Abstract
Aflatoxin determinations can be approached many ways. Peanuts and corn are more often contaminated with aflatoxins B1 and B2 than with aflatoxins B1, B2, G1, and G2. Some countries are only interested in B1 content and others are interested in the total aflatoxin content. It is essential to safely handle all experimental materials associated with aflatoxin analyses or the aflatoxigenic fungi. Visual screening of suspect peanut lots, based on the presence of conidial heads of theAspergillus flavus group, and screening corn for the presence of bright greenish yellow fluorescence (BGYF) are not chemical tests and such screening techniques may allow aflatoxin contaminated lots into commerce. Minicolumn screening procedures should always be used in conjunction with a quantitative method. Several thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) methods are suitable for quantitation and are in general use. Immunochemical methods such as the ELISA or affinity column methods are being rapidly developed. The chemical and immunochemical methods can be reliable if care is taken, using suitable controls and personnel that are welltrained. All analytical laboratories should stress safety and include suitable analytical validation procedures.
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References
Alexander RJ, Baur MC (1977) Note on a two-dimensional TLC procedure for determining aflatoxins in corn. Cereal Chem 54:699–704
Association of Official Analytical Chemists (1984) Williams S (ed.), Official methods of analysis (14th ed) Assoc Offic Anal Chem Inc, 1111 N 19th St, Suite 210 Arlington, VA 22209
Bagley EB (1979) Decontamination of corn containing aflatoxin by treatment with ammonia. J Amer Oil Chem Soc 56:808–811
Cauderay P (1979) Rapid chemical confirmation method for aflatoxin B1 and G1 by direct acetylation on a thin layer plate before chromatography. J Assoc Offic Anal Chem 62:197
Chang-Yen I, Stoute VA, Felmine JB (1984) Effect of solvent composition on aflatoxin fluorescence. J Assoc Offic Anal Chem 67:306–308
Chu FS, Ueno I (1977) Production of antibody against aflatoxin B1. Appl Environ Microbiol 33:1125–1128
Cohen H, Lapointe M (1981) High pressure liquid chromatographic determination and fluorescence detection of aflatoxins in corn and dairy feeds. J Assoc Offic Anal Chem 64:1372–1376
Dantzman J, Stoloff L (1972) Screening method for aflatoxin in corn and various corn products. J Assoc Offic Anal Chem 55:139–141
Davis ND, Dickens JW, Freie RL, Hamilton PB, Shotwell OL, Wyllie TD (1980) Protocols for surveys, sampling, post-collection handling, and analysis of grain samples involved in mycotoxin problems. J Assoc Offic Anal Chem 63:95–102
Davis ND, Diener UL (1980) Test for the high pressure liquid chromatographic determination of aflatoxin B1. J Assoc Offic Anal Chem 63:107–109
Davis ND, Guy ML, Diener UL (1981) Improved fluorometriciodine method for determination of aflatoxin in corn. J Assoc Offic Anal Chem 64:1074–1076
DeVries JW, Chang HL (1982) Comparison of rapid high pressure liquid chromatographic and CB methods for determination of aflatoxins in corn and peanuts. J Assoc Offic Anal Chem 65:206–209
Dickens JW, Whitaker TB (1986) Sampling and sample preparation methods for mycotoxin analysis. In: Cole, RJ (ed) Modern methods in the analysis and structural elucidation of mycotoxins. Academic Press, Orlando, FL, pp 29–51
Diebold GJ, Karny N, Zare RN, Seitz LM (1979) Laser fluorometric determination of aflatoxin B1 in corn. J Assoc Offic Anal Chem 62:564–569
Ehrlich KC, Lee LS (1984) Mycotoxins in grain dust: Method for analysis of aflatoxins, ochratoxin A, zearalenone, vomitoxin, and secalonic acid. J Assoc Offic Anal Chem 67:963–967
El-NaKib O, Pestka JJ, Chu FS (1981) Determination of aflatoxin B1 in corn, wheat, and peanut butter by enzyme-linked immunosorbent assay and solid phase radioimmunoassay. J Assoc Offic Anal Chem 64:1077–1082
