Abstract
The equilibrium dialysis method was applied to the determination of drug-cyclodextrin stability constants using diflunisal and 2-hydroxypropyl-β-cyclodextrin (HPBCD) as a model system. Analysis of data showed the existence of a linear Scatchard plot, which is indicative of the formation of a 1:1 dlflunisal:LIPBCD complex. The stoichiometry of the complex was verified using the appropriate mass action law equation. The complexation constant (Kc) was 3801 ± 541 M−1. The Kc obtained using the equilibrium dialysis method was comparable to that obtained using a potentiometric method (5564 ± 56 M−1).
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
M.C. Meyer and D.E. Guttman. The binding of drugs by plasma proteins.J. Pharm. Sci.,57:895–918 (1968).
K.A. Connors. Binding Constants: The Measurement of Molecular Complex Stability, John Wiley & Sons, New York, NY, 1987, p. 266.
E.E. Sideris, M.A. Koupparis and P.E. Macheras. Effect of cyclodextrins on protein binding of drugs: the diflunisal/hydroxypropyl-β-cyclodextrin model case.Pharm. Res. 11:90–95 (1994).
M. Vikmon. Rapid and simple spectrophotometric method for determination of micro-amounts of cyclodextrins. 1st Int. Sympos. Cyclodextrins, 1981, Budapest, Hungary, pp. 69-74.
G.F. Lockwood and J.G. Wagner. Plasma volume changes as a result of equilibrium dialysis.J. Pharm. Pharmacol. 35:387–399 (1983).
G. Scatchard. The attractions of proteins for small molecules and ions.Ann. N.Y. Acad. Sci.,51:660–672 (1949).
T.W. Plumbridge, L.J. Aarons and J.R. Brown. Problems associated with analysis and interpretation of small molecule/macromolecule binding data.J. Pharm. Pharmacol. 30:69–74 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ugwu, S.O., Alcala, M.J., Bhardwaj, R. et al. The application of equilibrium dialysis to the determination of drug-cyclodextrin stability constants. J Incl Phenom Macrocycl Chem 25, 173–176 (1996). https://doi.org/10.1007/BF01041563
Issue Date:
DOI: https://doi.org/10.1007/BF01041563