Summary
Lucifer Yellow (LY), a membrane-impermeant anion, was able to enterArabidopsis thaliana cells. LY was taken up by fluid-phase endocytosis and a plasmalemmal anionic carrier mechanism. Both mechanisms were shown to be concentration-dependent. At 0.1 mg/ml, LY was mainly taken up via fluid-phase endocytosis and concentrated in vesicular-like structures. At a ten-fold higher concentration (1 mg/ml), a plasmalemmal anionic carrier system allowed LY uptake and its accumulation in the central vacuole by a vacuolar anionic transporter. Chloroquine, cytochalasin B, monensin, and phorbol-12-myristate-13-acetate (PMA) hindered LY endocytosis. Brefeldin A did not modify LY uptake. The probenecidsensitive carrier uptake machinery showed sensitivity to chloroquine and PMA. Therefore the probenecid-sensitive transport mechanism seems to be complex and involve both acidification of a compartment and protein kinase C activity.
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Abbreviations
- CH:
-
carbohydrazide
- DMSO:
-
dimethylsulfoxide
- LY:
-
Lucifer Yellow
- MES:
-
2-[N-morpholino]-ethanesulfonic acid
- MS:
-
Murashige and Skoog's medium
- PMA:
-
phorbol-12-myristate-13-acetate
- NAA:
-
naphthalene acetic acid
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Roszak, R., Rambour, S. Uptake of Lucifer Yellow by plant cells in the presence of endocytotic inhibitors. Protoplasma 199, 198–207 (1997). https://doi.org/10.1007/BF01294506
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DOI: https://doi.org/10.1007/BF01294506