Summary
Cytokinesis in microsporocytes of moth orchids is unusual in that it occurs simultaneously after meiosis, the cytoplasm does not infurrow in the division planes, and cell plates are deposited in association with centrifugal expansion of phragmoplasts. Microtubules radiating from the nuclear envelopes appear to be of fundamental importance in establishment of division planes. Primary interzonal spindles develop between sister nuclei and interaction of radial microtubules triggers development of secondary interzonal spindles between non-sister nuclei. From three to six or more phragmoplasts, depending upon the arrangement of nuclei in the coenocyte, develop from these postmeiotic arrays. The phragmoplasts consist of co-aligned microtubules and F-actin organized into bundles that are broad proximal to the mid-plane and taper distally. Ultrastructure of the phragmoplast/cell plate reveals that abundant ER is associated with vesicle aggregation and coalescence. Cell plates are deposited in association with phragmoplasts as they expand centrifugally to join the parental wall and/or fuse with one another in the interior of the cell.
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Abbreviations
- CLSM:
-
confocal laser scanning microscope/microscopy
- FITC:
-
flnorescein isothiocyanate
- PPB:
-
preprophase band of microtubules
- TEM:
-
transmission electron microscope/microscopy
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Brown, R.C., Lemmon, B.E. Pollen development in orchids. Protoplasma 165, 155–166 (1991). https://doi.org/10.1007/BF01322286
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DOI: https://doi.org/10.1007/BF01322286