Abstract
The hemocytic and gill tissue responses of the crabParatelphusa hydrodromous (Herbst) to a wide range of sublethal concentrations of cadmium chloride (0.02–0.50 μM/L) were examined after a 30-day exposure using hemocyte counts (THC and DHC) and lamellar pathology. A continued reduction in the hemocyte counts and selective changes in the numbers of hyalinocytes and eosinophilic granulocytes was evident in the toxified crabs. Under sublethal stress, the hyalinocytes developed eccentric nuclei, granular cytoplasm and membrane blebs. Atypical shape, lobate nucleus, dense cytoplasmic deposits and granuloplasmic vacuoles were frequently observed in the granulocytes. Greater proliferation of prohemocytes and abnormal hemocyte morphology indicated cadmium-induced neoplastic transformation of hemocytopoietic organs. No major structural changes in the gills were noted at 0.02 μM CdCl2. Nodular gill disease (NGD), hemocytic hyperplasia and sloughing of walled off hemocytes were prominant lesions after 0.50 μM cadmium chloride exposure.
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References
Ahsanullah M (1976) Acute toxicity of cadmium and zinc to seven invertebrate species from Western Port Victoria. Aust J Mar Freshwater Res 27:187–196
Ahsanullah M, Arnott GH (1978) Acute toxicity of copper, cadmium and zinc to larvae of the crabParagrapsus quadridentatus (H. Milne Edwards) and implications for water quality criteria. Aust J Mar Freshwater Res 29:1–8
Anilkumar C, Adiyodi KG (1980) Ovarian growth, induced by eyestalk ablation during the prebreeding season is not normal in the crabParatelphusa hydrodromous (Herbst). Int J Invertebr Reprod 2:95–105
Beaulaton J (1968) Etude ultrastructural et cytochimie des glandes prothoraciques de vers a soie aux quatrieme et cinquieme ages larvaires I. La tunica propria et ses relations avec les fibres conjonctives et les hemocytes. J Ultrastruct Res 23:474–498
Collier RS, Miller JE, Dawson MA, Thurberg FB (1978) Physiological responses of the mud crabEurypanopeus depressus to cadmium. Bull Environ Contam Toxicol 10:378–382
Committee on Methods for Toxicity tests with Aquatic Organisms (1975) Methods for acute toxicity tests with fish, macroinvertebrates and amphibians. National Water Quality Laboratory, Duluth, Minnesota, USEPA-660/3-75-009
Corner EDS, Rigler FH (1958) The modes of action of toxic agents III. Mercuric chloride and n-amylmercuric chloride on crustaceans. J Mar Biol Assoc UK 37:85–96
Cornick JW, Stewart JE (1968) Interaction of the pathogen Gaffkya homari with natural defense mechanisms ofHomarus americanus. J Fish Res Board Canada 25:695–709
Cornick JW, Stewart JS (1978) Lobster (Homarus americanus) hemocytes: classification, differential counts and associated agglutinin activity. J Invertebr Pathol 31:194–203
Couch JA (1978) Diseases, Parasites and toxic responses of commercial penaeid shrimps of the Gulf of Mexico and south Atlantic coasts of North America. Fish Bull 76:1–44
Deruby KG (1918) A study on autolysis of animal tissues. J Biol Chem 35:179–219
Dumont JN, Anderson E, Winner G (1966) Some cytological characteristics of the hemocytes ofLimulus during clotting. J Morphol 119:181–208
Eisler R (1971) Cadmium poisoning inFundulus heteroclitus (Pisces: Cyprinodontidae) and other marine organisms. J Fish Res Board Canada 28:1225–1234
Eisler R (1974) Radiocadmium exchange with seawater byFundulus heteroclitus (L) (Pisces: Cyprinodontidae). J Fish Biol 6:601–612
Esterlla BT (1984) Black gill and shell disease in American lobster (Homarus americanus) as indicators of pollution in Massachusetts Bay and Buzzards Bay, Massachusetts. Mass Div Mar Fish Pub No 14049-19-125-5-85-CR, 17 pp
Finney DJ (1971) Probit analysis. Cambridge University Press, London
