Abstract
The occurrence of differences in acute-phase response, with respect to concentration and glycosylation of α1-acid glycoprotein (AGP) was studied in the sera of patients surviving or not from septic shock. Crossed affino-immunoelectrophoresis was used with concanavalin A andAleuria aurantia lectin for the detection of the degree of branching and fucosylation, respectively, and the monoclonal CSLEX-1 for the detection of sialyl Lewisx (SLeX) groups on AGP. Septic shock apparently induced an acute-phase response as indicated by the increased serum levels and changed glycosylation of AGP. In the survivor group a transient increase in diantennary glycan content was accompanied by a gradually increasing fucosylation and SLeX expression, comparable to those observed in the early phase of an acute-inflammatory response. Remarkably, in the non-survivor group a modest increase in diantennary glycan content was accompanied by a strong elevation of the fucosylation of AGP and the expression of SLeX groups on AGP, typical for the late phase of an acute-phase response. Our results suggest that these changes in glycosylation of AGP can have a prognostic value for the outcome of septic shock.
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Abbreviations
- AAL:
-
Aleuria aurantia lectin
- AGP:
-
α1-acid glycoprotein
- CAIE:
-
crossed affinoimmunoelectrophoresis
- Con A:
-
Concanavalin A
- HSPC:
-
human serum protein calibrator
- IL-1:
-
interleukin 1
- IL-6:
-
interleukin 6
- LIF:
-
leukaemia inhibitory factor
- LPS:
-
lipopolysaccharide
- SLeX:
-
sialyl Lewisx
- TNF:
-
tumour necrosis factor
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Brinkman-Van Der Linden, E.C.M., Van Ommen, E.C.R. & Van Dijk, W. Glycosylation ofα 1 glycoprotein in septic shock: Changes in degree of branching and in expression of sialyl Lewisx groups. Glycoconjugate J 13, 27–31 (1996). https://doi.org/10.1007/BF01049676
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DOI: https://doi.org/10.1007/BF01049676