Abstract
Human bone cells, maintained in culture, have been subjected to partitioning in an aqueous two-phase system on a countercurrent distribution apparatus. A broad cell distribution was obtained indicating cell-surface heterogeneity. Two major cell populations were identified which appeared to be growing at different rates. The ‘fast’-growing cells had a less hydrophobic cell surface than the ‘slow’-growing cells. Possible relationships of these cell populations with osteoblast differentiation and the potential importance of this technique in studies of osteoblast differentiation are discussed.
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Sharpe, P.T., MacDonald, B.R., Gallagher, J.A. et al. Studies of the growth of human bone-derived cells in culture using aqueous two-phase partition. Biosci Rep 4, 415–419 (1984). https://doi.org/10.1007/BF01122506
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DOI: https://doi.org/10.1007/BF01122506