Abstract
Patch-clamp recordings from ventricular myocytes of neonatal rats identified ionic channels that open in response to membrane stretch caused by negative pressures (1 to 6 cm Hg) in the electrode. The stretch response, consisting of markedly increased channel opening frequency, was maintained, with some variability, during long (>40 seconds) stretch applications. The channels have a conductance averaging 120 pS in isotonic KCl, have a mean reversal potential 31 mV depolarized from resting membrane potential, and do not require external Ca++ for activation. The channels appear to be relatively non-selective for cations. Since they are gated by physiological levels of tension, stretch-activated channels may represent, a cellular control system wherein beat-to-beat tension and/or osmotic balance modulate a portion of membrane conductance.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- SACs:
-
stretch-activated channels
- HEPES:
-
4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid
References
Ahumada, G. G., Sobel, B. E. and Needleman, P. (1980) The synthesis of prostaglandins by cultured rat heart myocytes and cardiac mesenchymal cells.J. Mol. Cell. Cardiol. 12:685–700.
Allen, D. G. (1977) On the relationship between action potential duration and tension in cat papillary muscle.Cardiovasc. Res. 11:210–218.
Balzer, J. R. and Roden, D. M. (1988) Lanthanum sensitive current contaminates Ik in guinea pig ventricular myocytes.Biophys. J. 53:642a (abstract).
Brehm, P., Kuhlberg, R. and Moody-Corbett, F. (1984) Properties of non-junctional acetylcholine receptor channels on innervated muscle of Xenopus larvaeJ. Physiol. Lond. 350:631–648.
Brezden, B. L., Gardner, D. R. and Morris, C. E. (1985) A potassium-selective channel in isolated lymnaea stagnalis heart muscle cells.J. Exp. Biol. 123:175–189.
Christensen, O. (1987) Mediation of cell volume regulation by Ca++ influx, through stretchactivated channels.Nature 330:66–68.
Clay, J. R. and Shrier, A. (1981) Analysis of subthreshold pacemaker currents in chick embryonic heart cells.J. Physiol. 312:491–504.
Cooper, K. E., Tang, J. M., Rae, J. L. and Eisenberg, R. S. (1986) A cation channel in frog lens epithelia responsive to pressure and calcium.J. Memb. Biol. 93:259–269.
Corey, D. P. and Hudspeth, A. J. (1979) Ionic basis of receptor potential in the vertebrate hair cell.Nature 281:675–677.
DiFrancesco, D. (1985) The cardiac hyperpolarizing-activated current,I f, origins and development.Prog. Biophys. Molec. Biol. 46:163–183.
Grigg, P. (1986) Biophysical studies of mechanoreceptorsJ. Appl. Physiol. 60:1107–1115.
Guhary, F. and Sachs, F. (1984) Stretch-activated single ion channel currents in tissue culture embryonic chick skeletal muscle.J. Physiol. Lond. 352:685–701.
Hamill, O. P., Marty, A., Neher, E., Sakmann, B., Sigworth, F. J. (1981). Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.Pflugers Arch. ges. Physiol. 391:85–100.
Hamill, O. P. (1983) Potassium and chloride channels in red blood cells. In:Single Channel Recording, Sakmann, B., Neeher, E., Eds Plenum, 451–471.
Josephson, I. R., Sanchey-Chapula, J. and Brown, A. M. (1984) A comparison of calcium currents in rat, in guinea pig single ventricular cells.Circ. Res. 54:144–156.
Kullberg, R. (1987) Stretch-activated ion channels in bacteria and animal cell membranes.Trends in Neurosci. 10:387–388.
Lansman, J. B., Hallam, T. J. and Rink, T. J. (1987) Single stretch-activated ion channels in vascular endothelium cells as mechanotransducers?Nature 325:811–813.
Linden, R. J. (1976) Reflexes from receptors in the heart.Cardiol. 61:7–30.
Martinac, B., Buechner, M., Delcour, A. H., Adler, J. and Kung, C. (1987) Pressure sensitive ion channels inE. Coli.Proc. Natl. Acad. Sci. USA 84:2297–2301.
McGaughey, M. D., Maughan, W. L., Sunagawa, K. and Sagawa, K. (1985) Alternating contractility in pulsus alternans studied in the isolated canine heart.Circ. 71:357–362.
Nakayayama, T., Kuraki, Y., Noma, A. and Irisawa, H. (1984) Action potential and membrane currents of single pacemaker cells of the rabbit heart,Pflugers Arch. 402:248–257.
Rajala, G. M., Pinter, M. J. and Kaplan, S. (1977) Response of the quiescent heart tube to mechanical stretch in the intact chick embryo.Develop. Biol. 61:330–337
Sachs, F. (1986) Biophysics of mechanoreception. In:Membrane Bioch.,6:173–195.
Sackin, H. (1987) Stretch-activated potassium channels in renal proximal tubule.Am. J. Physiol. 253:F1253-F1262.
Sakmann, B., Methfessel, C., MIshina, M., takahashi, T., Takai, T., Masaaki, K., Fukuda, K. and Numa, S. (1985) Role of acetylcholine receptor sub-units in gating of the channel.Nature 318, 538–540.
Sigurdson, W. J., Morris, C. E., Brezden, B. L. and Gardner, D. R. (1986) Stretch activation of a K+ channel in molluscan heart cells.J. Exp. Biol. 127:191–209.
Yang, X. C. and Sachs, F. (1988) Stretch-activated (SA) ion channel in Xenopus oocytes: Permeation and block.Biophys. J. 53:412a (abstract).
Zucker, I. H. (1986) Left-ventricular receptors: Physiological controllers or pathological curiosities?Basic Res. Cardiol. 8:539–557.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Craelius, W., Chen, V. & El-Sherif, N. Stretch activated ion channels in ventricular myocytes. Biosci Rep 8, 407–414 (1988). https://doi.org/10.1007/BF01121637
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01121637