Summary
The viruses causing feline panleucopaenia and mink enteritis are shown to have the morphology of parvoviruses, with a mean diameter between 21 and 24 nm. Buoyant density studies show that purified virus of both strains consists of particles which band as “complete” virus at a main density of 1.41 g/ml, with a minor population at a density of 1.44 g/ml. Other particle species with a lower content of DNA, or no detectable DNA, occurred at mean buoyant densities of 1.36 and 1.31 g/ml, respectively.
For both feline panleucopaenia and mink enteritis viruses, sedimentation coefficients of DNA from the three density levels which contain DNA, gave results conforming to those expected for single stranded DNA, whilst buoyant density studies showed the DNA of all three species to have a mean density of 1.722. The calculated molecular weight of the DNA of particles banding at 1.41 g/ml (1.7×106 daltons) represents 28.5 per cent of the total particle weight (5.9×106 daltons).
Viral protein can be separated into two major polypeptides with molecular weights of 60,300 and 73,100, amounting to 86 and about 10 per cent, respectively, of the total radioactive amino acids incorporated. A third minor component of molecular weight 39,600 daltons was present at concentrations between 3 and 6 per cent.
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References
Bredis, C. L. Berwick, andT. E. Anderson: Fractionation of Shope papilloma virus in caesium chloride density gradients. Virology17, 84–94 (1962).
Burger, D., J. R. Gorham, andR. L. Ott: Protection of cats against feline panleucopaenia following mink enteritis vaccination. Small Anim. Clinician3, 611–617 (1963).
Gautschi, M., andG. Siegl: Structural proteins of parvovirus Lu III. Arch. ges. Virusforsch.43, 326–333 (1973).
Hallauer, C., andG. Kronauer: Extraction of cell-associated virus without damage of the culture. Arch. ges. Virusforsch.15, 434–440 (1965).
Johnson, R. H.: Isolation of a virus from a condition simulating feline panleucopaenia in a leopard. Vet. Rec.76, 1008–1012 (1964).
Johnson, R. H.: Cytopathic effect of feline panleucopaenia virus. Res. Vet. Sci.6, 472–480 (1965).
Johnson, R. H.: Feline panleucopaenia virus—in vitro comparison of strains with a mink enteritis virus. J. small Anim. Pract.8, 319–323 (1967).
Johnson, R. H.: Serological procedures for the study of feline panleucopaenia. J. Amer. vet. med. Ass.158, 876–880 (1971).
Johnson, R. H., andJ. G. Cruickshank: Problems in classification of feline panleucopaenia virus. Nature (Lond.)212, 622–623 (1966).
Kääriäinen, L., J. Kangas, S. Keränen, M. Nyholm, andP. Weckström: Studies on mink enteritis virus. Arch. ges. Virusforsch.19, 197–209 (1966).
King, D. A., andD. L. Croghan: Immunofluorescence of feline panleucopaenia virus in cell culture. Canad. J. comp. Med.29, 85–89 (1965).
O'reilly, K.: Determination of an optimal dilution of virulent feline infectious enteritis (panleucopaenia) virus for challenge purposes. J. Hyg., Camb.68, 549 to 556 (1970).
Siegl, G., C. Hallauer, A. Novak, andG. Kronauer: Parvoviruses as contaminants of permanent human cell lines. II. Physicochemical properties of the isolated viruses. Arch. ges. Virusforsch.35, 91–103 (1971).
Siegl, G.: Physicochemical characteristics of the DNA of parvovirus Lu III. Arch. ges. Virusforsch.43, 334–344 (1973).
Studdert, M. J., andJ. E. Peterson: Some properties of feline panleucopaenia virus. Arch. ges. Virusforsch.42, 346–354 (1973).
Studier, F. W.: Sedimentation studies of the size and the shape of DNA. J. molec. Biol.11, 373–390 (1965).
Tattersall, P., L. V. Crawford, andA. J. Shatkin: Replication of the parvovirus MVM. II. Isolation and characterization of intermediates in the replication of the viral deoxyribonucleic acid. J. Virol.12, 1446–1456 (1973).
Wildy, P.: Classification and nomenclature of viruses. Monographs in Virology5, 41 (1971).
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On leave from the Department of Tropical Veterinary Science, James Cook University of North Queensland, Townsville, Australia.
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Johnson, R.H., Siegl, G. & Gautschi, M. Characteristics of feline panleucopaenia virus strains enabling definitive classification as parvoviruses. Archiv f Virusforschung 46, 315–324 (1974). https://doi.org/10.1007/BF01240073
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DOI: https://doi.org/10.1007/BF01240073