Abstract
Production of exocellular β-mannan- and xylan-degrading enzymes by eight wood rotting fungi was studied. Although all organisms excreted β-mannanase, endoxyfanase and acetylxylan esterase, production ofl-α-arabinosidase and 4-O-methylglucuronidase was variable. β-Mannanosidase was not detected in any culture filltrate. Righest β-mannanase and endoxylanase activities were observed in cultures ofPolyporus versicolor andSchizophyllum commune grown in Avicel-supplemented media. While crude β-mannanases fromLinzites saepiria andS. commune exhibited equivalent affinities for gluco- and galactomannan substrates,P. versicolor β-mannanase preferred a glucomannan substrate and did not use galactomannan from guar sum as a substrate.
Résumé
On a étudié la production de β-mannanases et de xylanases exo-cellulaires chez huit moisissures pourrissant le bois. Bien que tous les organismes excrètent la β-mannanase, l'endoxylanase et l'esterase de l'acétylxylane, la production del-α-arabinosidase et de la 4-O-méthyl-glucuronisade a été variable. La β-mannanoxidase n'a été détectée dans aucun filtrat de culture. Les activilés les plus élevées en β-mannanase et en endoxylanase ont été observées dans des cultures dePolyporus varsicolor et deSchizaphyllum commune, développées en milleu supplémenté en Avicel. Alors que les β-mannanases brutes deLinzites saepiria et deS. commune ont montré des affinités équivalentes pour les substrats gluco- et galacto-mannanes, la β-mannanase deP. versicolor préfère un substrat gluco-mannane et n'a pas utillisé le galacto-mannane de la gomme guar comme substrat.
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Johnson, K.G. Exocellular β-mannanases from hemicellulolytic fungi. World J Microbiol Biotechnol 6, 209–217 (1990). https://doi.org/10.1007/BF01200943
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DOI: https://doi.org/10.1007/BF01200943