Summary
The temperature induced intracellular production of the fused protein SpA-βgal (protein A/β-galactosidase) withE. coli was compared in batch and fed batch culture. By introducing fed batch cultivation a final cell dry weight of 77.0 g/l was achieved, as compared to 16.4 g/l in batch cultivation. The concentration of SpA-βgal in the fed batch cultivation was very high, 19.2 g/l. This corresponded to 25% of the cell dry weight.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bech Jensen, E., and Carlsen, S. (1990).Biotechnol. Bioeng., 36, 1–11.
Betenbaugh, M.J., Beaty, C., and Dhurjati, P. (1989).Biotechnol. Bioeng., 33, 1425–1436.
Fieschko, J., and Ritch, T. (1986).Chem. Eng. Commun., 45, 229–240.
Förberg, C., Enfors, S.-O., and Häggström, L. (1983).Eur. J. Appl. Microbiol. Biotechnol., 17, 143–147.
Hellebust, H., Murby, M., Abrahmsén, L., Uhlén, M., and Enfors, S.-O. (1989).Bio/Technology, 7, 165–168.
Holme, T., Arvidson, S., Lindholm, B., and Pavlu, B. (1970).Process Biochem., 5, 62–66.
Jung, G., Denèfle, P., Becquart, J., and Mayaux, J.-F. (1988).Ann. Inst. Pasteur/Microbiol., 139, 129–146.
Kaprálek, F., Jecmen, P., Sedlácek, J., Fábry, M., and Zadrazil, S. (1991).Biotechnol. Bioeng., 37, 71–79.
Luli, G.W., and Strohl, W.R. (1990).Appl. Environ. Microbiol., 56, 1004–1011.
Majewski, R.A., and Domach, M.M. (1990).Biotechnol. Bioeng., 35, 732–738.
Nilsson, B., and Abrahmsén, L. (1990).Methods Enzym., 185, 144–161.
Reiling, H.E., Laurila, H., and Fiechter, A. (1985).J. Biotechnol., 2, 191–206.
Riesenberg, D., Menzel, K., Schulz, V., Schumann, K., Veith, G., Zuber, G., and Knorre, W.A. (1990).Appl. Microbiol. Biotechnol., 34, 77–82.
Strandberg, L., and Enfors, S.-O. (1991).Appl. Environ. Microbiol., 57, 1669–1674.
Strandberg, L., Köhler, K., and Enfors, S.-O. (1991).Process Biochem., in press.
Sugimoto, S., Kato, N., Seki, T., Yoshida, T. and Taguchi, H. (1987).J. Biotechnol., 5, 157–163.
Thompson, B.G., Kole, M., and Gerson, D.F. (1985).Biotechnol. Bioeng., 27, 818–824.
Veide, A., Smeds, A.-L., and Enfors, S.-O. (1983).Biotechnol. Bioeng., 25, 1789–1800.
Yano, T., Mori, H., Kobayashi, T., and Shimizu, S. (1980).J. Ferment. Technol., 58, 259–266.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Strandberg, L., Enfors, SO. Batch and fed batch cultivations for the temperature induced production of a recombinant protein inEscherichia coli . Biotechnol Lett 13, 609–614 (1991). https://doi.org/10.1007/BF01033419
Issue Date:
DOI: https://doi.org/10.1007/BF01033419