Summary
Treatment with lysine prior to fixation of tobacco BY-2 cells with formaldehyde improved the preservation of actin filaments in the cells and enabled us to observe both networks of actin filaments and microtubules in the same cells. By using this method, we observed that (1) actin filaments were present in the preprophase band; (2) the actin filaments in the preprophase band and phragmoplast were runnig in the same direction as the microtubules in their respective structures; (3) a cortical network of actin filaments was present throughout all stages of cell cycle.
The present method did not preserve the cortical actin filaments in interphase cells. The procedure for staining microtubules destroyed them.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- EGTA:
-
Ethyleneglycol-bis(β-aminoethyl ether)N,N,N′,N′-tetraacetic acid
- PIPES:
-
Piperazine-N,N′-bis(2-ethanesulfonic acid)
- PMSF:
-
Phenylmethylsulfonyl fluoride
- TLCK:
-
Na-p-tosyl-L-lysine chloromethyl ketone
References
Boyles J, Anderson L, Hutcherson P (1985 a) A new fixative for the preservation of actin filaments: Fixation of pure actin filament pellets. J Histochem Cytochem 33: 1116–1128
Boyles J, Fox JEB, Phillips DR, Stenberg PE (1985b) Organization of the cytoskeleton in resting, discoid platelets: Preservation of actin filaments by modified fixation that prevents osmium damage. J Cell Biol 101: 1463–1472
Gunning BSE, Wick SM (1985) Preprophase bands, phragmoplasts, and spatial control of cytokinesis. J Cell Sci (Suppl) 2: 157–179
Clayton L, Lloyd CW (1985) Actin organization during the cell cycle in meristematic plant cells. Exp Cell Res 156: 231–238
Lehrer SS (1981) Damage to actin filaments by glutaraldehyde: Protection by tropomyosin. J Cell Biol 90: 459–466
Nagata T, Okada K, Takebe I, Mitsui C (1981) Delivery of tobacco mosaic virus RNA into plant protoplasts mediated by reverse-phase evaporation vesicles (liposomes). Mol Gen Genet 184: 161–165
Parthasarathy MV (1985) F-actin architecture in coleoptile epidermal cells. Eur J Cell Biol 39: 1–12
—,Perdue TD, Witztum A, Alvernaz J (1985) Actin network as a normal component of the cytoskeleton in many vascular plant cells. Am J Bot 72: 1318–1323
Pesacreta TC, Carley WW, Webb WW, Parthasarathy MV (1982) F-actin in conifer roots. Proc Natl Acad Sci USA 79: 2898–2901
Pierson ES, Derkson J, Traas JA (1986) Organization of microfilaments and microtubules in pollen tubes grownin vitro orin vivo in various angiosperms. Eur J Cell Biol 41: 14–18
Rodrigues J, Deinhardt F (1960) Preparation of a semipermanent mounting medium for fluorescent antibody studies. Virology 12: 316–317
Schmit AC, Lambert AM (1985) F-actin dynamics is associated to microtubule function during cytokinesis in higher plants. A revised concept. In:de Brabander, De May J (eds) Microtubules and microtubule inhibitors 1985. Elsevier Science Publishers, Amsterdam, pp 243–252
—,Ventard M, Lambert AM (1985) Microtubule and F-actin rearrangement during the initiation of mitosis in acentriolar higher plant cells. In:Ishikawa H, Hatano S, Sato H (eds) Cell motility: Mechanism and regulation. Univ Tokyo Press, Tokyo, pp 415–433
Tiwari SC, Wick SM, Williamson RE, Gunning BES (1984) Cytoskeleton and integration of cellular function in cells of higher plants. J Cell Biol 99: 63s-69s
Wick SM, Seagull RW, Osborn M, Weber K, Gunning BES (1981) Immunofluorescence microscopy of organized microtubule array in structually stabilized meristematic plant cells. J Cell Biol 89: 685–690
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kakimoto, T., Shibaoka, H. Actin filaments and microtubules in the preprophase band and phragmoplast of tobacco cells. Protoplasma 140, 151–156 (1987). https://doi.org/10.1007/BF01273724
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01273724