Summary
A virus morphologically resembling members of the family Paramyxoviridae has been isolated from the brain of a piglet with a central nervous disorder accompanied by pneumonia and corneal opacity. The virus, designated LPM, grows in a large variety of cultured cell types and elicits a cytopathic effect including formation of syncytia and cytoplasmic inclusion bodies. The virus has hemagglutinating, neuraminidase and hemolytic activities. Studies on experimental transmission showed that young pigs are susceptible to infection which induced a disease similar to that in natural cases. The virus killed mice and chicken embryos. The structural proteins of LPM virus, as resolved by polyacrylamide gel electrophoresis are similar to those described for other paramyxoviruses. Serologically the virus proved to be distinct from the paramyxoviruses tested so far.
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References
Andrewes C, Pereira HG, Wildy P (1978) Viruses of vertebrates, 4th edn. Bailliere Tindall, London, pp 122–254
Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindning. Analyt Biochem 72: 248–254
Dinter Z, Hermodsson S, Hermodsson L (1964) Studies on myxovirus Yucaipa: Its classification as a member of the paramyxovirus group. Virology 22: 297–304
Jungherr EL (1974) Pathogenicity of Newcastle disease virus for the chicken. In:Hanson RP (ed) Newcastle disease virus, an evolving pathogen. University of Wisconsin Press, Madison, Wisc, pp 257–272
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Luther P, Adamczyk B, Bergmann KC (1980) Simple test for detection of virus neuraminidase and anti-neuraminidase using lectins (lectin-neuraminidase-test system). Zbl Bakt Hyg I Abt Orig A 248: 281–285
McSharry J, Benzinger R (1970) Concentration and purification of Vesicular stomatitis virus by polyethylene glycol “precipitation”. Virology 40: 745–779
Stephano H, Gay M, Ramirez C, Maqueda J (1981) An outbreak of encephalitis in piglets produced by an hemagglutinating virus. Proc of the international pig veterinary society congress, Mexico, p 153
Stephano H, Gay M (1984) Experimental studies on a viral syndrome in pigs called “Blue Eye”, characterized by encephalitis and corneal opacity. Proc of the International pig veterinary society, 8th congress, Ghent, Belgium, chap II, p 71
Wadsworth C (1957) A slide microtechnique for the analysis of immune precipitates in gel. Int Arch Allergy 10: 355–360
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Moreno-López, J., Correa-Girón, P., Martinez, A. et al. Characterization of a paramyxovirus isolated from the brain of a piglet in Mexico. Archives of Virology 91, 221–231 (1986). https://doi.org/10.1007/BF01314282
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DOI: https://doi.org/10.1007/BF01314282