Summary
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1.
Vegetative tissues ofFucus have been fixed and embedded using new methods which greatly improve the resolution of histological detail.
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2.
The mature thallus consists of an outer layer of columnar epidermal cells, several underlying layers of parenchyma, and a medullary region of branched primary filaments and elongated secondary fibres embedded in a metachromatic matrix. The holdfast is made up of an outer calluslike cortex and a medulla of thick-walled fibres also embedded in metachromatic extracellular material.
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3.
The growing point of the thallus lies at the base of an apical groove. In the plants examined which were rapidly growing, there is a group of 4 to 8 apical initials.
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4.
The results of toluidine blue staining and the PAS reaction indicate that the alginic acid and fucoidin of the extracellular matrix are synthesized within the cells and secreted through the plasma membrane.
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The polarized epidermal cells are apparently specialized for the secretion of polysaccharides to the outside of the plant.
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6.
The results of toluidine blue staining suggest that there are possibly 3 different types of polyphenol-containing vesicles present. Two of these types are found in the cells of the thallus and the third is confined to cells of the holdfast.
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References
Ando, Y., 1958 a: Studies on physodes. 1. Bull. Japan. Soc. Phycol.6, 28–34.
—, 1958 b: Studies on physodes. 2. Bull. Japan. Soc. Phycol.6, 45–50.
Ashley, C. A., and N. Feder, 1966: Glycol methacrylate in histopathology. A study of central necrosis of the liver using a water-miscible plastic as embedding medium. Archives of Pathology81, 391–397.
Barka, T., and P. J. Anderson, 1963: Histochemistry, Theory, Practice, and Bibleography. Harper, Roe and Co., New York.
Berendes, H. D., 1965: Salivary gland function and chromosomal puffing patterns inDrosophila hydei. Chromosoma (Berl.)17, 35–77.
Black, W. A. P., et al., 1953: Constituents of the marine algae. Ann. Repts. on Progr. Chem. (Chem. Soc. London)50, 322–335.
Buer, F., 1964: Licht- und elektronenmikroskopische Untersuchungen an Zellwänden und Gallerten einiger Zygnemataceen. Flora154, 349–375.
Chadefaud, M., 1932: Sur les physodes des Phéophycées. C. r. Acad. Sci.194, 1675–1677.
Conchie, J., and E. G. V. Percival, 1950: Fucoidin. Part II. The hydrolysis of a methylated fucoidin prepared fromFucus vesiculosus. J. Chem. Soc. London, Part 1, 827–832.
Craigie, J. S., and J. McLachlan, 1964 a: Excretion of colored ultravioletabsorbing substances by marine algae. Can. J. Bot.42, 23–33.
—, 1964 b: Algal inhibition by yellow ultraviolet-absorbing substances fromFucus vesiculosus. Can. J. Bot.42, 287–292.
Crato, E., 1893: Morphologische und mikrochemische Untersuchungen über die Physoden. Bot. Z.51, 157–195.
Danielli, J. F., 1949: A critical study of techniques for the cytochemical demonstration of aldehydes. Quat. J. microsc. Sci.90, 67–74
Drawert, H., and M. Mix, 1961: Licht- und elektronenmikroskopische Untersuchungen an Desmidiaceen. II. Mitt. Hüllgallerte und Schleimbildung beiMicrasterias, Pleurotaenium undHyalotheca. Planta56, 237–261.
Elliot, E., 1951: Formation of new cell walls in cell division. Nature168, 1089.
Farmer, J. B., and J. L. Williams, 1898: Contributions to our knowledge of the Fucaceae: their life-history and cytology. Phil. Trans. Roy. Soc. London B190, 623–645.
Feder, N., 1960: Some modifications in conventional techniques of tissue preparation. J. Histochem. Cytochem.8, 309–310.
Fritsch, F. E., 1945: The Structure and Reproduction of the Algae II. University Press, Cambridge.
Godman, G. C., and N. Lane, 1964: On the site of sulphation in the chondrocyte. J. Cell Biol.21, 353–366.
Hick, T., 1885: Protoplasmic continuity in the Fucaceae. J. Bot.23, 97–102.
Hotchkiss, R. D., 1948: A microchemical reaction resulting in the staining of polysaccharide structures in fixed tissue preparations. Arch. Biochem.16, 131–141.
Hršel, I., J. Juráková, and K. Beneš, 1960: Cytochemical and electronmicroscope investigations of the Golgi apparatus ofFagopyrum esculentum Moench. Biol. Plant. (Praha)2, 252–268.
Jensen, W. A., 1962: Botanical Histochemistry, W. H. Freeman and Co., San Francisco.
