Abstract
Five different techniques have been used with sevenPisum (pea) genotypes to demonstrate the existence of extensive genotypic variability in the proportions of the major pea seed globulins, legumin, vicilin and convicilin. The accuracy and reproducibility of each method have been assessed and the significance of the observed variability discussed both in relation to studies of the control of globulin synthesis and to breeding for pea seed protein with higher nutritional quality.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Casey R (1979) Immunoaffinity chromatography as a means of purifying legumin fromPisum (pea) seeds. Biochem J 177:509–520
Casey R (1979) Genetic variability in the structure of the α-subunits of legumin fromPisum — a two-dimensional gel electrophoresis study. Heredity 43:265–272
Casey R, Sanger E (1980) Purification and some properties of a 7S seed storage protein fromPisum (pea). Biochem Soc Trans 8:658
Casey R, Short MN (1981) Variation in amino acid composition of legumin fromPisum. Phytochemistry 20:21–23
Casey R, March JF, Sanger E (1981) N-terminal amino acid sequence of β-subunits of legumin fromPisum sativum. Phytochemistry 20:161–163
Croy RRD, Derbyshire E, Krishna TG, Boulter D (1979) Legumin ofPisum sativum andVicia faba. New Phytol 83:29–35
Croy RRD, Gatehouse JA, Evans IM, Boulter D (1980) Characterisation of the storage protein subunits synthesised in vitro by poly-ribosomes and RNA from developing pea (Pisum sativum L.) I. Legumin. Planta 148:49–56
Croy RRD, Gatehouse JA, Evans IM, Boulter D (1980) Characterisation of the storage protein subunit synthesised in vitro by polyribosomes and RNA from developing pea (Pisum sativum L.) II. Vicilin. Planta 148:57–63
Croy RRD, Gatehouse JA, Tyler M, Boulter D (1980) The purification and characterisation of a third storage protein (convicilin) from the seeds of pea (Pisum sativum L.). Biochem J 191:509–516
Davies DR (1976) Variation in the storage proteins of peas in relation to sulphur amino-acid content. Euphytica 25:717–724
Davies DR (1980) The ra locus and legumin synthesis inPisum sativum. Biochem Genet 18:1207–1219
Derbyshire E, Wright DJ, Boulter D (1976) Legumin and vicilin, storage proteins of legume seeds. Phytochemistry 15:3–24
Domoney C, Davies DR, Casey R (1980) The initation of legumin synthesis in immature embryos ofPisum sativum L. grown in vivo and in vitro. Planta 149:454–460
Evans IM, Croy RRD, Hutchinson P, Boulter D, Payne PI, Gordon ME (1979) Cell free synthesis of some storage protein subunits by polyribosomes and RNA isolated from developing seeds of peas (Pisum sativum L.). Planta 144:455–462
Evans IM, Croy RRD, Brown P, Boulter D (1980) Synthesis of complementary DNAs to partially purified mRNAs coding for the storage proteins ofPisum sativum (L). Biochim Biophys Acta 610:81–95
Gatehouse JA, Croy RRD, Boulter D (1980) Isoelectric-focusing properties and carbohydrate content of pea (Pisum sativum) legumin. Biochem J 185:497–503
Gatehouse JA, Croy RRD, McIntosh R, Paul C, Boulter D (1980) Quantitative and qualitative variation in the storage proteins of material from the EEC joint field bean test. In: Bond DA (ed)Vicia faba; feeding value, processing and viruses. Brussels: EEC, pp 173–190
Grant DR, Lawrence JM (1964) Effects of sodium dodecyl sulfate and other dissociating reagents on the globulins of peas. Arch Biochem Biophys 108:552–561
Guldager P (1978) Immunoelectrophoretic analysis of seed proteins fromPisum sativum L. Theor Appl Genet 53:241–250
Higgins TJV, Spencer D (1977) Cell free synthesis of pea seed proteins. Plant Physiol 60:655–661
Higgins TJV, Spencer D (1981) Precursor forms of pea vicilin subunits. Modification by microsomal membranes during cell-free translation. Plant Physiol 67:205–211
Johnson PJ, Ogston AG (1946) A boundary anomaly found in the ultracentrifugal sedimentation of mixtures. Trans Farad Soc 4:789–799
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277:680–685
Laurell CB (1967) Quantitative estimation of proteins by electrophoresis in antibody-containing agarose gels. In: Peeters H (ed) Proteins in biological fluids, vol 14. Amsterdam: Elsevier, pp 499–502
Millerd A, Thomson JA, Schroeder HE (1978) Cotyledonary storage proteins inPisum sativum. III. Patterns of accumulation during development. Aust J Plant Physiol 5:519–534
Randall PJ, Thomson JA, Schroeder HE (1979) Cotyledonary storage proteins inPisum sativum. IV. Effects of sulfur, phosphorus, potassium and magnesium deficiencies. Aust J Plant Physiol 6:11–24
Spencer D, Higgins TJV (1980) The biosynthesis of legumin in maturing pea seeds. Biochem Int 1:501–509
Spencer D, Higgins TJV, Button SC, Davey RA (1980) Pulse-labelling studies on protein synthesis in developing pea seeds and evidence of a precursor form of legumin small subunit. Plant Physiol 66:510–515
Thomson JA, Schroeder HE, Dudman WF (1978) Cotyledonary storage proteins inPisum sativum. I. Molecular heterogeneity. Aust J Plant Physiol 5:263–279
Thomson JA, Schroeder HE (1978) Cotyledonary storage proteins inPisum sativum. II. Hereditary variation in components of the legumin and vicilin fractions. Aust J Plant Physiol 5:281–294
Thomson JA, Millerd A, Schroeder HE (1979) Genotype-dependent patterns of accumulation of seed storage proteins inPisum. In: Seed proteins improvement in cereal and grain legumes, vol 1. STI/PUB/496. Vienna: IAEA, pp. 231–240
Thomson JA, Schroeder HE, Tassie AM (1980) Cotyledonary storage proteins inPisum sativum. V. Further studies on molecular heterogeneity in the vicilin series of holoproteins. Aust J Plant Physiol 7:271–282
Weeke B (1973) Rocket immunoelectrophoresis. Scand J Immunol [Suppl 1] 2:37–46
Wright DJ, Boulter D (1980) Differential scanning calorimetric study of meals and constituents of some food grain legumes. J Sci Food Agric 31:1231–1241
Wright DJ, Leach IB, Wilding P (1977) Differential scanning calorimetric studies of muscle and its constituent proteins. J Sci Food Agric 28:557–564
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Casey, R., Sharman, J.E., Wright, D.J. et al. Quantitative variability inPisum seed globulins: its assessment and significance. Plant Food Hum Nutr 31, 333–346 (1982). https://doi.org/10.1007/BF01094045
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01094045