Summary
We describe the production of L-phenylalanine from phenylpyruvate using resting cells of a genetically modified strain of Escherichia coli. Fermentations were carried out by continuously raising the feeding rate of D-glucose. We reached a biomass of 10 g/l dry weight and an aminotransferase activity of 14000 U/l The maximum phenylalanine concentration achieved was 173 mmol/l with a phenylpyruvate molar conversion yield of 95%.
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Dedicated to Prof. Dr. Heinz Harnisch on the occasion of his 60th birthday
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Then, J., Doherty, A., Neatherway, H. et al. Production of L-phenylalanine from phenylpyruvate using resting cells of Escherichia coli. Biotechnol Lett 9, 680–684 (1987). https://doi.org/10.1007/BF01024597
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DOI: https://doi.org/10.1007/BF01024597