Summary
Reducing the size of crude cellulase from Trichoderma reesei with various proteases showed that while the β-glucosidase activity on CMC generally increased, the extent of hydrolysis of hardwood and pretreated hardwood decreased. The rank in the decrease in hydrolytic activity is the same as the rank in the decrease in enzyme adsorption on the hardwood or pretreated hardwood.
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Chen, H.C., Grethlein, H.E. Effect of cellulase size reduction on activity and accessibility. Biotechnol Lett 10, 913–918 (1988). https://doi.org/10.1007/BF01027005
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DOI: https://doi.org/10.1007/BF01027005