Abstract
Excised seedling leaf segments of winged bean [Psophocarpus tetragonolobus (L.) DC.] underwent direct somatic embryogenesis under appropriate incubation conditions. Initiation and development of the somatic embryos occurred using a two-step culture method. The culture procedure involved incubation for 28 days on MS basal medium supplemented with 0.1–0.5 mg/l NAA and 1.0–2.0 mg/l BA (induction medium) before transfer to MS medium supplemented with 0.1 mg/l IAA and 2.0 mg/l BA (embryo development medium). The initial exposure to low levels of NAA coincident with high levels of BA in the induction medium was essential for embryogenic induction. Maximum embryogenesis (43.3%) was obtained with 0.2 mg/l NAA and 2.0 mg/l BA, and at least 14 days on induction medium were required prior to transfer to the embryo development medium. The conversion frequency of cotyledonary embryos was 53.3% upon culture on MS medium containing 0.1 mg/l ABA for 7 days followed by transfer to MS medium supplemented with 0.1 mg/l IBA and 0.2 mg/l BA. Following conversion, the regenerated plantlets were transferred to soil and showed normal morphological characteristics.
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Abbreviations
- MS :
-
Murashige and Skoog (1962) medium
- 2,4-D :
-
2,4-dichlorophenoxyacetic acid
- NAA :
-
1-naphthaleneacetic acid
- IAA :
-
indole-3-acetic acid
- IBA :
-
indole-3-butyric acid
- BA :
-
6-benzylaminopurine
- ABA :
-
abscisic acid
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Dutta Gupta, S., Ahmed, R. & De, D.N. Direct somatic embryogenesis and plantlet regeneration from seedling leaves of winged bean,Psophocarpus tetragonolobus (L.) DC. Plant Cell Reports 16, 628–631 (1997). https://doi.org/10.1007/BF01275504
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DOI: https://doi.org/10.1007/BF01275504