Summary
The effects of human α-, β-, or γ-interferon (IFN) on the replication and production of human T-lymphotrophic virus type-I (HTLV-I) were investigated in a human T-cell line, MT-2. Virus transmission and production estimated by syncytium formation and HTLV-I-associated reverse transcriptase (RT) activity were strongly suppressed in the presence of α- and β-IFN, but not γ-IFN. However, the expression of virus specific proteins gp46 but not p19, p24, p28, p36, and gp68 was affected with IFNs as revealed by Western blotting analysis. Electron microscopic observations showed that some of the HTLV-I particles were trapped in the intracellular vacuoles in the presence of high doses of α- or β-IFN. Continuous supply of IFNs appeared to be essential for the constant suppression of RT activity. These results suggest that α- and β-IFN do not inhibit HTLV-I gene expression strikingly but suppress processing or assembly of virus proteins and/or releasing of virions in the late phase of maturation.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Isaacs A, Lindemann J (1957) Virus interference. I. The inferferon. Proc R Soc Lond [Biol] 147: 258–267
Taniguchi T, Fujii-Kuriyama Y, Muramatsu M (1980) Molecular cloning of human interferon cDNA. Proc Natl Acad Sci USA 77: 4003–4006
Balkwill FR. Taylor-Papadimitriou J (1978) Interferone, affects both G1 and S+G2 in cells stimulated from quiescence to growth. Nature 274: 798–800
Morris A, Tomkins, PT, Maudsley DJ, Blackman M (1987) Infection of cultured murine brain cells by Semliki Forest virus: effects of interferon-alpha, beta on viral replication, viral antigen display, major histocompatibility complex antigen display and lysis by cytotoxic T lymphocytes. J Gen Virol 68: 99–106
Gastl G, Huber C (1988) The biology of interferon actions. Blut 56: 193–199
Hoshino H, Shimoyama M, Miwa M, Sugimura T (1983) Detection of lymphocytes producing a human retrovirus associated with adult T-cell leukemia by syncytia induction assay. Proc Natl Acad Sci USA 80: 7337–7341
Osame M, Usuku K, Izumo S, Ijichi N, Amitani H, Igata A, Matsumoto M, Tara M (1986) HTLV-I associated myelopathy, a new clinical entity. Lancet i: 1031–1032
Popovic M, Flomenberg N, Volkman DJ, Mann D, Fauci AS, Dupont B, Gallo RC (1984) Alteration of T cell functions by infection with HTLV-I or HTLV-II. Science 226: 459
Salahuddin SZ, Markham PD, Wang-Staal F, Franchini GA, Kalyanaraman VS, Gallo RC (1983) Restricted expression of Human T-cell leukemia-lymphoma virus (HTLV) in transformed human umbilical cord blood lymphocytes. Virology 129: 51–64
Miyoshi I, Kubonishi I, Yoshimoto S, Akagi T, Ohtsuki Y, Shiraishi Y, Nagata K, Hinuma Y (1981) Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord lymphocytes and human leukemic T cells. Nature 294: 770–771
Ohtsuki Y, Miyoshi I, Oka T, Hayashi K, Takahashi K, Furihata M, Iwata J, Takeuchi T, Sonobe H (1988) Immunohistochemical and ultrastructural characterization of human T-lymphotropic virus type-I (HTLV-I)-producing rabbit lymphoid cell lines. Arch Virol 100: 245–254
Miyoshi I, Hiraki S, Tsubota T, Kubonishi I, Matsuda I, Nakayama T, Kishimoto H, Kimura I (1977). Human B cell, T cell and null cell leukemic cell lines derived from acute lymphoblastic leukemia. Nature 267: 843–844
Fischinger PJ, Peebles PR, Nomura S, Haapala DK (1973) Isolation of an RD-114-like oncornavirus from cat cell line. J Virol 11: 978–985
Ohtsuki Y, Akagi T, Takahashi K, Miyoshi I (1982) Ultrastructural study on type C virus particles in a human cord T-cell line established by co-cultivation with adult T-cell leukemia cells. Arch Virol 73: 69–73
Towbin H, Staehlin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellurose sheets; procedures and some applications. Proc Natl Acad Sci USA 76: 4350–4354
Rho HM, Poiesz B, Ruscetti FW, Gallo RC (1981) Characterization of the reverse transcriptase from a new retrovirus (HTLV) produced by a human cutaneous T-cell lymphoma cell line. Virology 112: 355–360
D'Onofrio C, Perno CF, Mazzetti P, Graziani G, Calio R, Bonmassar E (1988) Depression of early phase of HTLV-I infection in vitro mediated by human beta-interferon. Br J Cancer 57: 481–488
Billiau A, Edy VG, Sobis H, De Somer P (1974) Influence of interferon on virus particle synthesis in oncornavirus carrier. Evidence for a direct effect on particle release. Int J Cancer 14: 335–340
Billiau A, Heremans H, Allen PT, De Maeyer-Guignard J, De Somer P (1976) Trapping of oncornavirus particles at the surface of interferon treated cells. Virology 73: 537–542
Friedman RM, Ramseur JM (1974) Inhibition of murine leukemia virus production in chronically infected AKR cells: a novel effect of interferon. Proc Natl Acad Sci USA 71: 3542–3544
Ohtsuki Y, Oka T, Furihata M, Takahashi K, Hayashi K, Iwata J, Hikita T, Sonobe H, Miyoshi I (1989) Electron microscopic study of suppressive effects on the production of human T-lymphotropic virus type-I (HTLV-I) with human interferons. J Clin Electron Microsc 22: 205–213
Miyoshi I, Fujishita M, Ohtsuki Y, Akagi T (1982) Suppression by interferon of human type C retrovirus production in a human T-cell line. Interferon Scientific Memoranda 6: 6
Nishida J, Yoshikura H, Okabe T, Urabe A, Takaku F (1985) Interferons inhibit syncytia forming ability and in vitro transmission of human T-cell leukemia virus. Jp J Cancer Res 76: 249–252
Saigo K, Shinozawa S, Wakuya J, Ueda K, Yamaguchi N, Shibata H, Masaoka T (1988) Alpha-interferon treatment for adult T-cell leukemia: low levels of circulating alpha-interferon and its clinical effectiveness. Blut 56: 83–86
Abb J, Zachoval R, Zachoval V, Deinhardt F (1987) HIV antigen, HIV antibody and serum interferon in a patient with encephalopathy. Infection 15: 425–426
Moore JL, Poiesz BJ, Zamkoff KW, Merl SA, Hanna S, Gazdar AF, Comis RL (1985) Inverse relationship between constitutive gamma-interferon production and human T-cell leukemia/lymphoma virus expression in cultured T lymphocytes. J Virol 53: 440–446
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Oka, T., Ohtsuki, Y., Sonobe, H. et al. Suppressive effects of interferons on the production and release of human T-lymphotropic virus type-I (HTLV-I). Archives of Virology 115, 63–73 (1990). https://doi.org/10.1007/BF01310623
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01310623