Summary
The nucleotide sequence of the gene encoding the fusion protein of phocine distemper virus has been determined. The mRNA is 2206 nucleotides in length and contains one major open reading frame (ORF) of 1893 nucleotides encoding a potential protein of 631 amino acid residues. However, analogy with canine distemper virus (CDV) suggests that translation of the F protein starts at the sixth AUG codon in the mRNA sequence which is located at position 461, resulting in an F0 protein of exactly the same size (537 aa) as that of CDV. The overall homology at nucleotide level between the CDV and PDV F genes is 66%. The homology between the two F proteins of these respective viruses is 83%.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Barrett T, Underwood B (1985) Comparison of messenger RNAs induced in cells infected with each member of the morbillivirus group. Virology 145: 195–199
Barrett T, Clarke DK, Evans SA, Rima BK (1987) The nucleotide sequence of the gene encoding the F protein of canine distemper virus: a comparison of the deduced amino acid sequence with other paramyxoviruses. Virus Res 8: 373–386
Barrett T, Subbarao SM, Belsham GJ, Mahy BWJ (1991) The molecular biology of the morbilliviruses. In: Kingsbury DW (ed) The paramyxoviruses. Plenum Press, New York, pp 83–102
Blumberg BM, Giorgi C, Rose K, Kolakofsky D (1985) Sequence determination of the Sendai virus fusion protein gene. J Gen Virol 66: 317–331
Buckland R, Wild TF (1989) Leucine zipper motif extends. Nature 338: 547
Campbell JJ, Cosby SL, Scott JK, Rima BK, Martin SJ, Appel M (1980) A comparison of measles and canine distemper virus polypeptides. J Gen Virol 48: 149–159
Cattaneo R, Kaelin K, Baczko K, Billeter MA (1989) Measles virus editing provides an additional cysteine rich protein. Cell 56: 759–764
Chirgwin JM, Przybyla AE, MacDonald R, Rutter WJ (1979) Isolation of biologically active ribonucleic acid from sources rich in ribonuclease. Biochemistry 18: 5294–5299
Cosby SL, McQuaid S, Duffy N, Lyons C, Rima BK, Allan GM, McCullough SJ, Kennedy S, Smyth JA, McNeilly F, Craig C, Örvell C (1988) Characterisation of a seal morbillivirus. Nature 336: 115–116
Curran MD, O'Loan D, Rima BK, Kennedy S (1990) Nucleotide sequence analysis of phocine distemper virus reveals its distinctness from canine distemper virus. Vet Rec 127: 430–431
Curran MD, O'Loan D, Kennedy S, Rima BK (1992) Molecular characterization of phocine distemper virus: gene order and sequence of the gene encoding the attachment (H) protein. J Gen Virol 73: 1189–1194
Evans SA, Belsham GJ, Barrett T (1990) The role of the 5′ nontranslated regions of the fusion protein mRNAs of canine distemper virus and rinderpest virus. Virology 177: 317–323
Feinberg AP, Vogelstein B (1984) A technique for radiolabelling DNA restriction fragments to high specific activity. Anal Biochem 137: 266–269
Gubler V, Hoffmann BJ (1983) A simple and very efficient method for generating cDNA libraries. Gene 25: 263–269
Hasel KW, Day S, Millward S, Richardson CD, Bellini WJ, Greer PA (1987) Characterization of cloned measles virus mRNAs by in vitro transcription, translation and immuno precipitation. Intervirology 28: 26–39
Hsu D, Yamanaka M, Miller J, Dale B, Grubman M, Yilma T (1988) Cloning of the fusion gene of rinderpest virus: comparative sequence analysis with other morbilliviruses. Virology 166: 149–153
Kennedy S, Smyth JA, McCullough SJ, Allan GM, McNeilly F, McQuaid S (1988) Confirmation of cause of recent seal deaths. Nature 335: 404
Kövamees J, Blixenkrone-Möller M., Sharma B, Örvell C, Norrby E (1991) The nucleolide sequence and deduced amino acid composition of the haemagglutinin and fusion proteins of the morbillivirus phocid distemper virus. J Gen Virol 72: 2959–2966
McCullough SJ, McNeilly F, Allan GM, Kennedy S, Smyth JA, Cosby SL, McQuaid S, Rima BK (1991) Isolation and characterisation of a porpoise morbillivirus. Arch Virol 118: 247–252
Örvell C, Blixenkrone-Möller M, Svansson V, Have P (1990) Immunological relationships between phocid and canine distemper virus studied with monoclonal antibodies. J Gen Virol 71: 2085–2092
Osterhaus ADME, Vedder EJ (1988) Identification of virus causing recent seal deaths. Nature 335: 20
Osterhaus ADME, Groen J, de Vries P, UytdeHaag FGCM, Klingeborn B, Zarnke R (1988) Canine distemper virus in seals. Nature 335: 403–404
Osterhaus ADME, Uytdehaag FGCM, Visser IKG, Vedder EJ, Reynders PJM, Kuiper J, Brugge HN (1989) Seal vaccination success. Nature 337: 21
Richardson CD, Hull D, Greer P, Hasel K, Berkovich A, Englund G, Bellini WJ, Rima BK, Lazzarini RA (1986) The nucleotide sequence of the mRNA encoding the fusion protein of measles virus (Edmonston strain): a comparison of fusion proteins from several different paramyxoviruses. Virology 155: 508–523
Rima BK (1989) Comparison of amino acid sequences of the major structural proteins of the paramyxo- and morbilliviruses. In: Kolakofsky D, Mahy BWJ (eds) Genetics and pathogenicity of negative strand viruses. Elsevier, Amsterdam, pp 254–263
Rima BK, Cosby SL, Duffy N, Lyons C, O'Loan D, Kennedy S, McCullough SJ, Smyth JA, McNeilly F (1990) Humoral immune responses in seals infected with phocine distemper virus. Res Vet Sci 149: 114–116
Tsukiyama K, Yoshikawa Y, Yamanouchi K (1988) Fusion glycoprotein (F) of rinderpest virus: entire nucleotide sequence of the F mRNA, and several features of the F protein. Virology 164: 523–530
Udem SA, Cook KA (1984) Isolation and characterisation of measles virus intracellular nucleocapsid RNA. J Virol 49: 57–65
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Curran, M.D., Lü, Y.J. & Rima, B.K. The fusion protein gene of phocine distemper virus: nucleotide and deduced amino acid sequences and a comparison of morbillivirus fusion proteins. Archives of Virology 126, 159–169 (1992). https://doi.org/10.1007/BF01309692
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01309692