Summary
6-methyl-5-hepten-2-one was reduced to sulcatol ((+)-6-methyl-5-hepten-2-ol) by using alcohol dehydrogenase fromThermoanaerobium brockii in a continuous process. The cofactor NADP(H) was retained by a charged UF-membrane and regenerated by oxidation of isopropanol to acetone. Use of native NADP in a charged UF-membrane reactor proved to be superior to use of PEG coupled NADP in a uncharged UF-membrane reactor.
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Röthig, T.R., Kulbe, K.D., Bückmann, F. et al. Continuous coenzyme dependent stereoselective synthesis of sulcatol by alcohol dehydrogenase. Biotechnol Lett 12, 353–356 (1990). https://doi.org/10.1007/BF01024430
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DOI: https://doi.org/10.1007/BF01024430