Summary
A continuous culture of insect cells (Spodoptera frugiperda) was used for continuous production of baculovirus (nuclear polyhedrosis virus fromAutographa californica). The system consisted of a cascade of two continuous stirred tank reactors (CSTRs). In CSTR I the insect cells were grown in suspension. This suspension was fed continuously to CSTR II where the virus infection occurred. For a period of about 25 days the average volumetric productivity was about 107 polyhedra (virus particles occluded in protein capsules) and 108 infectious NOVs (non-occluded virus particles) per cm3 effluent. This is equivalent to 25 polyhedra and 250 NOVs per infected cell, respectively. In one case, the percentage of infected cells was 65%, which is close to the theoretical value of 68%. After a run-time of 32 days a decrease of process productivity was observed, probably due to the so-called passage effect, a degeneration of the virus DNA.
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Kompier, R., Tramper, J. & Vlak, J.M. A continuous process for the production of baculovirus using insect-cell cultures. Biotechnol Lett 10, 849–854 (1988). https://doi.org/10.1007/BF01026994
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DOI: https://doi.org/10.1007/BF01026994