Fontaine CT, Lightner DV (1975) Cellular response to injury in penaeid shrimp. Mar Fish Rev 37(5-6):1–10
Greig RA, Sawyer TK, Lewis EJ, Galasso ME (1982) A study of metal concentrations in relation to gill color and pathology in the rock crab. Arch Environ Contam Toxicol 11:539–545
Jennings JR, Rainbow PS (1969) Studies on the uptake of cadmium by the crabCarcinus maenas in the laboratory. I. Accumulation from seawater and a food source. Mar Biol 50:131–139
Lai-Fook J (1970) Haemocytes in the repair of wounds in an insect (Rhodnius prolixus). J Morphol 130:297–313
Levin J (1967) Blood coagulation and endotoxin in invertebrates. Fed Proc 26:1707–1712
Martin GG, Graves BL (1985) Fine structure and Classification of shrimp hemocytes. J Morphol 185:339–348
Mix MC, Sparks AK (1980) Hemocyte classification and differential counts in the dungeness crabCancer magister. J Invertebr Pathol 35:134–143
Myers DK, DeWolfe-Glade D (1964) Some effects of cationic condensing agents on rat thymocyte. Exp Cell Res 33:344–349
Newman MC, Feng SY (1982) Susceptibility and resistance of the rock crabCancer irroratus to natural and experimental bacterial infection. J Invertebr Pathol 40:75–88
Nimmo DR, Lightner DV, Bahner LH (1977) Effects of cadmium on the shrimpPenaeus duorarum, Palaemonetes pugio, Palaemonetes vulgaris. In: Vernberg FJ, Calabrese A, Thurberg FP, Vernberg WB (eds) Physiological responses of marine biota to pollutants. Academic Press, New York, p 131
Papathanassiou E, King PE (1883) Ultrastructural studies on the gills ofPalaemon serratus (Pennant) in relation to cadmium accumulation. Aquat Toxicol 3:273–284
Rabin H (1970) Hemocytes, hemolymph and defense reactions in crustaceans. J Reticuloendoth Soc 7:195–207
Rainbow PS, White SL (1989) Comparative strategies of heavy accumulation by crustaceans: Zinc, copper and cadmium in a decapod, an amphipod and a barnacle. Hydrobiologia 174:245–262
Stewart JE, Cornick JW and Dingle JR (1967) An electronic method for counting lobster (Homarus americanus Milne Edwards) hemocytes and the influence of diet on hemocyte numbers and hemolymph proteins. Can J Zool 45:291–304
Stewart JE, Arie B, Zwicker BM, Dingle JR (1969) Gaffkemia a bacterial disease of the lobsterHomarus americanus: effects of the pathogenGaffkya homari on the physiology of the host. Can J Microbiol 15:925–932
Sindermann CJ (1971) Internal defenses of Crustacea: A review. Fish Bull US 69:455–489
Smith VJ, Soderhall K (1983) B-1,3 glucan activation of crustacean hemocytein vitro andin vivo. Biol Bull 164:299–314
Smith VJ, Soderhall K, Hamilton M (1984) B, 1,3-Glucon induced cellular defence reactions in the shore crabCarcinus maenas. Comp Biochem Physiol 77A:635–639
Thorp VJ, Lake PS (1974) Toxicity bioassays of cadmium on selected freshwater invertebrates and the interaction of cadmium and zinc on the freshwater shrimpParatya tasmaniensis Riek. Aust J Mar Freshwater Res 25:97–104
Van Harreveld A (1936) A physiological solution for freshwater crustaceans. Proc Soc Exper Biol Med 34:428–432
White SL, Rainbow PS (1982) Regulation and accumulation of copper, zinc and cadmium by the shrimpPalaemon elegans. Mar Ecol Prog Ser 8:95–101
Wood PJ, Visentin LP (1967) Histological and histochemical observations of the hemolymph cells in the crayfishOrconectes virilis. J Morphol 123:559–568
Wright DA (1977) The effect of salinity on cadmium uptake by the tissues of the shorecrabCarcinus maenas. J Exp Biol 67:137–146
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Victor, B. Responses of hemocytes and gill tissues to sublethal cadmium chloride poisoning in the crabParatelphusa hydrodromous (Herbst). Arch. Environ. Contam. Toxicol. 24, 432–439 (1993). https://doi.org/10.1007/BF01146158
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DOI: https://doi.org/10.1007/BF01146158