Summary
In order to develop a protocol for the release of intravacuolarly-stored anthocyanins from grapevine (Vitis vinifera L.) cell suspension with preserved viability, the effects of extracellular pH modifications, of solid adsorbents in the culture medium (two-phase system) and of chemical permeabilization were investigated. The solid adsorbent Amberlite IR-120 and chemical permeabilization were efficient in enabling the release of up to 70–80% of vacuolar anthocyanins. However, the release was always at the expense of a proportionate loss in cell viability. Results demonstrate the irreversible nature of anthocyanin sequestration.
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FRDC Manuscript No. 260.
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Cormier, F., Do, C.B., Moresoli, C. et al. Anthocyanin release from grape (Vitis vinifera L.) cell suspension. Biotechnol Lett 14, 1029–1034 (1992). https://doi.org/10.1007/BF01021053
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DOI: https://doi.org/10.1007/BF01021053