Abstract
The influence of sample preparation on measured concentrations of eight elements in the edible tissues of two black basses (Centrarchidae), two catfishes (Ictaluridae), and the black redhorse,Moxostoma duquesnei (Catostomidae) from two rivers in southeastern Missouri contaminated by mining and related activities was investigated. Concentrations of Pb, Cd, Cu, Zn, Fe, Mn, Ba, and Ca were measured in two skinless, boneless samples of axial muscle from individual fish prepared in a clean room. One sample (normally-processed) was removed from each fish with a knife in a manner typically used by investigators to process fish for elemental analysis and presumedly representative of methods employed by anglers when preparing fish for home consumption. A second sample (clean-processed) was then prepared from each normally-processed sample by cutting away all surface material with acid-cleaned instruments under ultraclean conditions. The samples were analyzed as a single group by atomic absorption spectrophotometry. Of the elements studied, only Pb regularly exceeded current guidelines for elemental contaminants in foods. Concentrations were high in black redhorse from contaminated sites, regardless of preparation method; for the other fishes, whether or not Pb guidelines were exceeded depended on preparation technique. Except for Mn and Ca, concentrations of all elements measured were significantly lower in cleanthan in normally-processed tissue samples. Absolute differences in measured concentrations between clean- and normally-processed samples were most evident for Pb and Ba in bass and catfish and for Cd and Zn in redhorse. Regardless of preparation method, concentrations of Pb, Ca, Mn, and Ba in individual fish were closely correlated; samples that were high or low in one of these four elements were correspondingly high or low in the other three. In contrast, correlations between Zn, Fe, and Cd occurred only in normallyprocessed samples, suggesting that these correlations resulted from high concentrations on the surfaces of some samples. Concentrations of Pb and Ba in edible tissues of fish from contaminated sites were highly correlated with Ca content, which was probably determined largely by the amount of tissue other than muscle in the sample because fish muscle contains relatively little Ca. Accordingly, variation within a group of similar samples can be reduced by normalizing Pb and Ba concentrations to a standard Ca concentration. When sample size (N) is large, this can be accomplished statistically by analysis of covariance; whenN is small, molar ratios of [Pb]/[Ca] and [Ba]/[Ca] can be computed. Without such adjustments, unrealistically large Ns are required to yield statistically reliable estimates of Pb concentrations in edible tissues. Investigators should acknowledge that reported concentrations of certain elements are only estimates, and that regardless of the care exercised during the collection, preparation, and analysis of samples, results should be interpreted with the awareness that contamination from external sources may have occurred.
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References
Barry PSI (1978) Distribution and storage of lead in human tissues. In: Nriagu JR (ed) The biogeochemistry of lead in the environment. Part B. Biological effects. Elsevier/North-Holland, Amsterdam, The Netherlands, p 97
Berman E (1976) The challenge of getting the lead out. In: LaFleur PD (ed) Accuracy in trace analysis: Sampling, sample handling, analysis-Volume II. Proceedings of the 7th IMR Symposium, National Bureau of Standards, Special Publication 422, Washington, DC, p 715
Bowman ML (1970) Life history of the black redhorse,Moxostoma duquesnei (Lesueur), in Missouri. Trans Am Fish Soc 99:546–559
Brooks RR, Rumsey MG (1974) Heavy metals in some New Zealand commercial sea fishes. New Zealand J Mar Freshwater Res 8:155–166
Bryce-Smith D, Stephens R (1983) Sources and effects of environmental lead. In: Rose J (ed) Trace elements in health. A review of current issues. Butterworths, London, England, p 83
Chow TJ, Patterson CC, Settle D (1974) The occurrence of lead in tuna. Nature (London) 251:159–161
Coombs TL, Fletcher TC, White A (1972) Interaction of metal ions with mucus from the plaice (Pleuronectes platessa L.). Biochem J 128:128–129
Czarneski JM (1985) Accumulation of lead in fish from Missouri streams impacted by lead mining. Bull Environ Contam Toxicol 34:736–745
Eddy F, Fraser JE (1982) Sialic acid and mucus production in rainbow trout (Salmo gairdneri Richardson) in respone to zinc in seawater. Comp Biochem Physiol 73C:357–359
Eisler R (1984) Trace metal changes associated with age of marine vertebrates. Biol Trace Element Res 6:165–180
Fletcher TC, Grant PT (1968) Glycoproteins in the external mucous secretions of the plaice,Pleuronectes platessa, and other fishes. Biochem J 106:12