Keck, K., and H. Stich, 1957: The widespread occurrence of polyphosphate in lower plants. Ann. Bot. N. S.21, 611–619.
Kreger, D. R., 1962: Cell walls. In: Physiology and Biochemistry of Algae. Edited by R. A. Lewin, Academic Press, New York.
Kuhl, A., 1962: Inorganic phosphorus uptake and metabolism. In: Physiology and Biochemistry of Algae. Edited by R. A. Lewin. Academic Press, New York.
McCully, M. E., 1965: A note on the structure of the cell walls of the brown algaeFucus. Can. J. Bot.43, 1001–1004.
—, 1966: Correlated light and electron microscope studies of the cell walls ofFucus. In: Proceedings of the Fifth International Seaweed Symposium. Pergamon Press, Oxford (in press).
MacPherson, M. G., and E. G. Young, 1952: Seasonal variation in the chemical composition of the Fucaceae in the Maritime Provinces. Can. J. Bot.,30, 67–77.
Meeuse, B. J. D., 1962: Storage products. In: Physiology annd Biochemistry of Algae. Edited by R. A. Lewin, Academic Press, New York.
Mollenhauer, H. H., W. G. Whaley, and J. H. Leech, 1961: A function of the Golgi apparatus in outer rootcap cells. J. Ultrastruct. Res.5, 193–200.
Moore, E., 1965: Fungal gel tissue ontogenesis. Amer. J. Bot.52, 389–395.
Moss, B. L., 1948: Studies in the genusFucus. I. On the structure and chemical composition ofFucus vesiculosus from three Scottish localities. Ann. Bot. N.S.12, 267–279.
—, 1950: Studies in thegenus Fucus. III. Structure and development of the attaching discs ofFucus vesiculosus. Ann. Bot. N.S.14, 411–419.
Northcote, D. H., and J. D. Pickett-Heaps, 1966: A function of the Golgi apparatus in polysaccharide synthesis and transport in the root-cap cells of wheat. Biochem. J.98, 159–167.
O'Brien, T. P., N. Feder, and M. E. McCully, 1964: Polychromatic staining of plant cell walls by toluidine blue O. Protoplasma59, 368–373.
O'Colla, P. S., 1962: Mucilages. In: Physiology and Biochemistry of Algae. Edited by R. A. Lewin, Academic Press, New York.
Oltmanns, F., 1889: Beiträge zur Kenntnis der Fucaceen. Bib. Bot.3, 1–94.
Percival, E., 1964: Algal polysaccharides and their biological relationships. In: Proceedings of the Fourth International Seaweed Symposium. Edited by Ad. Davey de Virville and J. Feldmann. Pergamon Press, Oxford.
Provasoli, L., 1965: Nutritional aspects of seaweed growth. Abs. Proc. Can. Soc. Plant Physiol.6, 26–27.
Rappay, Gy., and P. Van Duijn, 1965: Chlorous acid as an agent for blocking tissue aldehydes. Stain Tech.40, 275–277.
Reinke, J., 1876: Beiträge zur Kenntnis der Tange. Jb. wiss. Bot.10, 317–382.
Robinow, C. F., and J. Marak, 1966: A fibre apparatus in the nucleus of the yeast cell. In press, J. Cell Biol.
Rosen, W. G., et al., 1964: Fine structure and cytochemistry ofLilium pollen tubes. Amer. J. Bot.51, 61–71.
Rostafiński, J., 1876: Beiträge zur Kenntnis der Tange. Arthur Felix, Leipzig.
Roy, K., 1938: Recherches sur la structure du noyau quiescent et sur les mitosis somatiques de quelques Fucacées. Rev. Algol.11, 101–188.
Schnepf, E., 1963: Zur Cytologie und Physiologie pflanzlicher Drüsen. I. Über den Fangschleim der Insectivoren. Flora153, 1–22.
Sievers, A., 1963: Beteiligung des Golgi-Apparates bei der Bildung der Zellwand von Wurzelhaaren. Protoplasma56, 188–192.
Strasburger, E., 1887: Das Botanische Practicum. Gustav Fischer, Jena.
Wetmore, R. H., 1932: The use of celloidin in botanical technic. Stain Tech.7, 37–62.
Woodworth, W. M., 1888: The apical cell ofFucus. Ann. Bot.1, 203–211.
Yamanouchi, S., 1909: Mitosis inFucus. Bot. Gaz.47, 173–197.
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McCully, M.E. Histological studies on the genusFucus . Protoplasma 62, 287–305 (1966). https://doi.org/10.1007/BF01248267
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DOI: https://doi.org/10.1007/BF01248267