Fennell DI, Bothast RJ, Lillehoj EB, Peterson RE (1973) Bright greenish-yellow fluorescence and associated fungi in white corn naturally contaminated with aflatoxin. Cereal Chem 50:404–414
Friesen MD, Walker EA, Castegnaro M (1980) International mycotoxin check sample program Part I: Report on the performance of participating laboratories. J Assoc Offic Anal Chem 63:1057–1066
Garner, RC (1975) Aflatoxin separation by high-pressure liquid chromatography. J Chromatography 103:186–188
Groopman JD, Trudel LJ, Donahue PR, Marshak-Rothstein A, Wogan GN (1984) High affinity monoclonal antibodies for aflatoxins and their applications to solid-phase immunoassays. Proc Nat Acad Sci USA, 81:7728–7731
Hill RA, Wilson DM, Burg WR, Shotwell OL (1984) Viable fungi in corn dust. Appl Environ Microbiol 47:84–87
Hill RA, Wilson DM, McMillan WW, Widstrom NW, Cole RJ, Sanders TH, Blankenship PD (1985) Ecology of theAspergillus flavus group and aflatoxin formation in corn and peanut In: Lacey J (ed.) Trichothecenes and other mycotoxins. John Wiley and Sons, London pp 79–95
Holaday CE (1981) Minicolumn chromatography: State of the art, J Am Oil Chem Soc 58:931A-934A
Hutchins JE, Hagler WM (1983) Rapid liquid chromatographic determination of aflatoxins in heavily contaminated corn. J Assoc Offic Anal Chem 66:1458–1465
Josefsson BGE, Moller TE (1977) Screening method for the detection of aflatoxins, ochratoxin, patulin. sterigmatocystin, and zearalenone in cereals. J Assoc Offic Anal Chem 60:1369–1371
Kamimura H, Nishijima M, Yasuda K, Ushiyama H, Tabata S, Matsumoto S, Nishima T (1985) Simple, rapid cleanup method for analysis of aflatoxins and comparisons with various methods. J Assoc Offic Anal Chem 68:458–461
Langone JJ, Van Vunakis H (1976) Aflatoxin B1: Specific antibodies and their use in radioimmunoassay. J Nat'1 Cancer Inst 56:591–595
Lawellin DW, Grant DW, Joyce BK (1977) Enzyme-linked immunosorbent analysis for aflatoxin B1. Appl Environ Microbiol 34:94–96
Lee LS, Catalano EA (1981) Pons scaled-down clean-up column adapted for use in solvent-saving modification of the CB method for aflatoxin. J Am Oil Chem Soc 58:949A-951A
Madhyastha MS, Bhat RV (1984) Application of TLC chemical confirmatory tests to minicolumn chromatography of aflatoxins. J Amer Oil Chem Soc 61:907–908
McKinney JD, Cavanagh GC (1977) The American Oil Chemists' Society's Smalley mycotoxin check sample program: An evaluation. Ann Nutr Alim 31:519–529
Nesheim S (1971) Fading of aflatoxin spots on TLC plates during fluorescence densitometry. J Assoc Offic Anal Chem 54:1444–1445
-(1979) Method of aflatoxin analysis. In: Trace organic analysis: A new frontier in analytical chemistry. Proc 9th materials research symposium. Nat'1 Bureau of Std Spec Publ 519, pp 355–372
Nesheim S, Brumley WC (1981) Confirmation of identity of aflatoxins. J Am Oil Chem Soc 58:945A-948A
Panalaks T, Scott PM (1977) Sensitive silica gel-packed flow cell for fluorometric detection of aflatoxins by high pressure liquid chromatography. J Assoc Offic Anal Chem 60:583–589
Pestka JJ, Gaur PK, Chu FS (1980) Quantitation of aflatoxin B1 and B1 antibody by an enzyme-linked immunosorbent microassay. Appl Environ Microbiol 40:1027–1031
Pons WA Jr (1979) High pressure liquid chromatographic determination of aflatoxins in corn. J Assoc Offic Anal Chem 62:586–594
Przybylski W (1975) Formation of aflatoxin derivatives on thin layer chromatographic plates. J Assoc Offic Anal Chem 58:163–164
Rodricks JV (1973) Criteria for mycotoxin standards. J Assoc Offic Anal Chem 56:1290–1291
Romer TR, Ghouri N, Boling TM (1979) Minicolumn screening methods for detecting aflatoxin: State of the art. J Am Oil Chem Soc 56:795–797
Schuller PL, Horowitz W, Stoloff L (1976) A review of sampling plans and collaboratively studied methods of analysis for aflatoxins. J Assoc Offic Anal Chem 59:1315–1343
