Abstract
The interactions of fatty acids with porcine and bovine β-lactoglobulins were measured using tryptophan fluorescence enhancement. In the case of bovine β-lactoglobulin, the apparent binding constants for most of the saturated and unsaturated fatty acids were in the range of 10−7 M at neutralpH. Bovine β-lactoglobulin displays only one high affinity binding site for palmitate with an apparent dissociation constant of 1·10−7 M. The strength of the binding was decreasing in the following way: palmitate > stearate > myristate > arachidate > laurate. Caprylic and capric acids are not bound at all. The affinity of β-lactoglobulin for palmitate decreased as thepH of the incubation medium was lowered and BLG/palmitate complex was not observed atpH's lower than 4.5. Surprisingly, chemically modified bovine β-lactoglobulin and porcine β-lactoglobulin did not bind fatty acids in the applied conditions.
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References
Cabacungan, J. C., Ahmed, A. I., and Feeney, R. E. (1982).Anal. Biochem. 124 272–278.
Cogan, U., Kopleman, M., Mokady, S., and Shinitzky, M. (1976).Eur. J. Biochem. 65 71–78.
Coke, M., Wilde, P. J., Russel, E. J. and Clark, D. C. (1990).J. Colloid Interface Sci. 138 489–504.
Dalgalarrondo, M., Dufour, E., Bertrand-Harb, C., Chobert, J. M., and Haertlé, T. (1992).Le Lait 72 35–42.
Davis, B. D., and Dubos, R. J. (1947).J. Expt. Medicine 86 215–227.
Della, N. K. D., and Kinsella, J. E. (1988).Biochem. J. 255 113–118.
Diaz de Villegas, M. C., Oria, R., Sala, F., and Calvo, M. (1987).Milchwissenschaft 42 357–358.
Dodin, G., Andrieux, M., and Al Kabbani, H. (1990).Eur. J. Biochem. 193 697–700.
Dufour, E., and Haertlé, T. (1990).J. Agric. Food Chem. 38 1691–1695.
Dufour, E., and Haertlé, T. (1991).Biochim. Biophys. Acta 1079 316–320.
Dufour, E., Marden, M. C., and Haertlé, T. (1990).FEBS Lett. 277 223–226.
Folch, J., Lees, M., and Stanley, H. L. (1957).J. Biol. Chem. 226 497–509.
Fraenkel-Conrat, H., and Olcott, H. S. (1945).J. Biol. Chem. 161 259–268.
Fugate, R. D., and Song, P. S. (1980).Biochim. Biophys. Acta 625 28–42.
Futterman, S., and Heller, J. (1972).J. Biol. Chem. 247 5168–5172.
Godovac-Zimmerman, J. (1988).Trends Biochem. Sci. 13 64–66.
Holden, H. M., Rypniewski, W. R., Law, J. H., and Rayment, I. (1987).EMBO J. 6 1565–1570.
Huber, R., Schneider, M., Epp, O., Mayr, I., Messerschmidt, A., Pflugrath, J., and Kayser, H. (1987).J. Mol. Biol. 195 423–434.
Kessler, E., and Brew, K. (1970).Biochim. Biophys. Acta 200 449–458.
Mailliart, P., and Ribadeau-Dumas, B. (1988).J. Food Sci. 53 743–745.
McKeekin, T. L., Polis, B. D., Della Monica, E. S., and Custer, J. H. (1949).J. Am. Chem. Soc. 71 3606–3609.
Mohan Reddy, I., Kella, N. K. D., and Kinsella, J. E. (1988).J. Agric. Food Chem. 36 737–747.
Monaco, H. L., Zanotti, G., Spadon, P., Bolognesi, M., Sawyer, L., and Eliopoulos, E. E. (1987).J. Mol. Biol. 197 695–706.
Morrison, W. R., and Smith, L. M. (1964).J. Lipid Res. 5 600–608.
Newcomer, M. E., Jones, T. A., Aqvist, J., Sundelin, J., Eriksson, U., Rask, L., and Peterson, P. (1984),EMBO J. 3 1451–1454.
North, A. C. T. (1989).Int. J. Biol. Macromol. 11 56–68.
O'Neill, T. E., and Kinsella, J. E. (1987).J. Agric. Food Chem. 35 770–774.
Papiz, M. Z., Sawyer, L., Eliopoulos, E. E., North, A. C. T., Findlay, J. B. C., Sivaprasadarao, R., Jones, T. A., Newcomer, M. E., and Kraulis P. J. (1986).Nature 324 383–385.
Perez, M. D., Diaz de Villegas, C., Sanchez, L., Aranda, P., Ena, J. M., and Calvo, M. (1989).J. Biochem. 106 1094–1097.
Perez, M. D., Sanchez, L., Aranda, P., Ena, J. M., Oria, R., and Calvo, M. (1992).Biochim. Biophys. Acta 1123 151–155.
Spector, A. A., and Fletcher, J. E. (1970).Lipids 5 403–411.
Takeda, K., and Moriyama, Y. (1989).J. Prot. Chem. 8 487–494.
Tanford, C., and De, P. K. (1961).J. Biol. Chem. 236 1711–1715.
Timasheff, S. N., and Townend, R. (1964).Nature 203 517–519.
Tonsgard, J. H., and Meredith, S. C. (1991).Biochem. J. 276 569–575.
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Frapin, D., Dufour, E. & Haertle, T. Probing the fatty acid binding site of β-lactoglobulins. J Protein Chem 12, 443–449 (1993). https://doi.org/10.1007/BF01025044
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DOI: https://doi.org/10.1007/BF01025044