Flik G, Van Rijs JH, Wendelaar Bonga SE (1984) Evidence for the presence of calmodulin in fish mucus. Europ J Biochem 138:651–654
Freund RJ, Littel RC (1981) SAS for linear models. A guide to the ANOVA and GLM procedures. SAS Institute, Cary, NC, 231 pp
Gale N, Bolter E, Wixson B (1976) Investigation of Clearwater Lake as a potential sink for heavy metals from lead mining in Southeast Missouri. In: Hemphill D (ed) Proceedings of the 10th Annual Conference on Trace Substances in Environmental Health, University of Missouri-Columbia, p 187
Gale N, Haride MC, Jennett JC, Aloti A (1973) Transport of trace pollutants in lead mining waters. In: Hemphill D (ed) Proceedings of the 6th Annual Conference on Trace Substances in Environmental Health, University of Missouri-Columbia, p 95
Gale N, Wixson BC, McMenus MW (1982) Lead concentrations in edible fillets collected from Missouri's Old Lead Belt. In: Hemphill D (ed) Proceedings of the 14th Annual Conference on Trace Substances in Environmental Health, University of Missouri-Columbia, p 12
Giesy JP Jr, Wiener JG (1977) Frequency distributions of trace metal concentrations in five freshwater fishes. Trans Am Fish Soc 106:393–423
Hall RA, Zook EG, Meaburn GM (1978) National Marine Fisheries Service survey of trace elements in the fishery resource. US Department of Commerce, National Oceanic and Atmospheric Administration, Rockville, MD, SSRF-721, 313 pp
Hardie MG, Jennet JC, Bolter E, Wixson B, Gale N (1974) Water resources problems and solutions associated with the New Lead Belt of S.E. Missouri. In: Hadley RF, Snow DT (eds) Water resources problems related to mining. American Water Resources Association, Minneapolis, MN, p 109
Harwood J (1984) Effects of cover materials on leaching of toxic metals from lead mine tailings. Unpubl. PhD Thesis, University of Missouri-Columbia, 138 pp
Heit M (1979) Variability of the concentrations of seventeen trace elements in the muscle and liver of a single striped bass,Morone saxatilis. Bull Environ Contam Toxicol 23:1–5
Hocutt CH, Stauffer JR Jr, Mills PA (1978) Influence of a barite tailings pond rupture on the fishes of Big River, Missouri. In: Samuel ED, Stauffer JR, Hocutt CH, Mason WT Jr (eds) Surface mining and fish/wildlife needs in the Eastern United States. US Fish and Wildlife Service, FWS/OBS-78/81. Washington, DC, p 177
Hodson PV, Blunt BR, Spry DJ, Austen K (1977) Evaluation of erythrocyte 8-amino levulinic acid dehydratase (ALA-D) activity on a short-term indicator in fish of a harmful exposure to lead. J Fish Res Board Can 34:501–508
Huitema BE (1980) The analysis of covariance and alternatives. J Wiley, New York, 445 pp
Kaiser ML, Koirtyohann SR, Hinderberger EJ, Taylor HE (1981) Reduction of matrix interferences in furnace atomic absorption with the L'vov platform. Spectrochim Acta 36B:773–783
Kirschner LB (1977) External charged layer and Na+ regulation. In: Jorgensen CB, Skadhagne E (eds) Osmotic and volume regulation (Alfred Benzon Symposium XI). Academic Press, New York, p 310
Kramer R (1976) Effects of a century old Missouri lead mining operation upon the water quality, sediments and biota of Flat River Creek. Unpublished MSc Thesis, University of Missouri Rolla, 137 pp
Kussmaul K, Anderson RL (1967) Estimation of variance components in two-stage nested designs with composite samples. Technometrics 9:373–389
Lemly AD (1985) Toxicology of selenium in a freshwater reservoir: Implications for environmental hazard evaluation and safety. Ecotoxicol Environ Safety 10:314–338
Lock RAC, Van Overbeck AP (1981) Effects of mercuric chloride on mucus secretion in rainbow trout (Salmo gairdneri Richardson). Comp Biochem Physiol 69C:67–73
Mahaffey KR (1978) Environmental exposure to lead. In: Nriagu JO (ed) The biogeochemistry of lead in the environment. Part B. Biological effects. Elsevier/North-Holland, Amsterdam, The Netherlands, p 2
Moore JW, Ramamoorthy S (1983) Heavy metals in natural waters. Applied monitoring and impact analysis. Springer-Verlag, New York, 268 pp
Niethammer KR, Atkinson RD, Baskett TS, Sampson FB (1985) Metals in riparian wildlife of the lead-mining district of Southeastern Missouri. Arch Environ Contam Toxicol 14:213–223
Novak JT, Hasselwander GB (1980) Control of mine tailing discharge to Big River. Missouri Department of Natural Resources Report, Jefferson City, 75 pp
O'Shea TA, Mancy KH (1978) The effect of pH and hardness metal ions on the competitive interaction between trace metal ions and inorganic and organic complexing agents found in natural waters. Water Res 12:703–711
Part P, Lock RAC (1983) Diffusion of calcium, cadmium and mercury in a mucous solution from a rainbow trout. Comp Biochem Physiol 76C:259–263
