Summary
The localization of β-glucosidases in roots of garbanzo beans [Cicer arietinum (L.)] has been investigated by a one-step fluorescence optical procedure using 4-methylumbelliferyl-β-D-glucoside. It is shown that the well known azo-coupling test for the localization of glucosidases cannot be applied in polyphenol containing plants. The results obtained with the new method are comparable with those described in other investigations and further show that α-glucosidases are absent from the root tissues investigated. The aryl-β-glucosidases were mainly detected in the outer region of the cortex and quantitatively increase towards the root tip.
Zusammenfassung
Die Lokalisierung von β-Glucosidasen in Wurzeln der Kichererbse [Cicer arietinum (L.)] wurde in einem einstufigen fluoreszenz-optischen Verfahren mit 4-Methyl-umbelliferyl-βD-glucosid untersucht. Am Beispiel der Kichererbse wird gezeigt, daß in polyphenolhaltigen Pflanzen herkömmliche Azokupplungsverfahren zur Glucosidaselokalisierung nicht anwendbar sind. Die mit der neuen Methode erhaltenen Ergebnisse decken sich im wesentlichen mit denen aus vergleichbaren Untersuchungen und zeigen, daß α-Glucosidasen nicht vorhanden sind. Aryl-β-Glucosidasen wurden vorwiegend im äußeren Cortexbereich gefunden und nehmen mengenmäßig zur Wurzelspitze hin zu.
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Literatur
Ashford, A. E., 1970: Histochemical Localization of β-glucosidases in roots ofZea mays. I. A Simultaneous azo-dye coupling technique for the localization of β-glucosidase and β-galactosidase. Protoplasma71, 281–293.
—, andM. E. McCully, 1970: II. Changes in localization and activity of β-glucosidase in the main root apex. Protoplasma71, 389–402.
Barz, W., undH. Grisebach, 1967: Untersuchungen über den Einbau methylierter Vorstufen in die Isoflavone der Kichererbse. Z. Naturforsch.226, 627.
Beneš, K., Y. D. Georgieva, andD. Polakova, 1973: The presence and distribution of α- and β-glucosidases in root tip. Biol. Plant.15, 88–94.
Cohen, R. B., S. H. Rutenberg, K. C. Tsou, W. S. Woodbury, andA. M. Seligmann, 1952: The colorimetric estimation of β-D-glucosidase. J. biol. Chem.195, 607–614.
Hoesel, W., und W.Barz, 1975: β-Glucosidase fromCicer arietinum purification and properties of isoflavone-7-o-glucoside specific β-glucosidases. Europ. J. Biochem., im Druck.
Mace, M. E., 1973: Histochemistry of β-Glucosidase in isolines ofZea mays susceptible or resistant to northern corn leaf blight. Phytopathology63 (2), 243.
Onofeghara, F. A., 1973: Histochemical localization of enzymes inTapinanthus bangwensis and its hosts: Alkaline phosphatase and β-glucosidase. Bot. Gaz.134 (1), 36–46.
Page-Thomas, D. P., 1970: Automated fluorometric enzyme assay using a Unicam AC 60 Automatic Analytical system. Spectrovision24, 3–6.
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Gierse, H.D., Barz, W. Ein fluoreszenzmikroskopischer Nachweis von β-Glucosidase in Wurzeln vonCicer arietinum (L.). Protoplasma 88, 1–6 (1976). https://doi.org/10.1007/BF01280355
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DOI: https://doi.org/10.1007/BF01280355