Summary
The appearence of cell-mediated immunity was studied in Aujeszky diseased pigs with the aid of thein vitro stimulation of sensitized lymphocytes. The first cell-mediated immunity reaction of lymphocytes occurred 4 days after infection. From day 7 to 35, the latest day tested, the reactions were most marked with lymphocytes from lymph nodes and spleen, whereas blood and thymus lymphocytes reacted less frequently; bone marrow lymphocytes showed no response. Reinfection did not considerably enhance lymphocyte reactivity. Humoral immunity was demonstrated a few days later than cell-mediated immunity. Neutralizing antibodies were first detected at day 7, reaching optimal titers at day 14. Complement fixing antibodies were detected from day 14 onward. Reinfection caused a very weak booster effect only on neutralizing antibody production. The sensitivity of the neutralization test could be enhanced up to sixfold by the addition of fresh guinea pig complement.
It is concluded that cell-mediated immunity influences the early stage of infection with Aujeszky disease virus when humoral immunity is not yet demonstrable or yet rather low. Lymph nodes and spleen are apparently of special importance for the appearance of ADV-reactive lymphocytes.
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Abbreviations
- ADV:
-
Aujeszky disease
- CMI:
-
cell-mediated immunity
- FCS:
-
fetal calf serum
- MEM:
-
Eagle's minimal essential medium
- PBS:
-
phosphate buffered saline
- SCP:
-
single cell protein
References
Boyle, W.: An extension of the51Cr-release assay for the estimation of mouse cytotoxins. Transplantation6, 761–764 (1968).
Davies, D. H., Carmichel, L. E.: Role of cell-mediated immunity in the recovery of cattle from primary and recurrent infections with infectious bovine rhinotracheitis virus. Infect. Immun.7, 510–517 (1973).
Kärber, G.: Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak.162, 480–483 (1931).
Lausch, R. N., Swyers, J. S., Kaufman, H. E.: Delayed hypersensitivity to herpes simplex virus in the guinea pig. J. Immunol.96, 981–987 (1966).
Meyers, R. L., Pettit, T. H.: Corneal immune response to herpes simplex virus antigen. J. Immunol.110, 1575–1590 (1973).
Rogers, H. W., Scott, V., Patnode, R. A.: Sensitization of guinea pigs to herpes simplex virus. J. Immunol.109, 801–906 (1972).
Rosenberg, G. L., Farber, P. A., Notkins, A. L.:In vitro stimulation of sensitized lymphocytes by herpes simplex virus and vaccinia virus. Proc. Nat. Acad. Sci. U.S.A.69, 756–760 (1972).
Rosenberg, G. L., Notkins, A. L.: Induction of cellular immunity to herpes simplex virus: relationship to the humoral immune response. J. Immunol.112, 1019–1025 (1974).
Rouse, B. T., Babiuk, L. A.: Host defense mechanisms against infectious bovine rhinotracheitis virus:in vitro stimulation of sensitized lymphocytes by virus antigen. Infect. Immun.10, 681–687 (1974).
Rouse, B. T., Babiuk, L. A.: Host responses to infectious bovine rhinotracheitis virus. III. Isolation and immunologic activity of bovine Tlymphocytes. J. Immunol.113, 1391–1398 (1974).
Russell, A. S.: Cell-mediated immunity to herpes simplex virus in man. J. inf. Dis.129, 142–146 (1974).
Schwöbel, W., Ahl, R.: Persistance of Sindbis virus in BHK-21 cell-cultures. Arch. ges. Virusforsch.38, 1–10 (1972).
Scriba, M.: Stimulation of peripheral blood lymphocytes by herpes simplex virusin vitro. Infect. Immun.10, 430–436 (1974).
Skoda, R.: Der Nachweis von neutralisierenden Antikörpern gegen das Virus der Aujeszkyschen Krankheit bei Schweinen in Süddeutschland. Berl. Münch. tierärztl. Wschr.84, 411–414 (1971).
Skoda, R., Ivanicova, St., Jarmichova, O., Sliepka, M.: Die kutane Überempfindlichkeit der Schweine bei Aujeszkyscher Krankheit. Arch. exp. vet. Med.22, 925–936 (1968).
Skoda, R., Jakubik, J.: Komplementbindungsreaktion bei der Aujeszkyschen Krankheit. Berl. Münch. tierärztl. Wschr.20, 387–392 (1972).
Skoda, R., Wittmann, G.: Die Immunisierung von Schweinen mit Vakzinen aus inaktiviertem Aujeszky-Virus. Zbl. Vet. Med. B,20, 127–138 (1973).
Wolstencroft, R. A., Dumonde, D. C.: The demonstration of lymphocyte mitogen factor in the guinea pig. In:Bloom, B. R., Glade, P. R. (eds.),In vitro Methods in Cell-mediated Immunity. (Conf. on ‘In vitro Methods in Cell-mediated Immunity’, New York, 1970) 423–432. New York-London: Academic Press 1971.
Yoshino, K., Tanaguchi, S.: Studies on the neutralization of herpes simplex virus. I. Appearance of neutralizing antibodies having different grades of complement requirement. Virology26, 44–53 (1965).
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This investigation was done as a part of the research program of the Ministry of Agriculture entitled ‘Improvement of the efficiency of agriculture and food economy by a new technology of milk extraction, harmonizing the expense of food with animal productivity, and the use of biosynthetic protein, made from by-products, for feeding’.
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Wittmann, G., Bartenbach, G. & Jakubik, J. Cell-mediated immunity in Aujeszky disease virus infected pigs. Archives of Virology 50, 215–222 (1976). https://doi.org/10.1007/BF01320575
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DOI: https://doi.org/10.1007/BF01320575