Abstract
Effect of aging on the intestinal permeability to medium size [3H]polyethylene glycol (PEG 900) was examinedin vivo by gavage andin vitro in intestinal everted sacs of rats ranging in age from 5 to 102 weeks. Rats were gavaged with PEG 900 solution and urinary recovery of PEG 900 was measured for 6 hr in order to assess its absorption. Young rats, 5–15 weeks of age, absorbed 1–1.3% of administered PEG 900. In contrast, rats 35–102 weeks of age absorbed 1.8–2.4% of administered PEG 900 (P<0.05 vs younger animals). The increased absorption of PEG 900 with aging is due to changes in intestinal permeability since the total uptake (serosal appearance + tissue uptake) of PEG 900 by jejunum, ileum, and colonic everted sacs was significantly higher (P<0.05) in older rats (100 weeks) than young rats (9 1/2 weeks), while urinary excretion of PEG 900 following intravenous injection was the same in the two age groups. These studies indicate that aging rats have diminished capacity to exclude larger size molecules from penetrating the intestinal mucosa. The diminished barrier functions of the small intestine with aging may allow antigenic or mutagenic compounds to reach the systemic circulation.
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Chadwick VS, Phillips SF, Hofman AF: Measurements of intestinal permeability using low molecular weight polyethylene glycols (PEG 400). I. Chemical analysis and biological properties of PEG 400. Gastroenterology 73:241–246, 1977
Chadwick VS, Phillips SF, Hofman AF: Measurments of intestinal permeability using low molecular weight polyethylene glycols (PEG 400). II. Application to study of normal and abnormal permeability stated in man and animals. Gastroenterology 73:247–251, 1977
Tagesson C, Sjodahl R: Passage of molecules through the wall of the gastrointestinal tract. Scand J Gastroenterol 19:315–320, 1984
Winne D, Gorig H: Appearance of [14C polyethylene glycol 4000 in intestinal venous blood: Influence of osmolarity and laxatives, effect on net water flux determination. Naunyn-Schmiedeberg's Arch Pharmacol 321:149–156, 1982
Hollander D, Dadufalza VD, Sletten EG: Does essential fatty acid absorption change with aging? J Lipid Res 25:129–134, 1984
Hollander D, Tarnawski H: Influence of aging on vitamin D absorption and unstirred water layer dimensions in the rat. J Lab Clin Med 103:462–469, 1984
Hollander D, Dadufalza VD: Aging associated pancreatic exocrine insufficiency in the unanesthetized rat. Gerontology 30:218–222, 1984
Hollander D, Tarnawski H: Aging associated increase in intestinal absorption of macromolecules. Gerontology 31:133–137, 1985
Editorial: Intestinal permeability. Lancet, 2:256–258, 1985
Barrows CH Jr, Falzone JA Jr, Shock NW: Age-related differences in the succinoxidase activity of homogenates and mitochondria from the livers and kidneys of rats. J Gerontol 15:130–133, 1960
Beauchene RE, Fanestil DD, Barrows CH Jr: The effects of age on active transport and sodium-potassium-activated ATPase activity in renal tissues of rats. J Gerontol 20:306–310, 1965
Couser WG, Stilmant MM: Immunopathology of the aging rat kidney. J Gerontol 31:13–22, 1976
Shimizu F, Abe F, Ito K, Kawamura S: Age associated presence of immunoglobulin and complement in renal glomeruli in mice. Contrib Nephrol 6:79–93, 1977
Lin CF, Hayton WL: Absorption of polyethylene glycol 400 administered orally to mature and senescent rats. Age 6:52–56, 1983
Chow SL, Hollander D: A dual, concentration-dependent absorption mechanism of linoleic acid by rat jejunumin vitro. J Lipid Res 20:349–356, 1979
Meshkinpour H, Smith MS, Hollander D: Influence of aging on the surface area of small intestine in the rat. Exp Gerontol 16:399–404, 1981
Penzes L, Skala I: Changes in the mucosal surface area of the small gut of rats of different ages. J Anat 124:217–222, 1977
Madra JL, Trier JS, Neutra MR: Structural changes in the plasma membrane accompanying differentiation of epithelial cells in human and monkey small intestine. Gastroenterology 78:963–975, 1980
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These studies were supported by grant AG2767 from the National Institutes on Aging and by the Goldsmith Family Foundations.
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Katz, D., Hollander, D., Said, H.M. et al. Aging-associated increase in intestinal permeability to polyethylene glycol 900. Digest Dis Sci 32, 285–288 (1987). https://doi.org/10.1007/BF01297055
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DOI: https://doi.org/10.1007/BF01297055