Summary
N-acetylchitooligosaccharides, fragments of the backbone of fungal cell wall, trigger rapid membrane responses such as transient depolarization, and elicit defense reactions including phytoalexin production in suspension-cultured rice cells. The generation of reactive oxygen species triggered by the oligosaccharide signal was analyzed with EPR spectroscopy using a spin trapping system, 4-pyridyl 1-oxideN-tert-butyl nitrone (4-POBN) and ethanol. OH generation was detected as the α-hydroxyethyl adduct of 4-POBN after elicitation. Superoxide dismutase, catalase or diethylenetriamine pentaacetic acid, a metal chelator, inhibited\({}^ \cdot O\) generation, proposing the following reaction sequence: generation of\({}^ \cdot O_{2^ - } \) in response to the oligosaccharide elicitor, followed by dismutation to H2O2, then generation of\({}^ \cdot OH\) by the reaction of H2O2 with Fe2+ that is generated by the reduction of Fe3+ by\({}^ \cdot O_{2^ - } \). Generation of the same reactive oxygen species was also triggered by calyculin A, a protein phosphatase inhibitor, alone, suggesting the involvement of protein phosphorylation in its regulation during the oligosaccharide signal transduction.
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Abbreviations
- DMPO:
-
5,5-dimethyl-1-pyrroline N-oxide
- DTPA:
-
diethylenetriamine pentaacetic acid
- 4-POBN:
-
4-pyridyl 1-oxideN-tert-butylnitrone
- SOD:
-
Superoxide dismutase
- 4-hydroxy-TEMPO:
-
2,2,6,6-tetramethyl-4-hydroxypiperidine-1-oxyl
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Kuchitsu, K., Kosaka, H., Shiga, T. et al. EPR evidence for generation of hydroxyl radical triggered byN-acetylchitooligosaccharide elicitor and a protein phosphatase inhibitor in suspension-cultured rice cells. Protoplasma 188, 138–142 (1995). https://doi.org/10.1007/BF01276805
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DOI: https://doi.org/10.1007/BF01276805