Abstract
Twenty healthy adult humans had serum samples drawn on four occasions within a 24-hr period: after a 12 hr overnight fast, 4–5 hr after a high fat breakfast, at midafternoon, and the next morning after another 12 hr fast. Nonfasting samples had 22% to 29% higher mean concentrations (p < 0.05) than did fasting samples for polychlorinated biphenyls (PCBs, 4.81 vs 3.74 ng/g serum wt), hexachlorobenzene (HCB, 0.163 vs 0.134 ng/g serum wt), andp,p′-dichlorodiphenyldichloroethylene (p,p′-DDE, 6.74 vs 5.37 ng/g serum wt) measured by electron capture gas liquid chromatography. Total serum lipids were estimated from measurements of total cholesterol, free cholesterol, triglycerides, and phospholipids and were 20% higher in nonfasting samples than in fasting samples (7.05 g/L vs 5.86 g/L). When PCBs, HCB, andp,p′-DDE concentrations were corrected by total serum lipids, results from fasting and nonfasting samples were not statistically different. Because of the differences in these chlorinated hydrocarbon concentrations observed with different sample collection regimens, meaningful comparison of analytical results requires standardizing collection procedures or correcting by total serum lipid levels.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Birnbaum LS (1985) The role of structure in the disposition of halogenated aromatic xenobiotics. Environ Health Perspect 61:11–20
Brown JF, Lawton RW (1984) Polychlorinated biphenyl (PCB) partitioning between adipose tissue and serum. Bull Environ Contain Toxicol 33:277–280
Burse VW, Needham LL, Lapeza CR, Korver MP, Liddle JA, Bayse DD (1983) Evaluation of a potential analytical approach for determination of polychlorinated biphenyls in serum: Interlaboratory study. J Assoc Off Anal Chem 66:956–968
Cheek CS, Wease DF (1969) A summation technic for serum total lipids:comparison of methods. Clin Chem 15:102–107
Cohn JS, McNamara JR, Cohn SD, Ordovas JM, Schaefer EJ (1988) Postprandial plasma lipoprotein changes in human subjects of different ages. J Lipid Res 29:469–479
Cooper GR, Duncan PH, Hazelhurst JS, Miller DT, Bayse DD (1982) Cholesterol. Enzymic Method. In:Faulkner WR, Meites S (eds) Selected Methods for the Small Clinical Chemistry Laboratory, Vol 9. American Association for Clinical Chemistry, Washington, DC, pp 165–174
Kreiss K, Zack MM, Kimbrough RD, Needham LL, Smrek AL, Jones BT (1981a) Association of blood pressure and polychlorinated biphenyl levels. JAMA 245:2505–2509
— (1981b) Cross-sectional study of a community with exceptional exposure to DDT. JAMA 245:1926–1930
Lutz RJ, Dedrick RL, Matthews HB, Eling TE, Anderson MW (1977) A preliminary pharmacokinetic model for several chlorinated biphenyls in the rat. Drug Metab Dispos 5:386–396
Maliwal BP, Guthrie FE (1981) Interaction of insecticides with human plasma lipoproteins. Chem Biol Interact 35:177–188
— (1982)In vitro uptake and transfer of chlorinated hydrocarbons among human lipoproteins. J Lipid Res 23:474–479
Matthews HB (1980) Disposition of persistent halogenated hydrocarbons in higher animals. In:Khan MAQ, Stanton RH (eds) Toxicology of halogenated hydrocarbons:Health and ecological effects. Pergamon, New York, p 289–297
Matthews HB, Anderson MW (1975) Effect of chlorination on the distribution and excretion of polychlorinated biphenyls. Drag Metab Dispos 3:371–380
Matthews HB, Dedrick RL (1984) Pharmacokinetics of PCBs. Ann Rev Pharmacol Toxicol 24:85–103
Matthews HB, Surles JR, Carver JG, Anderson MW (1984) Halogenated biphenyl transport by blood components. Fund Appl Toxicol 4:420–428
Morgan DP, Roan CC (1970) Chlorinated hydrocarbon pesticide residue in human tissues. Arch Environ Health 20:452–457
Murphy R, Harvey C (1985) Residues and metabolites of selected persistent halogenated hydrocarbons in blood specimens from a general population survey. Environ Health Perspect 60:115–120
National Heart, Lung, and Blood Institute (1980) The Lipid Research Clinics Population Studies Data Book, Vol 1, The Prevalence Study. NIH Publication 80-1527
Patterson DG, Needham LL, Pirkle JL, Roberts DW, Bagby J, Garrett WA, Andrews JS, Falk H, Bernert JT, Sampson EJ, Houk VN (1988). Correlation between serum and adipose tissue levels of 2,3,7,8-tetrachlorodibenzo-p-dioxin in 50 persons from Missouri. Arch Environ Contam Toxicol 17:139–143
Safe S (1984) Polychlorinated biphenyls (PCBs) and polybrominated biphenyls (PBBs):Biochemistry, toxicology, and mechanism of action. CRC Crit Rev Toxicol 13:319–365
SAS Institute, Inc (1985) SAS User's Guide:Statistics, Version 5. SAS Institute, Inc., Cary, NC
Sokal RR, Rohlf FJ (1969) Biometry. WH Freeman, San Francisco
Takayama M, Itoh S, Nagasaki T, Tanamizu I (1977) A new enzymatic method for determination of serum choline-containing phospholipids. Clin Chim Acta 79:93–98
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Phillips, D.L., Pirkle, J.L., Burse, V.W. et al. Chlorinated hydrocarbon levels in human serum: Effects of fasting and feeding. Arch. Environ. Contam. Toxicol. 18, 495–500 (1989). https://doi.org/10.1007/BF01055015
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01055015