Summary
Aerobic cultures harvested from the lag and early exponential growth phases fermented D-xylose poorly under anaerobic conditions whereas fermentation by late exponential and stationary phase cultures was rapid. These differences could be related to the ratios of NADH- to NADPH-linked xylose reductase (XR) and the levels of NADH-linked XR and NAD-linked xylitol dehydrogenase (XD) present. Under aerobic conditions, induction of NADPH-linked XR preceded NADH-linked XR which suggested the presence of two separate XR't's. Induction of XR and XD was more rapid under aerobic than anaerobic conditions.
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Bolen, P.L., Roth, K.A., and Freer, S.N. (1986)Appl. Environ. Microbiol. 52, 660–664.
Bruinenberg, P.M., De Bot, P.H.M., Van Dijken, J.P., and Scheffers, W.A. (1983a)Eur. J. Appl. Microbiol. Biotechnol. 18, 287–292.
Bruinenberg P.M., De Bot, P.H.M., Van Dijken, J.P., and Scheffers, W.A. (1984)Appl. Microbiol. Biotechnol. 19, 256–260.
Ditzelmüller, G., Kubicek, C.P., Wöhrer, W., and Röhr, M. (1984a)FEMS Microbiol. Lett. 25, 195–198.
Ditzelmüller, G., Kubicek, C.P., Wöhrer, W., and Röhr, M. (1984b)Can. J. Microbiol. 30, 1330–1336.
Ditzelmüller, G., Kubicek-Pranz, E.M., Röhr, M., and Kubicek, C.P. (1985)Appl. Microbiol. Biotechnol. 22, 297–299.
Du Preez, J.C., Prior, B.A., and Monteiro, A.M.T. (1984)Appl. Microbiol. Biotechnol. 19, 261–266.
Hungate, R.E. (1986) InMethods in Microbiol., J.R. Norris and D.W. Ribbons eds., vol 3B, pp 117–132.
Jeffries, T.W. (1982)Biotechnol. Bioeng. Symp. 12, 103–110
Jeffries, T.W. (1985)Trends Biotechnol. 3, 208–212.
Ligthelm, M.E., Prior, B.A., and Du Preez, J.C. (1988)Appl. Microbiol. Biotechnol. (in print).
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951)J. Biol. Chem. 193, 265–275.
Morimoto, S., Matsuo, M., Azuma, K., and Sinskey, A.J. (1986)J. Ferment. Technol. 64, 219–225.
Neirinck, L., Maleszka, R., and Schneider, H. (1984)Arch. Biochem. Biophys. 228, 13–21.
Schneider, H., Wang, P.Y., Chan, Y.K., and Maleszka, R. (1981)Biotechnol. Lett. 3, 89–92.
Schneider, H., Maleszka, R., Neirinck, L., Veliky, I.A., Wang, P.Y., and Chan, Y.K. (1983)Adv. Biochem. Eng./Biotechnol. 27, 57–71.
Schvester, P., Robinson, C.W., and Moo-Young, M. (1983)Biotechnol. Bioeng. Symp. 13, 131–152.
Slininger, P.J., Bothast, R.J., Van Cauwenberge, J.E., and Kurtzman, C.P. (1982a)Biotechnol. Bioeng. 24, 371–384.
Slininger, P.J., Bothast, R.J., Black, L.T., and McGhee, J.E. (1982b)Biotechnol. Bioeng. 24, 2241–2251.
Smiley, K.L., and Bolen, P.L. (1982)Biotechnol. Lett. 4, 607–610.
Suihko, M.-L., Drazić, M. (1983)Biotechnol. Lett. 5, 107–112.
Van Dijken, J.P., and Scheffers, W.A. (1986)FEMS. Microbiol Rev. 32, 199–224.
Verduyn, C., Jzn, J.F., Van Dijken, J.P., and Scheffers, W.A. (1985)FEMS Microbiol. Lett. 30, 313–317.
Watson, N.E., Prior, B.A., Du Preez, J.C., and Lategan, P.M. (1984)Enzyme Microb. Technol. 6, 447–450.
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Ligthelm, M.E., Prior, B.A. & du Preez, J.C. The induction of D-xylose catabolizing enzymes inPachysolen tannophilus and the relationship to anaerobic D-xylose fermentation. Biotechnol Lett 10, 207–212 (1988). https://doi.org/10.1007/BF01134831
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DOI: https://doi.org/10.1007/BF01134831