Abstract
Invitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott.) were successfully cryopreserved by vitrification. Excised shoot tips precultured on solidified MS supplemented with 0.3M sucrose and maintained under a 16 h phtoperiod at 25°C for 16 h were loaded with a mixture of 2M glycerol plus 0.4M sucrose for 20 min at 25°C. The shoot tips were then sufficiently dehydrated with a highly concentrated vitrification solution (PVS2) for 20 min at 25°C prior to immersion into liquid nitrogen. Successfully vitrified and warmed shoot tips resumed growth within 7 days and developed shoots directly without intermediate callus formation. The average rate of shoot recovery amounted to around 80%, and the vitrification protocol appeared to be very promising for the cryopreservation of taro germplasm.
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Abbreviations
- DMSO :
-
Dimethylsulfoxide
- EG :
-
ethylene glycol
- LN :
-
liquid nitrogen
- MS :
-
Murashige & Skoog medium (1962)
- TDZ :
-
thidiazuron
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Communicated by A. Kornamine
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Takagi, H., Tien Thinh, N., Islam, O.M. et al. Cryopreservation of invitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure. Plant Cell Reports 16, 594–599 (1997). https://doi.org/10.1007/BF01275498
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DOI: https://doi.org/10.1007/BF01275498