Summary
An α-glucosidase fromAspergillus carbonarious CCRC 30414 was employed for investigating the enzymatic synthesis of isomaltooligosaccharides from maltose. The enzyme transferred a glucose unit from the nonreducing end of maltose and other α-linked glucosyl oligosaccharides to glucose and other glucosyl oligosaccharides which function as accepting co-substrates. The transfer of a glucose unit occurs most frequently to the 6-OH position of the nonreducing end of acceptor, but transfer to 4-OH position also occurs. Treatment of 30 % (w/v) maltose with the enzyme under optimum conditions afforded more than 50% isomaltooligosaccharides.
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Duan, K.J., Sheu, D.C., Lin, M.T. et al. Reaction mechanism of isomaltooligosaccharides synthesis by α-glucosidase fromAspergillus carbonarious . Biotechnol Lett 16, 1151–1156 (1994). https://doi.org/10.1007/BF01020842
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DOI: https://doi.org/10.1007/BF01020842