Patterson CC (1980) An alternative perspective-lead pollution in the human environment: Origin, extent, and significance. In: Lead in the human environment. National Research Council, Committee on Lead in the Environment, Washington, DC, p 265
Patterson CC, Settle DM (1976) The reduction of orders of magnitude errors in lead analyses of biological materials and of natural waters by evaluating and controlling the extent and sources of industrial lead contamination introduced during sample collecting, handling, and analysis. In: LaFleur PD (ed) Accuracy in trace analysis: sampling, sample handling, analysis—Volume I. Proceedings of the 7th IMR Symposium. National Bureau of Standards, Special Publication 422, Washington, DC, p 321
— (1977) Comparative distributions of alkalies, alkaline earths and lead among major tissues of the tunaThunus alalunga. Mar Biol 39:289–295
Pflieger WL (1975) The fishes of Missouri. Missouri Department of Conservation, Columbia, 343 pp
Phillips DJH (1977) The use of biological indicator organisms to monitor trace metal polution in marine and estuarine environments—a review. Environ Pollut 13:281–317
Probst WE, Rabeni CF, Covington WG, Marteney RE (1984) Resource use by stream-dwelling rock bass and smallmouth bass. Trans Am Fish Soc 113:283–294
Purkett CA Jr (1958) Growth of fishes in the Salt River, Missouri. Trans Am Fish Soc 87:116–131
Richkus WA (1984) Considerations in the selection of commercial analytical laboratories. Fisheries (Bethesda) 9:12–16
Ricker WE (1973) Linear regressions in fishery research. J Fish Res Board Can 30:409–434
SAS Institute (1982a) SAS user's guide: Basics, 1982 edition. Cary, NC 923 pp
- (1982b) SAS user's guide: Statistics, 1982 edition. Cary, NC, 584 pp
Schmitt CJ, Dwyer FJ, Finger SE (1984) Bioavailability of Pb and Zn from mine tailings as indicated by erythrocyte 8-aminolevulinic acid dehydratase (ALA-D) activity in suckers (Pisces:Catostomidae). Can J Fish Aquat Sci 41:1030–1040
Schmitt CJ, Finger SE (1982) The dynamics of metals from past and present mining activities in the Big and Black River watersheds, southeastern Missouri. Completion Report, US Army Corps of Engineers, St. Louis District, Project No. DACW43-80-A-0109, 152 pp
Settle DM, Patterson CC (1980) Lead in albacore: Guide to lead pollution in Americans. Science 207:1167–1176
Shirk M (1980) Warnings of eating some fish from Big River. St. Louis Post-Dispatch 102:6A
Sidwell YD, Loomis AL, Loomis KJ, Foncannon PR, Buzzel DH (1978) Composition of the edible portion of raw (fresh or frozen) crustaceans, finfish, and molluscs. III. Microelements. Mar Fish Rev 40, MFR Paper 1324, 20 pp
Sokal RR, Rohlf FJ (1969) Biometry. The principals and procedures of statistics in biological research. WH Freeman, San Francisco, 776 pp
Steel RGD, Torrie JH (1980) Principles and procedures of statistics. A biometrical approach. Second Edition. McGraw-Hill, New York, 633 pp
U.S. Geological Survey (1967) Mineral and water resources of Missouri. Volume XLIII. U.S. Government Printing Office, Washington, DC, 399 pp
Van Oosten J (1957) The skin and scales. In: Brown ME (ed) The physiology of fishes, Volume I. Metabolism. Academic Press, New York, p 207
Varanasi U, Markey D (1978) Uptake and release of lead and cadmium in skin and mucus of coho salmon (Oncorhynchus kisutch). Comp Biochem Physiol 60C:187–191
Varanasi U, Robisch PA, Malins DA (1975) Structural alterations in fish epidermal mucus produced by water-borne lead and mercury. Nature (London) 258:431–432
Whelan G (1983) The distribution of lead and cadmium within a lotic benthic community. Unpublished MSc Thesis, University of Missouri-Columbia. 157 pp
Whitley JR (1979) Big River-big problem. Mo Conserv 40:20–22
Wiener JG (1982) Method for detecting trace-element contamination of fish samples from handling. Environ Sci Technol 16:90–93
Wiener JG, Giesy JP Jr (1979) Concentrations of Cd, Cu, Mn, Pb, and Zn in fishes in a highly organic softwater pond. JFish Res Board Can 36:270–279
Wixson B, Bolter E (1972) Evaluation of stream pollution and trace substances in the New Lead Belt of Missouri. In: Hemphill D (ed) Proceedings of the 5th Annual Conference on Trace Substances in Environmental Health, University of Missouri-Columbia, p 143
Wold JK, Selset R (1977) Glycoproteins in the skin mucus of the char (Salmo alpinus L.). Comp Biochem Physiol 56B:215–218
— (1978) Glycoproteins in the skin mucus of the char (Salmo alpinus L.)—II. Production of mucous after death offish. Comp Biochem Physiol 616:271–273
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Schmitt, C.J., Finger, S.E. The effects of sample preparation on measured concentrations of eight elements in edible tissues of fish from streams contaminated by lead mining. Arch. Environ. Contam. Toxicol. 16, 185–207 (1987). https://doi.org/10.1007/BF01055800
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DOI: https://doi.org/10.1007/BF01055800