Summary
The green algaScenedesmus quadricauda (Turp.) Bréb. was cultivated in the presence or absence of orthophosphate and synchronized daughter or mother cells were cytochemically stained. Forin situ capturing of water soluble phosphates Ca2+ and Mg2+ ions were added to the ice-cold glutaraldehyde fixative to form a polymeric metal-phosphate complex which was equivalent to the energy-rich condensed polyphosphates in staining by alkaline lead acetate. The X-ray microanalysis of the extensive stained deposits proved the presence of phosphorus. In orthophosphate-supplied daughter cells cytoplasmic vacuoles contained round stained bodies; a layer of phosphate-containing paracrystals encompassing some starch grains and a fine stained layer delineating the chloroplast envelope were also observed. In the equivalent mother cells only the material inside theloculi of stacked thylakoids was stained. In orthophosphate starved daughter cells filamentous phosphate-containing paracrystals filled extensive cytoplasmic vacuoles. A stained layer covered the chloroplast envelope and continuous stained layers appeared inside theloculi of stacked thylakoids. Mother cells that develop from these daughter cells were filled with starch grains and showed only peripheral stained deposits. The results are compared with the biochemical evidence of phosphate turnover in algal cells.
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Abbreviations
- ADP:
-
adenosine diphosphate
- ATP:
-
adenosine triphosphate
- ATPase:
-
adenosine triphosphatase
- EDAX:
-
energy dispersive analysis of X-rays
- Pi:
-
orthophosphate
- PPi:
-
pyrophosphate
- PP:
-
polyphosphate
- PhAR:
-
photosynthetic active radiation
- TCA:
-
trichloroacetic acid
References
Atkinson, A. W., John, P. C. L., Cunny, B. E. S., 1974: The growth and division of the single mitochondria and other organelles during the cell cycle ofChlorella studied by quantitative stereology and three dimensional reconstruction. Protoplasma81, 77–100.
Béliveau, B., Bellamare, G., 1979a: Light-dependent phosphorylation of thylakoid membrane polypeptides. Biochem. biophys. Res. Commun.88, 797–803.
— —, 1979b: Thylakoid membrane protein phosphorylation in correlation with photosynthetic membrane action. Biochem. biophys. Res. Commun.91, 1377–1382.
Brierley, G. P., Slautterback, D. B., 1964: Studies on ion transport IV. An electron microscope study of the accumulation of Ca2+ and inorganic phosphate by heart mitochondria. Biochim. biophys. Acta82, 183–186.
Chen-She, S. H., Lewis, D. H., Walker, D. A., 1975: Stimulation of photosynthetic starch formation by sequestration of cytoplasmic orthophosphate. New Phytologist74, 381–390.
Colquhoun, W. R., Rieder, C. L., 1980: Contrast enhancement based on rapid dehydration in the presence of phosphate buffer. J. Ultrastruct. Res.73, 1–8.
Dawes, E. A., Senior, P. J., 1973: The role and regulation of energy reserve polymers in micro-organisms. In: Adv. Microbiol. Physiol.. (Rose, A. H., Tempest, D. W., eds.), pp. 136–266. London-New York: Academic Press.
Elgavish, A., Elgavish, G. A., 1980:31P-NMR differentiation between intracellular phosphate pools inCosmarium (Chlorophyta). J. Phycol.16, 368–374.
Feindt, F., Mendgen, K., Heitefuss, R., 1981: Feinstruktur unterschiedlicher Zellwandreaktionen im Blattparenchym anfälliger und resistenter Rüben (Bata vulgaris L.) nach Infektion durchCercospora beticola Sacc. Phytopath. Z.101, 248–264.
Firth, J. A., 1978: Cytochemical approaches to the localization of specific adenosine triphosphatases. Histochem. J.10, 253–269.
Haggard, S. S., Regan, P. A., 1977: Electron optical artifacts and chloroplast membranes. J. Electron Microsc.26, 161–163.
Harold, F. M., 1966: Inorganic polyphosphate in biology: Structure, metabolism, and function. Bacteriol. Rev.30, 772–794.
Heber, U., 1974: Metabolite exchange between chloroplasts and cytoplasm. Ann. Rev. Plant Physiol.25, 393–421.
