Abstract
The efficacy of benzyladenine (BA) to induce multiple shoots from seed explants of chickpea (Cicer arietinum L.) was assessed. Shoot differentiation was influenced by the type of seed explant, genotype and concentration of BA. Orientation of the explant also strongly influenced the shoot regeneration response. The optimum BA concentration for shoot/shoot bud regeneration was genotype dependent. Two types of BA-induced response were observed: (1) at less than 7.5 gm BA, direct shoot differentiation (2 to 4-cm-long shoots) was observed within 30 days; (2) at higher BA concentrations (75–100 μm), shoot/shoot bud differentiation was achieved in 45–90 days. A high BA concentration inhibited subsequent rooting of shoots. Roots, however, could be easily induced on shoots derived from <12.5 μm BA. Following transfer to soil, 80% of the regenerants developed into morphologically normal and fertile plants.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- BA :
-
Benzyladenine
References
Barna KS, Wakhlu AK (1993) Somatic embryogenesis and plant regeneration from callus cultures of chickpea (Cicer arietinum L.). Plant Cell Rep 12:521–524
Brandt EB, Hess D (1994) In vitro regeneration and propagation of chickpea (Cicer arietinum L.) from meristem tips and cotyledonary nodes. In Vitro Cell Dev Biol Plant 30P:75–80
Cheng TY, Saka H, Voqui-Dinh TH (1980) Plant regeneration from soybean cotyledonary node segments in culture. Plant Sci Lett 19:91–99
Franklin CI, Trieu TN, Gongales RA, Dixon RA (1991) Plant regeneration from seedling explants of green bean (Phaseolus vulgaris L.) via organogenesis. Plant Cell Tissue Organ Cult 24:199–206
Gambley RL, Dodd WA (1990) An in vitro technique for the production de novo of multiple shoots in cotyledon explants of cucumber (Cucumis sativus L.). Plant Cell Tissue Organ Cult 20:177–183
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements for suspension cultures of soybean root cells. Exp Cell Res 50:151–158
Gill R, Eapen S, Rao PS (1987) Morphogenic studies of cultured cotyledons of urd bean (Vigna mungo L. Hepper). J Plant Physiol 130:1–5
Gulati A, Jaiwal PK (1990) Culture conditions affecting plant regeneration from cotyledon of mungbean (Vigna radiata L. Wilczek). Plant Cell Tissue Organ Cult 23:1–7
Gulati A, Jaiwal PK (1994) Plant regeneration from cotyledonary node explants of mungbean (Vigna radiata L. Wilczek). Plant Cell Rep 13:523–527
Malik KA, Saxena PK (1992a) Regeneration inPhaseolus vulgaris L. High frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron. Planta 186:384–389
Malik KA, Saxena PK (1992b) Thidiazuron induces high frequency shoot regeneration in intact seedlings of pea (Pisum sativum), chickpea (Cicer arietinum) and lentil (Lens culinaris). Aust J Plant Physiol 19:731–740
Mathews H (1987) Morphogenetic responses for in vitro cultured seedling explants of mung bean (Vigna radiata L. Wilczek). Plant Cell Tissue Organ Cult 11:163–168
McClean P, Grafton KF (1989) Regeneration of dry bean (Phaseolus vulgaris L.) via organogenesis. Plant Sci 60:117–122
McKently AH, Moore GA, Gardner FP (1989) In vitro plant regeneration of peanut from seed explants. Crop Sci 30:192–196
Mehta U, Mohan Ram HY (1980) Regeneration of plantlets from cotyledons ofCajanus cajan. Ind J Exp Biol 18:800–802
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Polisetty R, Patil P, Deveshwar JJ, Khetarpal S, Chandra R (1996) Rooting and establishment of in vitro grown shoot tip explants of chickpea (Cicer arietinum L.). Ind J Exp Biol 34:806–809
Prakash NS, Pental D, Sarin NB (1994) Regeneration of pigeonpea (Cajanus cajan) from cotyledonary node via multiple shoot formation. Plant Cell Rep 13:623–627
Rubluo A, Kartha KK (1985) In vitro culture of shoot apical meristem of variousPhaseolus species and cultivars. J Plant Physiol 119:425–433
Author information
Authors and Affiliations
Additional information
Communicated by G. Philipps
Rights and permissions
About this article
Cite this article
Polisetty, R., Paul, V., Deveshwar, J.J. et al. Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chickpea (Cicer arietinum L.). Plant Cell Reports 16, 565–571 (1997). https://doi.org/10.1007/BF01142325
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01142325