Seitz LM (1975) Comparison of methods for aflatoxin analysis by high pressure liquid chromatography. J Chromatography 104:81–89
Seitz LM, Mohr HE (1976) Simple method for simultaneous detection of aflatoxin and zearalenone in corn. J Assoc Offic Anal Chem 59:106–109
Shannon GM, Shotwell OL (1975) A quantitative method for determination of aflatoxin B1 in roasted corn. J Assoc Offic Anal Chem 58:743–745
—(1979) Minicolumn detection for aflatoxin in yellow corn: Collaborative study. J Assoc Offic Anal Chem 62:1070–1075
Shepard MJ, Gilbert J (1984) An investigation of HPLC postcolumn iodination conditions for the enhancement of aflatoxin B1 fluorescence. Food Addit Contamin 1:325–335
Shotwell OL (1983) Aflatoxin detection and determination in corn. In: Diener UL, Asquith RL, Dickens JW (eds) Aflatoxin andAspergillus flavus in corn. South Coop Series Bull 279, Auburn University, Auburn, AL 36849, pp 38–45
Shotwell OL, Burg WR, Diller T (1981) Thin layer chromatographic determination of aflatoxin in corn dust. J Assoc Offic Anal Chem 64:1060–1063
Shotwell OL, Goulden ML (1977) Aflatoxin: Comparison of analyses of corn by various methods. J Assoc Offic Anal Chem 60:83–88
Shotwell OL, Hesseltine CW (1980) Use of bright greenish yellow fluorescence as a presumptive test for aflatoxin in com. Cereal Chem 58(2): 124–127
Spilman JR Jr (1985) Modification of the rapid screening method for aflatoxin in corn for quantitative use. J Assoc Offic Anal Chem 68:453–458
Stoloff L, Castegnaro M, Scott P, O'Neill IK, Bartsch H, (eds) (1982) Environmental carcinogens. Selected methods of analysis, some mycotoxins, (IARC Scientific Publications No. 44). International Agency for Research on Cancer 150 cours Albert Thomas, 69372 Lyon Cedex 2, France. Vol 5, 455 pp
Stubblefield RD, Shotwell OL (1977) Reverse phase analytical and preparative high pressure liquid chromatography of aflatoxins. J Assoc Offic Anal Chem 60:784–790
Sun PS, Chu FS (1978) A simple solid-phase radioimmunoassay for aflatoxin B1. J Food Safety 1:67–75
Tarter EJ, Hanchay JP, Scott PM (1984) Improved liquid chromatographic method for determination of aflatoxins in peanut butter and other commodities. J Assoc Offic Anal Chem 67:597–600
Thean JE, Lorenz DR, Wilson DM, Rodgers K, Gueldner RC (1980) Extraction, cleanup, and quantitative determination of aflatoxins in corn. J Assoc Offic Anal Chem 63:631–633
Thomas F, Eppley RM, Trucksess MW (1975) Rapid screening method for aflatoxins and zearalenone in corn. J Assoc Offic Anal Chem 58:114–116
Trucksess MW, Brumley WC, Nesheim S (1984) Rapid quantitation and confirmation of aflatoxins in corn and peanut butter, using a disposable silica gel column, thin layer chromatography, and gas chromatography/mass spectrometry. J Assoc Offic Anal Chem 67:973–975
Velasco J (1981) Replacement of benzene as a solvent for aflatoxin standards. J Am Oil Chem Soc 58:938A-939A
Whitaker TB, Dickens JW (1983) Evaluation of a testing program for aflatoxin in corn. J Assoc Offic Anal Chem 1055–1058
Widstrom NW, Wilson DM, McMillian WW (1981) Aflatoxin contamination of preharvest corn as influenced by timing and method of inoculation. Appl Environ Microbiol 42:249–251
—(1982) Evaluation of sampling methods for detecting aflatoxin contamination in small test plots of corn inoculated withAspergillus flavus. J Environ Qual 11:655–657
Wilson DM (1987) Detection and determination of aflatoxins in corn. In: Zuber MS, Lillehoj EB, Renfro BL, (eds) Aflatoxin in corn: A proceedings of the workshop CIMMYT, Mexico, D.E, pp 100–109
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Wilson, D.M. Analytical methods for aflatoxins in corn and peanuts. Arch. Environ. Contam. Toxicol. 18, 308–314 (1989). https://doi.org/10.1007/BF01062353
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DOI: https://doi.org/10.1007/BF01062353