Summary
The preparation and maintenance of a culture of bovine turbinate cells is described. The cell line is free of bovine viral diarrhea, parainfluenza-3, and infectious bovine rhinotracheitis viruses; and although the modal chromosome number changed significantly between the 9th passage and the 81st passage, no definite morphological changes were observed. Low- and high-passage bovine turbinate cells were equally susceptible to bovine viral diarrhea virus.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Carbrey, E. A., L. N. Brown, T. L. Chow, R. F. Kahrs, D. G. McKercher, L. K. Smithies, andT. W. Tamoglia: Recommended standard laboratory techniques for diagnosis infectious bovine rhinotracheitis, bovine virus diarrhea, and shipping fever (parainfluenza-3). Proc. U.S. Anim. Hlth Ass.75, 629–648 (1971).
Edward, A. G., J. R. Calhoon, andG. D. Mills: Production of colostrum-deprived specific pathogen free calves. Lab. anim. Care17, 102–107 (1967).
Gillespie, J. A., J. A. Baker, andK. McEntee: A cytopathogenic strain of virus diarrhea virus. Cornell Vet.50, 73–79 (1960).
Gutekunst, D. E., andW. A. Malmquist: Separation of a soluble antigen and infectious particles of bovine viral diarrhea viruses and their relationship to hog cholera. Canad. J. comp. Med.27, 121–123 (1963).
Pirtle, E. C.: Chromosomal variations in a pig kidney cell line persistently infected with hog cholera virus. Amer. J. vet. Res.27, 737–745 (1966).
Reed, L. J., andH. Muench: A simple method of estimating 50% end-points. Amer. J. Hyg.27, 493–497 (1938).
Singh, K. V.: A study of bovine virus diarrhea mucosal disease virus by plaque technique. Canad. J. comp. Med.33, 207–213 (1969).
Underdahl, N. R., O. D. Grace, andA. B. Horlein: Cultivation in tissue culture of cytopathogenic agents from bovine mucosal disease. Proc. Soc. exp. Biol. (N.Y.)94, 795–797 (1957).
Author information
Authors and Affiliations
Additional information
Mention of commercial products does not constitute endorsement.
Rights and permissions
About this article
Cite this article
McClurkin, A.W., Pirtle, E.C., Coria, M.F. et al. Comparison of low- and high-passage bovine turbinate cells for assay of bovine viral diarrhea virus. Archiv f Virusforschung 45, 285–289 (1974). https://doi.org/10.1007/BF01249692
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01249692