Summary
Ascorbate is stabilized in the presence of HL-60 cells. Our results showed that cAMP derivatives and agents that increase cAMP stimulate the ability of HL-60 cells to stabilize ascorbate. On the other hand, tunicamycin, a glycosilation-interfering agent, inhibited this ability. The ascorbate stabilization in the presence of HL-60 cells has been questioned as a simple chemical effect. Further properties and controls about the enzymatic nature of this stabilization are described and discussed. This data, together with hormonal regulation, support the hypothesis that an enzymatic redox system located at the plasma membrane is responsible of the extracellular ascorbate stabilization by HL-60 cells.
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Abbreviations
- AFR:
-
ascorbate free radicals
- FCS:
-
fetal calf serum
- Sp-cAMPS:
-
Sp-cyclic adenosine monophosphothionate
- Rp-cAMPS:
-
Rp-cyclic adenosine monophosphothionate
References
Alcaín FJ, Burón MI, Villalba JM, Navas P (1991) Ascorbate is regenerated by HL-60 cells through the transplasmalemma redox system. Biochim Biophys Acta 1073: 305–380
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254
Brown WJ, Constantinescu E, Farquhar MG (1984) Redistribution of mannose-6-phosphate receptors induced by tunicamycin and chloroquine. J Cell Biol 99: 320–326
Burón MI, Navas P (1990) Roles of pyridine nucleotides in the control of cell growth. In: Moiré DJ, Crane FL, Löw H (eds) Oxido-reduction at the plasma membrane: relation to growth and transport, vol 1, animals. CRC Press, Boca Raton, pp 226–236
Coassin M, Tomasi A, Vannini V, Ursini F (1991) Enzymatic recycling of oxidized ascorbate in pig heart: one-electron vs two-electron pathway. Arch Biochem Biophys 290: 458–462
Crane FL, Löw H, Sun IL, Isaksson M (1990) Transmembrane electron transport and growth of transformed cells. In: Morré DJ, Crane FL, Löw H (eds) Oxidoreduction at the plasma membrane: relation to growth and transport, vol 1, animals. CRC Press, Boca Raton, pp 141–170
Crowe RA, Taparowsky EJ, Crane FL (1993)Ha-ras stimulates the transplasma membrane oxidoreductase activity of C3H 10T1/2 cells. Biochem Biophys Res Commun 196: 844–850
Lesuisse E, Horion B, Labbe P, Hilger F (1991) The plasma membrane ferrireductase activity ofSaccharomyces cerevisiae is partially controlled by cyclic AMP. Biochem J 280: 545–548
Medina MA, del Castillo-Olivares A, Schweigerer L (1992) Plasma membrane redox activity correlates with N-myc expression in neuroblastoma cells. FEBS Lett 311: 99–101
Minetti M, Forte T, Soriani M, Quaresima V, Menditto A, Ferrari M (1992) Iron-induced ascorbate oxidation in plasma as monitored by ascorbate free-radical formation — no spin-trapping evidence for the hydroxyl radical in iron-overloaded plasma. Biochem J 282: 459–465
Navas P, Alcaín FJ, Burón MI, Rodríguez-Aguilera JC, Villalba JM, Morré DM, Morré DJ (1992) Growth factor-stimulated trans plasma membrane electron transport in HL-60 cells. FEBS Lett 299: 223–226
—, Estévez A, Burón MI, Villalba JM, Crane FL (1988) Cell surface glycoconjugates control the activity of the NADH-ascorbate free radical reductase of rat liver plasma membrane. Biochem Biophys Res Commun 154: 1029–1033
Rose RC, Bode AM (1993) Biology of free radical scavengers: an evaluation of ascorbate. FASEB J 7: 1135–1142
Schweinzer E, Goldenberg H (1992) Ascorbate-mediated transmembrane electron transport and ascorbate uptake in leukemic cell lines are two different processes. Eur J Biochem 206: 807–812
—, Waeg G, Esterbauer H, Goldenberg H (1993) No enzymatic activities are necessary for the stabilization of ascorbic acid by K562 cells. FEBS Lett 334: 106–108
Sthal RL, Lieber LF, Silber R (1985) A reappraisal of leukocyte dehydroascorbate reductase. Biochim Biophys Acta 839:119–121
Villalba JM, Canalejo A, Burón MI, Córdoba F, Navas P (1993) Thiol groups are involved in NADH-ascorbate free radical reductase activity of rat liver plasma membrane. Biochem Biophys Res Commun 192: 707–713
von Figura K, Rey M, Prinz R, Voss B, Ullrich K (1979) Effect of tunicamycin on transport of lysosomal enzymes in cultured skin fibroblasts. Eur J Biochem 101: 103–109
Wunderling M, Paul HH, Lohmann W (1986) Evaluation of a direct spectrophotometric method for the rapid determination of ascorbate and dehydroascorbate in blood using ascorbate oxidase. Biol Chem Hoppe Seyler 367: 1047–1054
Yoshikawa K, Takeda J, Nemoto O, Halprin KM, Adachi K (1981) Activation of cAMP-dependent protein kinase in epidermis by the compounds which increase epidermal cAMP. J Invest Dermatol 77: 397–401
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Rodríguez-Aguilera, J.C., Navarro, F., Arroyo, A. et al. Vitamin C stabilization as a consequence of the plasma membrane redox system. Protoplasma 184, 229–232 (1995). https://doi.org/10.1007/BF01276925
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DOI: https://doi.org/10.1007/BF01276925