Summary
Purified viral preparations of influenza A virus were examined for the presence of NS2 protein hitherto considered as a viral nonstructural protein that is present only in infected cells. Analysis of purified virus by radioimmunoprecipitation with monospecific antisera to NS2 revealed its presence in the virus particle suggesting that it is a viral structural protein. NS2 protein was also shown to be phosphorylated in infected cells in this study. This brings the number of influenza virus phosphoproteins to three which include NP, NS1, and NS2. These observations raise important questions about the role of NS2 in the replication of influenza virus.
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References
Akkina RK, Nayak DP (1987) Interference by defective interfering influenza virus: role of defective viral polypeptides in infected cells. In: Mahy BWJ, Kolakofsky D (eds) The biology of negative strand viruses. Elsevier, New York, pp 183–190
Akkina RK, Chambers TM, Nayak DP (1984) Expression of defective-interfering influenza virus-specific transcripts and polypeptides in infected cells. J Virol 51: 395–403
Akkina RK, Chambers TM, Londo DR, Nayak DP (1987) Intracellular localization of the viral polymerase proteins in cells infected with influenza virus and cells expressing PB1 protein from cloned cDNA. J Virol 61: 2217–2224
Banerjee AK (1987) Transcription and replication of rhabdoviruses. Microbiol Rev 51: 66–87
Barret T, Inglis SC (1985) Growth, purification, and titration of influenza viruses. In: Mahy BWJ (ed) Virology: a practical approach. IRL Press, Oxford, pp 119–150
Greenspan D, Krystal M, Nakada S, Arnheiter H, Lyles DS, Palese P (1985) Expression on influenza virus NS2 nonstructural protein in bacteria and localization of NS2 in infected eukaryotic cells. J Virol 54: 833–843
Inglis SC, Gething MJ, Brown CM (1980) Relationship between the messenger RNAs transcribed from two overlapping genes of influenza virus. Nucleic Acids Res 8: 3575–3589
Koennecke I, Boschek CB, Scholtissek C (1981) Isolation and properties of a temperature-sensitive mutant (ts 412) of the influenza A virus recombinant with a ts lesion in the gene coding for nonstructural protein. Virology 110: 16–25
Lamb RA, Choppin PW (1976) Synthesis of influenza virus proteins in infected cells: translation of viral polypeptides, including three P polypeptides, from RNA produced by primary transcription. Virology 74: 504–519
Lamb RA, Choppin PW (1979) Segment 8 of the influenza virus genome is unique in coding for two polypeptides. Proc Natl Acad Sci USA 46: 4908–4912
Lamb RA, Lai CJ (1980) Sequence of interrupted and uninterrupted mRNAs and cloned DNA coding for the two overlapping nonstructural proteins of influenza virus. Cell 21: 475–485
Lamb RA, Choppin PW (1983) The gene structure and replication of influenza virus. Annu Rev Biochem 52: 467–506
Lamb RA, Etkind PR, Choppin PW (1978) Evidence for a ninth influenza viral polypeptide. Virology 91: 60–78
Privalsky ML, Penhoet EE (1981) The structure and synthesis of influenza virus phosphoproteins. J Biol Chem 256: 5368–5376
Shimizu K, Mullinix MG, Chanock RM, Murphy BR (1982) Temperature sensitive mutants of influenza A/Udorn/72 (H3N2) virus. I. Isolation of temperature sensitive mutants some of which exhibit host dependent temperature sensitivity. Virology 117: 38–44
Smith GL, Levin JZ, Palese P, Moss E (1987) Synthesis and cellular location of the ten influenza polypeptides individually expressed by recombinant vaccinia virus. Virology 160: 336–345
Wolstenholme AJ, Barrett T, Nichol ST, Mahy BWJ (1980) Influenza virus specific RNA and protein synthesis in cells infected with temperature sensitive-mutants defective in the genome segment encoding nonstructural proteins. J Virol 35: 1–7
Young JF, Desselberger U, Palese P, Ferguson B, Shatzman AR, Rosenberg M (1983) Efficient expression of influenza virus NS1 nonstructural proteins inEscherichia coli. Proc Natl Acad Sci USA 80: 6105–6109
Zebedee SL, Lamb RA (1988) Influenza A virus M2 protein: monoclonal antibody restriction of virus growth and detection of M2 in virions. J Virol 62: 2762–2772
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Richardson, J.C., Akkina, R.K. NS2 protein of influenza virus is found in purified virus and phosphorylated in infected cells. Archives of Virology 116, 69–80 (1991). https://doi.org/10.1007/BF01319232
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DOI: https://doi.org/10.1007/BF01319232