Abstract
The enzyme alkaline phosphatase, EC 3. 1. 3. 1, was found to be active in a supercritical carbo dioxide solvent system. A batch reaction of disodium p-nitrophenyl phosphate with a 0.1 vol. % water solution in supercritical CO2 at 100 atm and 35°C produced p-nitrophenol when catalyzed by alkaline phosphatase.
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References
Bulter, L.G., Enzyme Microb. Technol., Vol. 1, pp. 253–259, (1979).
de Fillipi, R., Chemistry and Industry, p. 390, June 19, 1982.
Gardner, D.S.J., “Industrial Scale Hops Extraction,” presented at the Soc. Chem. Ind. Food Engineering Panel Symposium, CO2 in Solvent Extraction, London, 1982.
Kurzals, H., “Caffeine Extraction,” presented at the Soc. Chem. Ind. Food Engineering Panel Symposium, CO2 in Solvent Extraction, London, 1982.
Vick Roy, J.R., and A. D. Converse, “Biomass Hydrolysis with Sulfur Dioxide and Water In the Region of the Critical Point,” presented at the 1984 A1ChE Annual Meeting, San Francisco, November, 1984.
Zaks, A., and A.M. Klibanov, Science, vol. 224. pp. 1249–1251, June 15, 1984.
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Randolph, T.W., Blanch, H.W., Prausnitz, J.M. et al. Enzymatic catalysis in a supercritical fluid. Biotechnol Lett 7, 325–328 (1985). https://doi.org/10.1007/BF01030279
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DOI: https://doi.org/10.1007/BF01030279