Summary
The 3′-terminal 2,561 nucleotide residues of the severe HA strain of papaya ringspot virus (PRSV) was determined. Comparison with the published sequence of the mild strain PRSV HA 5-1 showed that they shared a 99.4% identity in their 3′-terminal 2,235 residues. There were ten residues different at the NIb gene, resulting in five amino acid changes, and two residues different in the coat protein gene, resulting in two amino acid changes. The 3′-untranslated regions were identical, but HA contained two more nucleotides (AG) at the 3′ extreme. Comparison with the published non-papaya infecting type W strain PRSV-W revealed that they shared a 97.9% identity in their 3′-terminal 2,235 residues. There were 40 nucleotides different in the coding region, which resulted in four amino acid changes in the NIb gene and six in the CP gene, and seven nucleotides different in the 3′-untranslated region.
Article PDF
Avoid common mistakes on your manuscript.
References
Allison R, Johnston RE, Dougherty WG (1986) The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154: 9–20
Atreya CD, Raccach B, Pirone TP (1990) A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus. Virology 178: 161–165
Carrington JC, Dougherty WG (1987) Processing of the tobacco etch virus 49 K protease requires autoproteolysis. Virology 160: 355–362
Carrington JC, Dougherty WG (1987) Small nuclear inclusion protein encoded by a plant potyvirus genome is a protease. J Virol 61: 2540–2548
Carrington JC, Cary SM, Parks TD, Dougherty WG (1989) A second proteinase encoded by a plant potyvirus. EMBO J 8: 365–370
Davis LG, Dibner MD, Battey JF (1986) Basic methods in molecular biology. Elsevier, New York
De La Rosa M, Lastra R (1983) Purification and partial characterization of papaya ringspot virus. Phytopathol Z 106: 329–336
Domier LL, Franklin KM, Shahabuddin M, Hellmann GM, Overmeyer JH, Hiremath ST, Siaw MFE, Lomonssoff GP, Shaw JG, Rhoads RE (1986) The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acids Res 14: 5417–5430
Dougherty WG, Carrington JC (1988) Expression and function of potyviral gene products. Annu Rev Phytopathol 26: 123–143
Dougherty WG, Cary SM, Parks TD (1989) Molecular genetic analyis of a plant virus polyprotein cleavage site: a model. Virology 171: 356–364
Gonsalves D, Ishii M (1980) Purification and serology of papaya ringspot virus. Phytopathology 70: 1028–1032
Gubler U, Hoffman BJ (1983) A simple and very efficient method for generating cDNA libraries. Gene 25: 263–269
Henikoff S (1984) Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28: 351–359
Huynh TV, Young RA, Davis RW (1985) Constructing and screening cDNA libraries in λgt 10 and λgt 11. In: Glover DM (ed) DNA cloning, a practical approach, vol 1. IRL Press, Oxford, pp 49–79
Johansen E, Rasmussen OF, Heide M, Borkhardt B (1991) The complete nucleotide sequence of pea seed-borne mosaic virus RNA. J Gen Virol 72: 2625–2632
Maiss E, Timpe U, Brisske A, Jelkmann W, Casper R, Himmler G, Mattanovich D, Katinger HWD (1989) The complete nucleotide sequence of plum pox virus RNA. J Gen Virol 70: 513–524
Martelli GP, Russo M (1976) Unusual cytoplasmic inclusions induced by watermelon mosaic virus. Virology 72: 352–362
Nagel J, Hiebert E (1985) Complementary DNA cloning and expressing of the papaya ringspot potyvirus sequences encoding capsid protein and a nuclear inclusion-like protein inEscherichia coli. Virology 143: 435–441
Provvidenti R, Gonsalves D (1982) Resistance to papaya ringspot virus inCucumis metuliferus and its relationship to resistance to watermelon mosaic virus 1. J Hered 73: 239–240
Purcifull DE, Edwardson JR (1967) Watermelon mosaic virus: tubular inclusion in pumpkin leaves and aggregates in leaf extracts. Virology 32: 393–401
Purcifull DE, Hiebert E (1979) Serological distinction of watermelon mosaic virus isolates. Phytopathology 69: 112–116
Purcifull DE, Edwardson JR, Hiebert E, Gonsalves D (1984) Papaya ringspot virus. CMI/AAB Descriptions of Plant Viruses, no 292
Quemada H, L'Hostis B, Gonsalves D, Reardon IM, Heinrikson R, Hiebert EL, Sieu LC, Slightom JL (1990) The nucleotide sequences of the 3′-terminal regions of papaya ringspot virus strains W and P. J Gen Virol 71: 203–210
Robaglia C, Durand-Tardif M, Tronchet M, Boudazin G, Astier-Manifacier S, Casse-Delbart F (1989) Nucleotide sequence of potato virus Y (N strain) genomic RNA. J Gen Virol 70: 935–947
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467
Short JM, Fernandez JM, Sorge JA, Huse WD (1988) Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties. Nucleic Acids Res 16: 7583–7600
Shukla DD, Strike PM, Tracy SL, Gough KH, Ward CW (1988) The N-and C-termini of the coat proteins of potyviruses are surface-located and the N-terminus containing the major virus-specific epitopes. J Gen Virol 69: 1497–1508
Wang HL, Yeh SD, Chiu RJ, Gonsalves D (1987) Effectiveness of cross-protection by mild mutants of papaya ringspot virus for control of ringpot disease of papaya in Taiwan. Plant Dis 71: 491–497
Yeh SD, Gonsalves D (1984) Evaluation of induced mutants of papaya ringspot virus for control by cross protection. Phytopathology 74: 1086–1091
Yeh SD, Gonsalves D (1984) Purification and immunological analyses of cylindrical inclusion protein induced by papaya ringspot virus and watermelon mosaic virus 1. Phytopathology 74: 1273–1278
Yeh SD, Gonsalves D (1985) Translation of papaya ringspot virus RNA in vitro: detection of a possible polyprotein that is processed for capsid protein, cylindrical-inclusion protein, and amorphous-inclusion protein. Virology 143: 260–271
Yeh SD, Gonsalves D, Provvidenti R (1984) Comparative studies on host range and serology of papaya ringspot virus and watermelon mosaic virus 1. Phytopathology 74: 1081–1085
Yeh SD, Gonsalves D, Wang HL, Namba R, Chiu RJ (1988) Control of papaya ringspot virus by cross protection. Plant Dis 72: 375–380
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wang, C.H., Yeh, S.D. Nucleotide sequence comparison of the 3′-terminal regions of severe, mild, and non-papaya infecting strains of papaya ringspot virus. Archives of Virology 127, 345–354 (1992). https://doi.org/10.1007/BF01309597
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01309597