unclassified viruses. Recent advances in biology and epidemiology. Springer, Wien New York, pp 295–301 (Arch Virol [Suppl] 7)
Greiser-Wilke I, Moenning V, Coulibaly COZ, Dahle J, Liess B (1990) Identification of conserved epitopes on a hog cholera virus protein. Arch Virol 111: 213–225
Greiser-Wilke I, Soine C, Moenning V (1989) Monoclonal antibodies reacting specifically withFrancisella sp. J Vet Med B 36: 593–600
Haywood AM (1994) Virus receptors: binding, adhesion strengthening, and changes in viral structure. J Virol 68: 1–5
Hewicker M, Wöhrmann T, Fernandez A, Trautwein G, Liess B, Moenning V (1990) Immunhistological detection of bovine viral diarrhoea virus antigen in the central nervous system of persistently infected cattle using monoclonal antibodies. Vet Microbiol 23: 203–210
Horzinek MC (1981) Non-arthropod-borne togaviruses. Academic Press, New York, London
Hyera JMK, Dahle J, Liess B, Moenning V, Frey HR (1987) Production of potent antisera raised in pigs by anamnestic response and use for direct immunofluorescent and immunoperoxidase techniques. In: Harkness JW (ed) Pestivirus infections of ruminants. Bruessels, Commission of the European Communities, publication CD-NA-10238-EN-C, pp 87–102
Laemmli UK (1979) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Lentz TL (1990) The recognition event between virus and host cell receptor: a target for antiviral agents. J Gen Virol 71: 751–766
Liess B, Moenning V (1990) Ruminant pestiviruses infection in pigs. Rev Sci Tech Off Int Epiz 9: 151–161
Lopez OJ, Osorio FA, Kelling CL, Donis RO (1994) Presence of bovine viral diarrhoea virus in lymphoid cell populations of persistently infected cattle. J Gen Virol 74: 925–929
Markwell MAK, Portner A, Schwartz AL (1985) An alternative route of infection of viruses; entry by means of the asialglycoprotein receptor of a Sendai virus mutant lacking its attachment protein. Proc Natl Acad Sci USA 82: 978–982
McClurkin AW, Bolin SR, Coria MF (1985) Isolation of cytopathic and noncytopathic bovine viral diarrhoea virus from the spleen of cattle acutely and chronically affected with bovine viral diarrhoea virus. J Am Vet Med Assoc 186: 568–569
Moenning V (1990) Pestiviruses: a review. Vet Microbiol 23: 35–54
Moenning V, Bolin RS, Mateo A, Kelso-Gourley NE, Wilke I, Liess B (1988) Identification of a single receptor for bovine viral diarrhoea virus (BVDV) on the surface of cultured cells. In: Abstracts of the annual meeting of the American Society for Microbiology, Miami Beach, Florida, 8–13 May, p. 326
Moenning V, Plagemann PG (1992) The pestiviruses. Adv Virus Res 41: 53–98
Nettleton PF (1990) Pestivirus infections in ruminants other than cattle. Rev Sci Tech Off Int Epiz 9: 131–150
Orban S, Liess B, Hafez SM, Frey HR, Blindow H, Sasse-Patzer B (1983) Studies on transplacental transmissibility of a bovine virus diarrhoea (BVD) vaccine virus. I. Inoculation of pregnant cows 15 to 90 days before parturition (190th to 265th day of gestation). J Vet Med B 30: 619–634
Peters W, Greiser-Wilke I, Moenning V, Liess B (1986) Preliminary serological characterization of bovine viral diarrhoea virus strains using monoclonal antibodies. Vet Microbiol 12: 195–200
Potts BJ, Sawyer M, Shekarchi IC, Wismer T, Huddleston D (1989) Peroxidase-labeled primary antibody method for detection of pestivirus contamination in cell cultures. J Virol Methods 26: 119–124
Reagan KJ, Goldberg B, Crowell RL (1984) Altered receptor specificity of coxsackievirus B3 after growth in rhabdomyosarcoma cells. J Virol 49: 635–640
Rümenapf T, Unger G, Strauss JH, Thiel HJ (1993) Processing of the envelope glycoproteins of pestiviruses. J Virol 67: 3288–3294
Sopp P, Hooper LB, Clarke MC, Howard CJ, Brownlie J (1994) Detection of bovine viral diarrhoea virus p80 protein in subpopulations of bovine leukocytes. J Gen Virol 75: 1189–1194
Von Boxberg Y, Wütz R, Schwarz U (1990) The use of the biotin-avidin system for labelling, isolation and characterization of neural cell-surface proteins. Eur J Biochem 190: 249–256
Weiland E, Stark R, Haas B, Rümenapf T, Meyers G, Thiel HJ (1990) Pestivirus glycoprotein which induces neutralizing antibodies forms part of a disulfide-linked heterodimer. J Virol 64: 3563–3569
Xue W, Minocha HC (1993) Identification of the cell surface receptor for bovine viral diarrhoea virus by using anti-idiotypic antibodies. J Gen Virol 74: 73–79
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Schelp, C., Greiser-Wilke, I., Wolf, G. et al. Identification of cell membrane proteins linked to susceptibility to bovine viral diarrhoea virus infection. Archives of Virology 140, 1997–2009 (1995). https://doi.org/10.1007/BF01322688
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DOI: https://doi.org/10.1007/BF01322688