Abstract
The use of casein and non-fat dry milk for the recovery of enteroviruses from seawater and tapwater was investigated. Poliovirus type 1 was recovered with high efficiency (>70% overall recovery) from large volumes of seawater or tapwater, using 1% (w/v) non-fat dry milk buffered at pH 9.0 with 0.05M glycine. Good recovery of low numbers of poliovirus 1 from tapwater was also achieved. The method can also recover other enteroviruses (Poliovirus 2, 3, coxsackievirus B3 and echovirus 4), but appeared inefficient in the recovery of echovirus 1.
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Bitton, G., Feldberg, B.N. & Farrah, S.R. Concentration of enteroviruses from seawater and tapwater by organic flocculation using non-fat dry milk and casein. Water Air Soil Pollut 12, 187–195 (1979). https://doi.org/10.1007/BF01047122
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DOI: https://doi.org/10.1007/BF01047122