Summary
The temporal and spatial expression of myelin basic protein in the first and second trimester human foetal spinal cord and brainstem from 9 to 20 gestational weeks was determined by immunocytochemistry in sections of cervical, thoracic and lumbosacral levels from 41 human foetal spinal cords and ten brainstems. Myelin basic protein-positive oligodendrocytes were observed peripheral to the ependyma at 9–10 gestational weeks. Oligodendrocytes expressing myelin basic protein were seen at 10–12 gestational weeks in the anterior and lateral funiculi. Myelin basic protein was detected later in the posterior funiculi than in the anterolateral white matter and most spinal cord tracts could not be identified by means of variation in myelin basic protein expression. Mylein basic protein was found in the midline of the brainstem at ten gestational weeks and spread laterally during the second trimester. We conclude that in the human foetal spinal cord, myelin basic protein is present by 10 gestational weeks in the anterolateral cervical spinal cord and midline of the brainstem. It is expressed in a rostral-to-caudal and anterolateral-to-posterior manner in most tracts of the spinal cord. However, an exception to these findings is that the fasciculus gracilis, upon developing into a defined region, had more myelin basic protein-positive cells at the lumbar level than in more rostral regions. Definition of the kinetics of myelin basic protein expression in the normal human foetal spinal cord provides a baseline for study of aberrant myelination and demyelination.
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Barlow, R. M. (1969) The foetal sheep: morphogenesis of the nervous system and histochemical aspects of myelination.Journal of Comparative Neurology,135, 249–62.
Bensted, J. P. M., Dobbing, J., Morgan, R. S., Reid, R. T. W. &Wright, G. P. (1957) Neuroglial development and myelination in the spinal cord of the chick embryo.Journal of Embryology and Experimental Morphology,5, 428–37.
Choi, B. H. (1981) Radial glia of developing human fetal spinal cord: Golgi, immunohistochemical and electron microscopic study.Developmental Brain Research 1, 249–67.
Choi, B. H. &Kim, R. C. (1984) Expression of glial fibrillary acidic protein in immature oligodendroglia.Science 223, 407–9.
Choi, B. H., &Kim, R. C. (1985) Expression of glial fibrillary acidic protein by immature oligodendroglia and its implications.Journal of Neuroimmunology 8, 215–35.
Gamble, H. J. (1969) Electron microscope observations on the human foetal and embryonic spinal cord.Journal of Anatomy 104, 435–53.
Gilles, F. H. (1976) Myelination in the neonatal brain.Human Pathology 7, 244–48.
Gilles, F. H., Shankle, W. &Dooling, E. C. (1983) Myelinated tracts: growth patterns. InThe developing human brain (edited byGilles, F. H., Leviton, A. &Dooling, E. C.), pp. 117–83. Boston: John Wright.
Giulian, D., Johnson, B., Krebs, J. F., Tapscott, M. J. &Honda, S. (1991) A growth factor from neuronal cell lines stimulates myelin protein synthesis in mammalian brain.Journal of Neuroscience 11, 327–36.
Hern, W. M. (1984) Correlation of fetal age and measurements between 10 and 26 weeks of gestation.Obstetrics and Gynecology 63, 26–32.
Jordan, C., Friedrich, V., Jr. &Duboisdalcq, M. (1989)In situ hybridization analysis of myelin gene transcripts in developing mouse spinal cord.Journal of Neuroscience 9, 248–57.
Langworthy, O. R. (1928) The behavior of pouch-young opossums correlated with the myelinization of tracts in the nervous system.Journal of Comparative Neurology 46, 201–47.
Langworthy, O. R. (1933) Development of behavior patterns and myelinization of the nervous system in the human fetus and infant.Contributions to Embryology 24, 3–57.
Levine, S. M. &Goldman, J. E. (1988a) Embryonic divergence of oligodendrocyte and astrocyte lineages in developing rat cerebrum.Journal of Neuroscience 8, 3992–4006.
Levine, S. M. &Goldman, J. E. (1988b) Spatial and temporal patterns of oligodendrocyte differentiation in rat cerebrum and cerebellum.Journal of Comparative Neurology 277, 441–55.
Lucas Keene, M. F. &Hewer, E. E. (1931) The development and myelination of the posterior longitudinal bundle in the human.Journal of Anatomy 67, 522–35.
Polak, J. M. &Van Noorden, S. eds. (1983)Immunocytochemistry. Practical applications in pathology and biology. Bristol: John Wright and Sons Ltd.
Romanes, G. J. (1947) The prenatal medullation of the sheep's nervous system.Journal of Anatomy 81, 64–81.
Rorke, L. B. &Riggs, H. E. (1969)Myelination of the brain in the newborn, pp. 26–8. Philadelphia: J.B. Lippincott Co.
Rozeik, C. &Von Keyserlingk, D. (1987) The sequence of myelination in the brainstem of the rat monitored by myelin basic protein immunohistochemistry.Developmental Brain Research 35, 183–90.
Sasaki, A., Hirato, J., Nakazato, Y. &Ishida, Y. (1988) Immunohistochemical study of the early human fetal brain.Acta Neuropathologica 76, 128–34.
Schwab, M. E. &Schnell, L. (1989) Region-specific appearance of myelin constituents in the developing rat spinal cord.Journal of Neurocytology 18, 161–9.
Sternberger, N. H., Itoyama, Y., Kies, M. W. &Webster, H. Def. (1978) Myelin basic protein demonstrated immunocytochemically in oligodendroglia prior to myelin sheath formation.Proceedings of the National Academy of Sciences (USA) 75, 2521–4.
Tilney, F. &Casamajor, L. (1924) Myelinogeny as applied to the study of behavior.Archives of Neurology and Psychiatry 12, 1–66.
Tohyama, T., Lee, V. M.-Y., Rorke, L. B., &Trojanowski, J. Q. (1991) Molecular milestones that signal axonal maturation and the commitment of human spinal cord precursor cells to the neuronal or glial phenotype in development.Journal of Comparative Neurology 310, 1–15.
Warf, B. C., Fok-Seang, J. &Miller, R. H. (1991) Evidence for the ventral origin of oligodendrocyte precursors in the rat spinal cord.Journal of Neuroscience 11, 2477–88.
Weidenheim, K. M., Kress, Y., Epshteyn, I., Rashbaum, W. K. &Lyman, W. D. (1992) Early myelination in the human fetal lumbosacral spinal cord: characterization by light and electron microscopy.Journal of Neuropathology and Experimental Neurology 51, 142–9.
Yakovlev, P. I. &Lecours, A-R. (1967) The myelogenetic cycles of regional maturation of the brain. InRegional development of the brain in early life (edited byMinkowski, A.), pp. 3–70. Philadelphia: F. A. Davis Co.
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Weidenheim, K.M., Epshteyn, I., Rashbaum, W.K. et al. Neuroanatomical localization of myelin basic protein in the late first and early second trimester human foetal spinal cord and brainstem. J Neurocytol 22, 507–516 (1993). https://doi.org/10.1007/BF01189039
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DOI: https://doi.org/10.1007/BF01189039