Summary
-
1.
Multiple subunits of hemocyanins from 3 brachyuran crabs (Carcinus maenas, Cancer pagurus, Hyas araneus), a spiny lobster (Palinurus vulgaris), a freshwater crayfish (Astacus leptodactylus), and a lobster (Homarus americanus) were isolated by preparative polyacrylamide gel electrophoresis (PAGE), and compared by two-dimensional immunoelectrophoresis.
-
2.
In the 24 S hemocyanin isolated from each of the 3 crabs, two of the four subunits separated are immunologically identical; the third subunit is antigenically deficient compared to the first two. The fourth chain is immunologically unrelated to the other three.
-
3.
In the 16 S hemocyanin ofPalinurus two of the four subunits are immunologically identical; the third is closely related. The fourth chain is partially identical with the other three, but is antigenically deficient.
-
4.
The 16 S hemocyanin ofAstacus is composed of two immunologically unrelated subunits. The 24 S hemocyanin of this species contains, in addition, a dimeric subunit which is partially identical with one of the former, but not related to the other.
-
5.
In the 24 S hemocyanin fromHomarus, five subunits were separated; a group of 2, and a group of 3 subunits which are immunologically identical, but which are not related to each other.
-
6.
At the level of quaternary structure, a common principle is suggested for crustacean hemocyanins: 24 S hemocyanins are composed of three types of subunits, while 16 S hemocyanins contain only two types.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Baty D, Lazdunski C (1979) An anti-(signal peptide) antibody: purification, properties and use as a conformational probe. Eur J Biochem 102:503–507
Berg AA Van den, Gaastra W, Kuiper HA (1977) Heterogeneity ofPanulirus interruptus hemocyanin. In: Bannister JV (ed) Structure and function of haemocyanin. Springer, Berlin Heidelberg New York, pp 6–12
Bijlholt MMC, Bruggen EFJ van, Bonaventura J (1979) Dissociation and reassembly ofLimulus polyphemus hemocyanin. Eur J Biochem 95:399–405
Bock E, Axelsen NH (1973) Comparison of antigens: The reaction of partial identity. Scand J Immunol 2, Suppl 1 95–99
Crumpton MJ (1974) Protein antigens. In: Sela M (ed) The antigens vol II. Academic Press, Oxford, pp 1–79
Eerd JP van, Folkerts A (1981) Isolation and characterization of the five subunits from the hemocyanin of the spiny lobsterPanulirus interruptus. In: Lamy J (ed) Structure, active site, and function of invertebrate oxygen binding proteins. Dekker, New York, in press
Holde KE Van, Bruggen EFJ van (1971) The hemocyanins. In: Timasheff SN, Fasman GD (eds) Subunits in biological systems. Dekker, New York, pp 1–53
Hoylaerts M, Préaux G, Witters R, Lontie R (1979) Immunological heterogeneity of the subunits ofLimulus polyphemus haemocyanin. Arch Int Physiol Biochim 87:417–418
Krøll J (1973a) Tandem-crossed immunoelectrophoresis. Scand J Immunol 2, Suppl 1:57–59
Krøll J (1973b) Crossed-line immunoelectrophoresis. Scand J Immunol 2, Suppl 1:79–81
Lamy J, Lamy J, Weill J (1979a) Arthropod hemocyanin structure. Isolation of eight subunits in the scorpion. Arch Biochem Biophys 193:140–149
Lamy J, Lamy J, Weill J, Markl J, Schneider H-J, Linzen B (1979b) Hemocyanins in spiders. VII. Immunological comparison of the subunits ofEurypelma californicum hemocyanin. Hoppe-Seyler's Z Physiol Chem 360:889–895
Lamy J, Lamy J, Weill J, Bonaventura J, Bonaventura C, Brenowitz M (1979c) Purification and immunological identification of the dissociation products of nativeLimulus polyphemus andTachypleus tridentatus hemocyanins. Arch Biochem Biophys 196:324–339
Lamy J, Lamy J, Sizaret P-Y, Weill J (1981) Quaternary structure ofAndroctonus australis hemocyanin. In: Lamy J (ed) Structure, active site, and function of invertebrate oxygen binding proteins. Dekker, New York, in press
Markl J, Markl A, Schartau W, Linzen B (1979a) Subunit heterogeneity in arthropod hemocyanins: I. Chelicerata. J Comp Physiol 130:283–292
Markl J, Hofer A, Bauer G, Markl A, Kempter B, Brenzinger M, Linzen B (1979b) Subunit heterogeneity in arthropod hemocyanins: II. Crustacea. J Comp Physiol 133:167–175
Markl J, Strych W, Schartau W, Schneider H-J, Schöberl P, Linzen B (1979c) Hemocyanins in spiders. VI. Comparison of the polypeptide chains ofEurypelma californicum hemocyanin. Hoppe-Seyler's Z Physiol Chem 360:639–650
Markl J, Kempter B (1981) Subunit heterogeneity in arthropod hemocyanins. In: Lamy J (ed) Structure, active site, and function of invertebrate oxygen binding proteins. Dekker, New York, in press
Markl J, Decker H, Savel A, Linzen B (1981) Homogeneity, subunit heterogeneity, and quaternary structure ofEurypelma hemocyanin. In: Lamy J (ed) Structure, active site, and function of invertebrate oxygen binding proteins. Dekker, New York, in press
Midelfort CF, Mehler AH (1972) Deamination in vivo of an asparagine residue of rabbit muscle aldolase. Proc Natl Acad Sci USA 69:1816–1819
Murray AC, Jeffrey PD (1974) Hemocyanin from the Australian freshwater crayfish,Cherax destructor. Subunit heterogeneity. Biochemistry 13:3667–3671
Nisonoff A, Reichlin M, Margoliash E (1970) Immunological activity of cytochrome c. J Biol Chem 245:940–946
Ouchterlony Ö (1958) Diffusion-in-gel methods for immunological analysis. Prog Allergy 5:1–78
Ouchterlony Ö (1962) Diffusion-in-gel methods for immunological analysis II. Prog Allergy 6:30–154
Reichlin M, Bucci E, Wymann J, Antonini E, Rossi-Fanelli A (1964) The immunological difference between horse oxy- and deoxyhemoglobin. J Mol Biol 9:785–788
Rochu D, Lambin P, Ghidalia W, Fine JM (1978) Hemocyanin subunits and their polymeric forms in some decapod Crustacea. Comp Biochem Physiol 59B:117–122
Sullivan B, Bonaventura J, Bonaventura C (1974) Functional differences in the multiple hemocyanins of the horseshoe crab,Limulus polyphemus L. Proc Natl Acad Sci USA 71:2558–2562
Sullivan B, Bonaventura J, Bonaventura C, Godette G (1976) Hemocyanin of the horseshoe crab,Limulus polyphemus. Structural differentiation of the isolated components. J Biol Chem 251:7644–7648
Weeke B (1973) Crossed immunoelectrophoresis. Scand J Immunol 2, Suppl 1:47–56
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Markl, J., Kempter, B. Subunit heterogeneity in crustacean hemocyanins as deduced by two-dimensional immunoelectrophoresis. J Comp Physiol B 141, 495–502 (1981). https://doi.org/10.1007/BF01101472
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01101472