Jensen, T. E., Sicko, L. M., 1974: Phosphate metabolism in bluegreen algae. I. Fine structure of the polyphosphate overplus phenomenon inPlectonema boryanum. Can. J. Microbiol.20, 1235–1239.
Kulaev, I. S., 1975: Biochemistry of inorganic polyphosphates. Rev. Physiol. Biochem. Pharmacol.73, 131–158.
Langenberg, W. G., 1979: Chilling of tissue before glutaraldehyde fixation preserves fragile inclusions of several plant viruses. J. Ultrastruct. Res.66, 120–131.
Miller, K. R., 1979: The photosynthetic membrane. Sci. Amer.241, 102–113.
Nicolson, G. L., 1971: Structure of the photosynthetic apparatus in protein-embedded chloroplasts. J. Cell Biol.50, 258–263.
Nilshammar-Holmvall, M., 1976: The cyclic variation in the thylakoid aggregation in normal and calcium deficient cells ofScenedesmus. Protoplasma87, 262–271.
Nilshammar, M., Wales, B., 1974: Electron microscope studies on cell differentiation in synchronized cultures of the green algaScenedesmus. Protoplasma79, 317–332.
Poux, N., 1965: Mise en évidence des phosphates en microscopie électronique dans les cellules embryonnaires de quelques dicotylédones. C. R. Acad. Sci. Paris261, 1064–1066.
Reynolds, F. S., 1963: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. biophys. biochem. Cytol.17, 208–212.
Sigler, K., Kotyk, A., 1976: Some properties of the adenosine triphosphate systems of two yeast species,Saccharomyces cerevisiae andRhodotorula glutinis. Mol. Cell Biochem.12, 73–79.
Stewart, W. D. P., Pemble, M., Al-Ugaily, L., 1978: Nitrogen and phosphorus storage and utilization in blue-green algae. Mitt. Internat. Verein. Limnol.21, 224–247.
Sundberg, L, Nilshammar-Holmvall, M., 1975: The diurnal variation in phosphate uptake and ATP level in relation to deposition of starch, lipid and polyphosphate in synchronized cells ofScenedesmus. Zeitschr. Pflanzenphysiol.76, 270–279.
Tillberg, J. E., Barnard, T., Rowley, J., 1979: X-ray microanalysis of phosphorus inScenedesmus obtusiusculus. Physiol. Plant.47, 34–38.
Tokuyashu, K. T., 1974: A technique for ultracryotomy of cell suspensions and tissues. J. Cell Biol.57, 551–565.
Vandegrift, V., Evans, R. R., 1981: Polyphosphate binding interactions with bovine serum albumin in proteinpolyphosphate precipitates. J. Agr. Food Chem.29, 536–539.
Van Steveninck, R. F. M., 1979: Verification of cytochemical tests for ATPase activity in plant cells using X-ray microanalysis. Protoplasma99, 211–220.
Vaughn, K. C., Duke, S. O., 1981: Cytochemical localization of photosystem II donor sites. Histochemistry73, 363–370.
Voříšek, J., Pokorný, V., 1975: Cytochemical detection of polysaccharides on the surface of the cell membrane complex in fungi. Arch. Microbiol.102, 293–298.
-Schwencke, J., 1984: Localization of (poly)phosphates in yeast cells by the Gomori staining. Interference of (poly)phosphatases. J. Histochem.16 (in press).
—,Knotková, A., Kotyk, A., 1982: Fine cytochemical localization of polyphosphates in the yeastSacharomyces cerevisiae. Zbl. Mikrobiol.137, 421–432.
Walker, D. A., 1976: Plastids and intracellular transport. In: Encyclopedia of Plant Physiology, New series, Volume 3: Transport in Plants III, Intracellular interactions and transport processes. (Stocking, C. R., Heber, U., eds.), pp. 85–136. Berlin-Heidelberg-New York: Springer.
Widra, A., 1959: Metachromatic granules of microorganisms. J. Bacteriol.78, 664–670.
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Voříšek, J., Zachleder, V. Redistribution of phosphate deposits in the algaScenedesmus quadricauda deprived of exogenous phosphate—an ultra-cytochemical study. Protoplasma 119, 168–177 (1984). https://doi.org/10.1007/BF01288871
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DOI: https://doi.org/10.1007/BF01288871