Summary
A morphological analysis of the initiation and development of root nodules ofElaeagnus angustifolia andMyrica cerifera inoculated with pure-culturedFrankia strains DDB 011610 or DDB 020110 was undertaken. From ultrastructural observations it was determined that both of theseFrankia strains can infectElaeagnus by an intercellular penetration mechanism andMyrica by the root hair infection mechanism. This indicates that both of these strains have the ability to infect host plant roots by either of two mechanisms. The reverse, thatElaeagnus orMyrica could be infected by both mechanisms, was not observed. The infection and nodule development processes of these two plants in combination with these strains were similar to observations made in previous studies (Miller andBaker 1985,Torrey andCallaham 1979). However, one exception was identified in the development of the prenodule ofMyrica when infected with strain 011610, in that endophytic hyphae developed vesicles within the cells of the prenodule. This event has not been described before for any of the actinorhizal genera and may be an indication of less than optimal compatibility between the host plant and the symbiont.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Baker, D., O'Keefe, D., 1984: A modified sucrose fractionation procedure for the isolation ofFrankiae from actinorhizal root nodules and soil samples. Plant Soil78, 23–28.
Benson, D. R., Eveleigh, D. E., 1979: Ultrastructure of the nitrogen-fixing symbiont ofMyrica pensylvanica L. (Bayberry) root nodules. Bot. Gaz.140(S), 15–21.
Berry, A. M., 1984: The actinorhizal infection process: review of recent research. In: Current Perspectives in Microbial Ecology (Klug, M., Reddy, C., eds.), pp. 222–229. Washington: Amer. Soc. Microbiol.
Bond, G., 1951: The fixation of nitrogen associated with the root nodules ofMyrica gale L., with special reference to its pH relation and ecological significance. Ann. Bot.15, 447–459.
Callaham, D., Newcomb, W., Torrey, J. G., Peterson, R. L., 1979: Root hair infection in actinomycete-induced root nodule initiation inCasuarina, Myrica, andComptonia. Bot. Gaz.140(S), 1–9.
—,Torrey, J. G., 1977: Prenodule formation and primary nodule development in roots ofComptonia (Myricaceae). Can. J. Bot.55, 2306–2318.
Gauthier, D., Frioni, L., Diem, H. G., Dommergues, Y., 1984: TheColletia spinosissima-Frankia symbiosis. Acta Oecologica/Oecologia Plantarum5, 231–239.
Lalonde, M., 1979: Immunological and ultrastructural demonstration of nodulation of the EuropeanAlnus glutinosa (L.) Gaertn. host plant by an actinomycetal isolate from the North AmericaComptonia peregrina (L.) Coult. root nodule. Bot. Gaz.140(S), 35–43.
—,Knowles, R., 1975: Ultrastructure, composition, and biogenesis of the encapsulation material surrounding the endophyte inAlnus crispa var.mollis root nodules. Can. J. Bot.53, 1951–1971.
Miller, I. M., Baker, D. D., 1985: The initiation, development and structure of root nodules inElaeagnus angustifolia L. (Elaeagnaceae). Protoplasma128, 107–119.
Rodriques-Barrueco, C., Miguel, C., 1979: Host plant-endophyte specificity in actinomycete-nodulated plants. In: Symbiotic Nitrogen Fixation in the Management of Temperate Forests (Gordon, J., Wheeler, C., Perry, D., eds.), pp. 143–159. Corvallis: Oregon State University Forest Research Laboratory.
— —,Cañizo, A., 1979: Host plant endophyte specificity in actinorhizal plants. In: Current Perspectives in Nitrogen Fixation (Gibson, A., Newton, W., eds.), pp. 476. Canberra: Aust. Acad. Sci.
Saint-Laurent, L.,Lalonde, M., 1984: Isolation and characterization ofFrankia strains fromMyrica gale L. Paper presented at Montmorency Forest, Laval University, August 1984.
Torrey, J. G., Callaham, D., 1979: Early nodule development inMyrica gale. Bot. Gaz.140(S), 10–14.
Author information
Authors and Affiliations
Additional information
Contribution no. 876 of the Battelle-Kettering Laboratory.
Rights and permissions
About this article
Cite this article
Miller, I.M., Baker, D.D. Nodulation of actinorhizal plants byFrankia strains capable of both root hair infection and intercellular penetration. Protoplasma 131, 82–91 (1986). https://doi.org/10.1007/BF01281689
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01281689